Quantitative analysis by flow cytometry of abscisic acid-inducible gene expression in transiently transformed rice protoplasts

Cytometry

Volumn 45, Issue 3, 2001, Pages 170-179

  Hagenbeek, Dik ^a   Rock, Christopher D ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

glucuronidase; ABA insensitive; Abi1; Abscisic acid; Arabidopsis thaliana; Arabinogalactan protein; Flow cytometry; Gene expression; GFP; Green fluorescent protein; GUS; Lanthanum; Luciferase; Maize; Oryza sativa; Protoplasts; Reporter gene; Rice; Transient transformation; Triticum aestivum; Viviparous; Vp1; Wheat; Zea mays

Indexed keywords

ABSCISIC ACID; ARABINOGALACTAN; BETA GLUCURONIDASE; COMPLEMENTARY DNA; EPITOPE; GLYCOPROTEIN; GREEN FLUORESCENT PROTEIN; LANTHANUM CHLORIDE; LUCIFERASE; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; FLOW CYTOMETRY; GENE CONSTRUCT; GENE EXPRESSION REGULATION; INTERMETHOD COMPARISON; MARKER GENE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTOPLAST; REPORTER GENE; REPRODUCIBILITY; RICE;

ABSCISIC ACID; FLOW CYTOMETRY; GENE EXPRESSION; GENES, REPORTER; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; LUCIFERASES; LUMINESCENT PROTEINS; MEMBRANE GLYCOPROTEINS; ORYZA SATIVA; PROTOPLASTS; TRANSFORMATION, GENETIC;

ARABIDOPSIS THALIANA; ORYZA SATIVA; TRITICUM AESTIVUM; ZEA MAYS;

EID: 0035504129     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20011101)45:3<170::AID-CYTO1160>3.0.CO;2-Z     Document Type: Article

References (49)

1. Preparation and transformation of monocot protoplasts
2. Assaying chimeric genes in plants: The GUS gene fusion system
3. Fusions to βlactamase as a reporter for gene expression in live mammalian cells
4. Green-fluorescent protein as a new vital marker in plant cells
5. Engineered GFP as a vital reporter in plants
6. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants
7. Reporter gene expression for monitoring gene transfer
8. The green fluorescent protein
9. Measurement of green fluorescence protein in plants: Quantification, correlation to expression, rapid screening and differential gene expression
10. Aequorea green fluorescent protein: Simultaneous analysis of wild-type and blue-fluorescing mutant by flow cytometry
11. Simultaneous analysis of the cyan, yellow and green fluorescent proteins by flow cytometry using single-laser excitation at 458 nm
12. Fluorescent proteins in single- and multicolor flow cytometry
13. Simultaneous flow cytometric analyses of enhanced green and yellow fluorescent proteins and cell surface antigens in doubly transduced immature hematopoietic cell populations
14. Flow cytometric analysis and FACS sorting of cells based on GFP accumulation
15. Flow cytometric analysis of transgene expression in higher plants: Green fluorescent protein
16. Abscisic acid signal transduction
17. Genetic control and integration of maturation and germination pathways in seed development
18. Gene expression regulated by abscisic acid and its relation to stress tolerance
19. The molecular basis of dehydration tolerance in plants
20. Pathways to abscisic acid-regulated gene expression
21. Regulation of a wheat promoter by abscisic acid in rice protoplasts
22. Promoter switches specific for abscisic acid (ABA)-induced gene expression in cereals
23. Regulation of abscisic acid-induced transcription
24. Arabidopsis ABA response gene ABI1: Features of a calcium-modulated protein phosphatase
25. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana
26. The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator
27. The ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling
28. Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants
29. Integrated control of seed maturation and germination programs by activator and repressor functions of Viviparous-1 of maize
30. 14-3-3 Proteins are part of an abscisic acid-VIVIPAROUS1 (Vp1) response complex in the Em promoter and interact with Vp1 and EMBP1
31. A bZIP factor, TRAB1, interacts with Vp1 and mediates abscisic acid-induced transcription
32. Identification and analysis of proteins that interact with the Avena fatua homologue of the maize transcription factor VIVIPAROUS1
33. Trivalent ions activate abscisic acid-inducible promoters through an ABI1-dependent pathway in rice protoplasts
34. Functional interactions of lanthanum and phospholipase D with the abscisic acid signaling effectors VP1 and ABI1-1 in rice protoplasts
35. Flow cytometry and surface plasmon resonance analyses demonstrate that the monoclonal antibody JIM19 interacts with a rice cell surface component involved in abscisic acid signaling in protoplasts
36. A conserved domain of the viviparous-1 gene product enhances the DNA binding activity of the BZIP protein EmBP-1 and other transcription factors
37. Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation
38. Expression in plants of 2 bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector
39. The sensitivity of barley aleurone tissue to gibberellin is heterogeneous and may be spatially determined
40. Comparative study of five commercial reagents for preparing normal and leukaemic lymphocytes for immunophenotypic analysis by flow cytometry
41. Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols
42. Co-transformation of unlinked foreign genes into plants by direct gene transfer
43. Competence for gene transfer by electroporation in a sub-population of protoplasts from uniform carrot cell suspension cultures
44. Factors affecting transient gene expression in electroporated Glycine max protoplasts
45. Non-radioactive detection of β-glucuronidase and chloramphenicol acetyltransferase activities in co-transformed protoplasts by HPLC
46. Spectral variants of green fluorescent protein
47. A chimeric reporter gene allowing for clone selection and high-throughput screening of reporter cell lines expressing G-protein-coupled receptors
48. Green fluorescence protein as a transcriptional reporter for the long terminal repeats of the human immunodeficiency virus type 1
49. The monoclonal antibody JIM19 modulates abscisic acid action in barley aleurone protoplasts