Criteria for evaluation of the immunoblot in the diagnosis of Lyme Borreliosis using Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu stricto

Epidemiologie, Mikrobiologie, Imunologie

Volumn 50, Issue 4, 2001, Pages 147-156

  Honegr, K ^a   Havlasova J ^a   Gebousky P ^a   Dostal P ^a   Pellantova V ^a   Skrabkova Z ^a   Hulinska D ^a  

^a UNIVERSITY HOSPITAL HRADEC KRALOVE   (Czech Republic)

Author keywords

Borrelia burgdorferi sensu lato; Immunoblot; Lyme borreliosis

Indexed keywords

BACTERIAL ANTIGEN; BACTERIUM ANTIBODY; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; DIAGNOSTIC AGENT;

ADOLESCENT; ADULT; AGED; ARTICLE; BLOOD ANALYSIS; BLOOD SAMPLING; BORRELIA AFZELII; BORRELIA BURGDORFERI; BORRELIA GARINII; CHILD; CONTROLLED STUDY; ENDEMIC DISEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENOTYPE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOBLOTTING; LYME DISEASE; MAJOR CLINICAL STUDY; MALE; MEDICAL ASSESSMENT; MICROBIOLOGICAL EXAMINATION; REGRESSION ANALYSIS; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING; IMMUNOLOGY; PRESCHOOL CHILD; SEROLOGY; STATISTICAL MODEL;

ADOLESCENT; ADULT; AGED; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BLOTTING, WESTERN; BORRELIA BURGDORFERI GROUP; CHILD; CHILD, PRESCHOOL; ENGLISH ABSTRACT; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; LOGISTIC MODELS; LYME DISEASE; MALE; MIDDLE AGE; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; SUPPORT, NON-U.S. GOV'T;

EID: 0035512513     PISSN: 12107913     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Evolution of the serologic response to Borrelia burgdorferi in treated patients with culture-confirmed erythema migrans
2. Laboratory aspects of Lyme borreliosis
3. Serodiagnosis of early Lyme disease: Evaluation of IgM and IgG antibody responses by antibody capture enzyme immunoassay
4. Evaluation of a Lyme disease enzyme immunoassay using the 41-G fragment of flagellin
5. Antibody responses to the three genomic groups of Borrelia burgdorferi in European Lyme borreliosis
6. Western blotting in the serodiagnosis of Lyme disease
7. European interlaboratory comparison of Lyme borreliosis serology
8. Interpretation criteria for standardized western blots for three European species of Borrelia burgdorferi sensu lato
9. Validity of interpretation criteria for standardized Western blots (immunoblots) for serodiagnosis of Lyme borreliosis based on sera collected throughout Europe
10. Recommendation to include OspA and OspB in the new immunoblotting criteria for serodiagnosis of Lyme disease
11. Užití a standardizace Western blotu při lymeské borelióze
12. Electron microscopy of Langerhans cells and Borrelia burgdorferi in Lyme disease patients
13. Surveillance of Lyme borreliosis in Czech Republic
14. Enzyme-linked immunosorbent assays for Lyme disease: Reactivity of subunits of Borrelia burgdorferi
15. Species-specific plasmid sequences for PCR identification of the three species of Borrelia burgdorferi sensu lato involved in Lyme disease
16. Utilization of tests for lyme disease antibody at a university hospital
17. Serodiagnosis of Lyme borreliosis by Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii Western Blots (immunoblots)
18. Antibodies against whole sonicated Borrelia burgdorferi spirochetes, 41-kilodalton flagellin, and P39 protein in patients with PCR- or culture-proven late Lyme borreliosis
19. The polymerace chain reaction in the diagnosis of Lyme neuroborreliosis
20. A European multicenter study of immunoblotting in serodiagnosis of Lyme borreliosis
21. Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi
22. Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease
23. Scored antibody reactivity determined by immunoblotting shows an association between clinical manifestations and presence of Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, and B. valaisiana in humans
24. Comparison of seven commercial kits for detection of antibodies to Borrelia burgdorferi
25. Erythema migrans and serodiagnosis by enzyme immunoassay and immunoblot with three borrelia species
26. Polymorphism in OspC gene of Borrelia burgdorferi and immunoreactivity of OspC protein: Implications for taxonomy and for use of OspC protein as a diagnostic antigen
27. The western immunoblot for Lyme disease: Determination of sensitivity, specificity, and interpretive criteria with use of commercially available performance panels
28. Molecular subtyping of Borrelia burgdorferi in erythema migrans and acrodermatitis chronica atrophicans
29. Genospecies and their influence on immunoblot results
30. Detection of IgM and IgG antibodies to Borrelia burgdorferi using different strains
31. Immunochemical and immunological analysis of European Borrelia burgdorferi strains
32. Recombinant immunoblot in the serodiagnosis of Lyme borreliosis. Comparison with indirect immunofluorescence and enzyme-linked immunosorbent assay
33. An improved recombinant IgG immunoblot for serodiagnosis of Lyme borreliosis
34. A limitation of 2-stage serological testing for Lyme disease: Enzyme immunoassay and immunoblot assay are not independent tests
35. Validity of western immunoblot band patterns in the serodiagnosis of Lyme borreliosis