Functional expression and cellular localization of a green fluorescent protein-tagged proline transporter in Aspergillus nidulans

Fungal Genetics and Biology

Volumn 33, Issue 2, 2001, Pages 115-125

  Tavoularis, Stefanos ^a   Scazzocchio, Claudio ^b   Sophianopoulou, Vicky ^a  

^a INSTITUTE OF NANOSCIENCE AND NANOTECHNOLOGY   (Greece)

^b INSTITUTE FOR INTEGRATIVE BIOLOGY OF THE CELL I2BC   (France)

Author keywords

Aspergillus; Green fluorescent protein; Permease; Proline; Proline transport

Indexed keywords

AMINO ACID; CARRIER PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROLINE; PROTEIN PRNB; UNCLASSIFIED DRUG;

AMINO ACID TRANSPORT; ARTICLE; ASPERGILLUS NIDULANS; CARBOXY TERMINAL SEQUENCE; CHIMERA; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CORRELATION ANALYSIS; GENE FUSION; GENE LOCUS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; TEMPERATURE;

ASPERGILLUS; ASPERGILLUS NIDULANS; EMERICELLA NIDULANS; EUKARYOTA; PROKARYOTA;

EID: 0035572666     PISSN: 10871845     EISSN: None     Source Type: Journal    
DOI: 10.1006/fgbi.2001.1280     Document Type: Article

References (37)

1. Functional characterization of a maize purine transporter by expression in Aspergillus nidulans
2. A rapid and sensitive method for quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding
3. Engineered GFP as a vital reporter in plants
4. The induction and repression of nitrate reductase in the fungus Aspergillus nidulans
5. Metabolite repression and inducer exclusion in the proline utilization gene cluster of Aspergillus nidulans
6. Understanding, improving and using green fluorescent proteins
7. Plant adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus Aspergillus nidulans
8. Ubiquitin Lys63 is involved in ubiquitination of a yeast plasma membrane protein
9. 'ER degradation' of a mutant yeast plasma membrane protein by the ubiquitin-proteasome pathway
10. The proline utilization gene cluster of Aspergillus nidulans
11. Identification of an ER retrieval signal in a retroviral glycoprotein
12. The integration of nitrogen and carbon catabolite repression in Aspergillus nidulans requires the GATA factor Area and an additional positive-acting element
13. NPI1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes an Rsp5 ubiquitin-protein ligase
14. Regulation of inositol transport in Saccharomyces cerevisiae involves inositol induced changes in permease stability and endocytic degradation in the vacuole
15. Cloning and characterization of the ethanol utilisation regulon in Aspergillus nidulans
16. A PEST-like sequence mediates phosphorylation and efficient ubiquitination of yeast uracil permease
17. Characterization of the glucose-induced inactivation of maltose permease in Saccharomyces cerevisiae
18. Post-translational fate of CAN1 permease of Saccharomyces cerevisiae
19. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy
20. Intracellular localization of an active green fluorescent protein-tagged Pho94 phosphate permease in Saccharomyces cerevisiae
21. The analysis of the transcriptional activator PrnA reveals a tripartite nuclear localization sequence
22. Transformation of Aspergillus nidulans on the hygromycin B resistance marker from Escherchia coli
23. Physiological regulation of membrane protein sorting late in the secretory pathway of Saccharomyces cerevisiae
24. The inmunodetected yeast purine-cytosine permease is not glycosylated nor are glycosylation signals required to have a functional permease
25. Uracil-induced down-regulation of the yeast uracil permease
26. Amino acid transporters of lower eukaryotes: Regulation, structure and topogenesis
27. The proline permease of Aspergillus nidulans is very similar to amino-acid permeases of Saccharomyces cerevisiae
28. Operator derepressed mutations in the proline utilization gene cluster of Aspergillus nidulans
29. Nuclear traffic in fungal hyphae: In vivo study of nuclear migration and positioning in Aspergillus nidulans
30. Post-transcriptional regulation and kinetic characterization of proline transport in germinating conidiospores of Aspergillus nidulans
31. The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation
32. Transformation by integration in Aspergillus nidulans
33. Immunological detection and subcellular localization of the general purine transporter UapC from Aspergillus nidulans
34. Endocytosis and degradation of the yeast uracil permease under adverse conditions