Activated Phenoloxidase Interacts with a Novel Glycine-rich Protein on the Yeast Two-hybrid System

Journal of Biochemistry and Molecular Biology

Volumn 34, Issue 1, 2001, Pages 15-20

  Lee, Sun Woo ^a   Lee, Hyun Seong ^a   Kim, Eun Jun ^b   Yoo, Mi Ae ^a   Lee, Bok Luel ^a  

^a Pusan National University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Defense reaction; Melanin; Phenoloxidase; Prophenoloxidase; Yeast two hybrid

Indexed keywords

EID: 0035581451     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Ashida, M. and Brey, P (1998) Recent advances in research on the insect pro-phenol oxidases: in Molecular Mechanisms of Immune Responses in Insects (Brey, P E. and Hultmark, D., eds.), pp. 135-172, Chapman & Hall, London.
2. Aspan, A., Huang, T. S., Cerénius, L. and Söderhäll, K. (1995) cDNA cloning of prophenoloxidase from the freshwater crayfish Pacifastacus leniusculus and its activation. Proc. Natl. Acad. Sci. USA 92, 939-943.
3. Boman, H. G. (1991) Antibacterial peptides: key components needed in immunity. Cell 65, 205-207.
4. Cho, M. Y., Lee, H. S., Lee, K. M., Homma, K. I., Natori, S. and Lee, B. L. (1999a) Molecular cloning and functional properties of two early-stage encapsulation-relating proteins from the coleopteran insect, Tenebrio molitor larvae. Eur. J. Biochem. 262, 737-744.
5. Cho, M. Y., Choi, H., Moon, G. Y., Kim, M. H., Kwon, T. H., Homma, K., Natori, S. and Lee, B. L. (1999b) An 86 kDa diapause protein 1-like protein is a component of early-staged encapsulation-relating proteins in coleopteran insect, Tenebrio molitor larvae. FEBS Lett. 451, 303-307.
6. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
7. Dimopoulos, G., Richman, A., Muller, H. M. and Kafatos, F. C. (1997) Molecular immune responses of the mosquito Anopheles gambiae to bacteria and malaria parasites. Proc. Natl. Acad. Sci. USA 94, 11508-11513.
8. Hall, M., Scott, T., Sugumaran, M., Söderhäll, K. and Law, J. H. (1995) Proenzyme of Manduca sexta phenol oxidase: Purification, activation, substrate specificity of the active enzyme, and molecular cloning. Proc. Natl. Acad. Sci. USA 92, 7764-7768.
9. Hall, M., Wang, R., van Antwerpen, R., Sottrup-Jensen, L. and Söderhäll, K. (1999) The crayfish plasma clotting protein: a vitellogenin-related protein responsible for clot formation in crustacean blood. Proc. Natl. Acad. Sci. USA 96, 1965-1970.
10. Hoffmann, J. A., Reichhart, J. M. and Hetru, C. (1996) Innate immunity in higher insects. Curr. Opin. lmmunol. 8, 8-13.
11. Iwanaga, S., Kawabata, S. and Muta, T. (1998) New types of clotting factors and defense molecules found in horseshoe crab hemolymph: their structures and functions. J. Biochem. (Tokyo) 123, 1-15.
12. Janeway, C. A. (1989) Approaching the asymptote? Evolution and revolution in immunology. Cold Spring Harb. Symp. Quant. Biol. 54, 1-13.
13. Jung, Y. H., Park, B. Y., Lee, D. K., Hahn, Y. S., Chung, J. H., Han, D. M., Moon, H. J., Lee, B. L. and Lee, Y. H. (1995) Biochemical and molecular characterization of an antifungal protein from Tenebrio molitor larvae. Mol Cells 5, 287-292.
14. Kawabata, T., Yasuhara, Y., Ochiai, M., Matsuura, S. and Ashida, M. (1995) Molecular cloning of insect prophenoloxidase: A copper containing protein homologous to arthropod hemocyanin, Proc. Natl. Acad. Sci. USA 92, 7774-7778.
15. + channels by interaction with a family of membrane-associated guanylate kinases. Nature 378, 85-88.
16. Kwon, T. H., Kim, M. S., Choi, H. W., Joo, C. H., Cho, M. Y. and Lee, B. L. (2000) A masquerade-like serine proteinase homologue is necessary for phenoloxidase activity in the coleopteran insect, Holotrichia diomphalia larvae. Eur. J. Biochem. 267, 6188-6196.
17. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227, 680-685.
18. Lai-Fook, J. (1966) The repair of wounds in the integument of insects. J. Insect. Physiol. 12, 195-226.
19. Lee, H. S., Cho, M. Y., Lee, K. M., Kwon, T. H., Homma, K., Natori, S. and Lee, B. L. (1999) The pro-phenoloxidase of coleopteran insect, Tenebrio molitor, larvae was activated during cell clump/cell adhesion of insect cellular defense reactions. FEBS Lett. 444, 255-259.
20. Lee, H. Y., Hong, S. U., Oh, J. E., Kwon, M. Y., Yoon, J. H., Lee, J. H., Lee, B. L. and Moon, H. M. (1998a) Identification and characterization of the antimicrobial peptide corresponding to C-terminal b-sheet domain of tenecin 1, an antibacterial protein of larvae of Tenebrio molitor. Biochem. J. 334, 99-105.
21. Lee, K. M, Lee, K. Y, Choi, H. W., Cho, M. Y., Kwon, T. H., Kawabata, S. and Lee, B. L. (2000) Activated phenoloxidase from Tenebrio molitor larvae enhances the synthesis of melanin by using a vitellogenin-like protein in the presence of dopamine. Eur. J. Biochem. 267, 3695-3703.
22. Lee, S. Y., Moon, H. J., Kurata, S., Kurama, T., Natori, S. and Lee, B. L. (1994) Purification and molecular cloning of cDNA for an inducible antibacterial protein of larvae of a coleopteran insect, Holotrichia diomphalia. J. Biochem. (Tokyo), 115, 82-86.
23. Lee, S. Y., Moon, H. J., Kurata, S., Natori, S. and Lee, B. L. (1995) Purification and cDNA cloning of an antifungal protein from the hemolymph of Holotrichia diomphalia larvae. Biol. Pharm. Bull. 18, 1049-1052.
24. Lee, S. Y., Kwon, T. H., Hyun, J. H., Choi, J. S., Kawabata, S. L, Iwanaga, S. and Lee, B. L. (1998b) In vitro activation of pro-phenol-oxidase by two kinds of pro-phenoloxidase-activating factors isolated from hemolymph of coleopteran, Holotrichia diomphalia larvae. Eur. J. Biochem. 254, 50-57.
25. Lee, S. Y., Cho, M. Y., Hyun, J. H., Lee, K. M., Homma, K., Natori, S., Kawabata, S., Iwanaga, S. and Lee, B. L. (1998c) Molecular cloning of cDNA for pro-phenol-oxidase-activating factor I, a serine protease is induced by lipopolysaccharide or 1,3-beta-glucan in coleopteran insect, Holotrichia diomphalia larvae. Eur. J. Biochem. 257, 615-621.
26. Lee, S. Y., Wang, R. and Söderhäll, K. (2000) A lipopolysaccharide and β-1,3-glucan-binding protein from hemocytes of the freshwater crayfish Pacifastacus leniusculus. J. Biol. Chem. 275, 1337-1343.
27. Medzhitov, R. and Janeway, C, A. (1997a) Innate immunity: the virtue of a non-clonal system of recognition. Cell 91, 295-298.
28. Medzhitov, R. and Janeway, C, A. (1997b) Innate immunity: impact on the adaptive immune response. Curr. Opin. Immunol. 9, 4-9.
29. Moon, H. J., Lee, S. Y., Kurata, S., Natori, S. and Lee, B. L. (1994) Purification and molecular cloning of cDNA for an inducible antibacterial protein from larvae of the coleopteran, Tenebrio molitor. J. Biochem. (Tokyo) 116, 53-58.
30. Natori, S. (1994) Function of antimicrobial proteins in insects. Ciba Found. Symp. 186, 123-132.
31. Ochiai, M. and Ashida, M. (1998) Purification of a β-1,3-glucan recognition protein in the prophenoloxidase activating system from hemolymph of the silkworm, Bombyx mori. J. Biol. Chem. 263, 12056-12062.
32. Richman, A. and Kafatos, F. C. (1996) Immunity to eukaryotic parasites in vector insects. Curr. Opin. Immunol. 8, 14-19.
33. Rowley, A. F. and Ratcliffe, N. A. (1981) Insects: Invertebrate Blood Cells (Rowley, A. F. and Ratcliffe, N. A., eds.), Academic Press, London.
34. Söderhäll, K. and Cerenius, L. (1998) Role of the prophenoloxidase-activating system in invertebrate immunity. Curr. Opin. Immunol. 10, 23-28.
35. Sugumaran, M (1991) Molecular mechanisms for mammalian melanogenesis: Comparison with insect cuticular sclerotization. FEBS Lett. 295, 233-239.
36. Thomas, P. S. (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77, 5201-5205.