A spectrophotometric analysis of pigments in apples

Russian Journal of Plant Physiology

Volumn 48, Issue 5, 2001, Pages 693-700

  Solovchenko, A E ^a   Chivkunova, O B ^a   Merzlyak, M N ^a   Reshetnikova, I V ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

Anthocyanins; Carotenoids; Chlorophylls; Malus domestica; Spectrophotometry

Indexed keywords

MALUS X DOMESTICA;

EID: 0035538045     PISSN: 10214437     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016780624280     Document Type: Article

References (25)

1. Knee, M., Anthocyanin, Carotenoid, and Chlorophyll Changes in Peel of Cox's Orange Pippin Apples during Ripening on and off the Tree, J. Exp. Bot., 1972, vol. 23, pp. 184-196.
2. Knee, M., Effect of Storage Treatments upon the Ripening of Conference Pears, J. Sci. Food Agric., 1973, vol. 24, pp. 1137-1145.
3. Hendry, G.A.F., Houghton, J.D., and Brown, S.B., The Degradation of Chlorophyll - a Biological Enigma, New Phytol., 1987, vol. 107, pp. 255-302.
4. Brown, S.B., Horghton, J.D., and Hendry, C.A.F., Chlorophyll Breakdown, Chlorophylls, Sheer, H., Ed., Boca Raton: CRC, 1991, pp. 465-489.
5. Knee, M., Carotenol Esters in Developing Apple Fruits, Photochemistry, 1988, vol. 27, pp. 1005-1009.
6. Merzlyak, M.N., Gitelson, A.A., Chivkunova, O.B., and Rakitin, V.Yu., Nondestructive Optical Detection of Pigment Changes during Leaf Senescence and Fruit Ripening, Physiol. Plant., 1999, vol. 106, pp. 135-141.
7. Merzlyak, M.N. and Chivkunova, O.B., Light-Stress-Induced Pigment Change and Evidence for Anthocyanin Photoprotection in Apples, J. Photochem. Photobiol., vol. 55, pp. 154-162.
8. Lancaster, J.E., Grant, J.E., Lister, C.E., and Taylor, M.C., Skin Color in Apples - Influence of Copigrnentation and Plastid Pigments in Shade and Darkness of Red Color in Five Genotypes, J. Am. Soc. Hort. Sci., 1994, vol. 119, pp. 63-69.
9. Markham, K.R., Flavones, Flavonols and Their Glycosides, Methods in Plant Biochemistry, Harborne, J.B. and Dey, P.M., Eds., London: Academic, 1989, vol. 1, pp. 197-232.
10. Strack, D. and Wray, V., Anthocyanins, Methods in Plant Biochemistry, Harborne, J.B. and Dey, P.M., Eds., London: Academic, 1989, vol. 1, pp. 326-352.
11. Escarpa, A. and Gonzales, M.C., High-Performance Liquid Chromatography with Diode-Array Detection for the Determination of Phenolic Compounds in Peel and Pulp from Different Apple Varieties, J. Chromatogr., 1998, vol. 823, pp. 331-337.
12. Chalker-Scott, L., Environmental Significance of Anthocyanins in Plant Stress Responses, Photochem. Photobiol., 1999, vol. 70, pp. 1-9.
13. Lichtenthaler, H.K., Chlorophyll and Carotenoids: Pigments of Photosynthetic Biomembranes, Methods Enzymol., 1987, vol. 148, pp. 331-382.
14. Porra, R.A., Recent Advances and Re-Assessment in Chlorophyll Extraction and Assay Procedures for Terrestrial, Aquatic and Marine Organisms, Including Recalcitrant Algae, Chlorophylls, Sheer, H., Ed., Boca Raton: CRC, 1991, pp. 31-57.
15. Merzlyak, M.N., Chivkunova, O.B., Lekhirnena, L., and Belevich, N.P., Some Limitation and Potentialities of the Spectrophotometric Assay of Pigment Extracted from Leaves of Higher Plants, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 926-936 (Russ. J. Plant Physiol., Engl. Transl.).
16. Bernstein, I.Ya. and Kaminskii, Yu.L., Spektrofotometricheskii analiz v organicheskoi khimii (Spectrophotometric Analysis in Organic Chemistry), Leningrad: Khimiya, 1986.
17. Chivkunova, O.B., Merzlyak, M.N., Ponotnareva, N.P., and Gudkovskii, V.A., Cuticular Lipids, α-Farnezene, Products of Its Oxidation, and the Development of Red Blush on Apple Fruits, Prikl. Biokhim. Mikrobiol., 1997, vol. 33, pp. 439-444.
18. Metlitskii, L.V., Osnovy hiokhimii plodov i ovoshchei (The Basics of Biochemistry of Fruits and Vegetables), Moscow: Ekonomika, 1976.
19. Wellburn, A.R., The Spectral Determination of Chlorophylls a and b, as Well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution, J. Plant Physiol., 1994, vol. 144, pp. 307-313.
20. Folch, J., Lees, M., and Sloane Stanley, G.H., A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem., 1957, vol. 226, pp. 497-509.
21. Merzlyak, M.N., Kovrizhnikh, V.A., and Kaurov, Yu.N., Improved Solvent System for Plant Pigment Separation on Silica Gel Thin Layers, J. Chromatogr., 1983, vol. 262, pp. 331-333.
22. Merzlyak, M.N., Khozin, I., and Cohen, Z., Spectrophotometric Analysis of Carotenoids in Plant Extracts Based on Elimination of Chlorophyll Absorption, Phytochem. Anal., 1996, vol. 7, pp. 294-299.
23. Naqvi, K.B., Meló, T.B., and Raji, B.B., Assaying of Chromophore Composition of Photosynthetic Systems by Spectral Resolution: Application to the Light-Harvesting Complex (LHC II) and Total Pigment Content of Higher Plants, Spectrochim. Acta, 1997, vol. 53, pp. 2229-2234.
24. Britton, G., UV/Visible Spectroscopy, Carotenoids, Britton, G., Liaaen-Jensen, S., and Pfander, H., Eds., Basel: Birkhaüser, 1995.
25. Reay, P.P., The Role of Low Temperatures in the Development of the Red Blush on Apple Fruit ("Granny Smith"), Sci. Hortic., 1999, vol. 79, pp. 113-119.