SDS-subtilisin catalyzed synthesis of tetra-peptides containing multifunctional amino acid residues in ethanol

Journal of Molecular Catalysis - B Enzymatic

Volumn 15, Issue 4-6, 2001, Pages 105-110

  Getun, Irina V ^a   Filippova, Irina Yu ^a   Lysogorskaya, Elena N ^a   Oksenoit, Elena S ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

Enzymatic peptide synthesis; Multifunctional amino acids; Organic solvent; SDS subtilisin; Subtilisin

Indexed keywords

AMINO ACIDS; CATALYSIS; ETHANOL; SYNTHESIS (CHEMICAL);

ACYL DONOR STRUCTURES;

ENZYMES;

ALCOHOL; AMINO ACID; BENZYLOXYCARBONYLALANYLALANYLARGINYLARGINYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLARGINYLGLUTAMYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLARGINYLPHENYLALANYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLASPARTYLARGINYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLASPARTYLGLUTAMYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLASPARTYLPHENYLALANYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLGLUTAMYLARGINYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLGLUTAMYLGLUTAMYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLGLUTAMYLPHENYLALANYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLHISTIDYLARGINYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLHISTIDYLGLUTAMYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLHISTIDYLPHENYLALANYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLLEUCYLPHENYLALANYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLLYSYLARGINYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLLYSYLGLUTAMYL 4 ASPARAGINYLALANINE; BENZYLOXYCARBONYLALANYLALANYLLYSYLPHENYLALANYL 4 ASPARAGINYLALANINE; SUBTILISIN; TETRAPEPTIDE; UNCLASSIFIED DRUG;

ACYLATION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CATALYST; PEPTIDE SYNTHESIS;

EID: 0035501619     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1381-1177(01)00013-3     Document Type: Article

References (14)

1. Proteinases as catalysts in peptide synthesis
2. The synthesis of tri- and tetra-peptides catalyzed by subtilisin suspensions in organic solvents
3. Peptide synthesis catalyzed by subtilisin 72 in organic solvents
4. Structure and stability of insulin dissolved in 1-octanol
5. Generation of soluble and active subtilisin and α-chymotrypsin in organic solvents via hydrophobic ion pairing
6. SDS-subtilisin complex efficiently catalyzes synthesis of peptides in ethanol and 2-propanol
7. Subtilisin 72: A serine protease from Bacillus subtilis strain 72 - An enzyme similar to subtilisin Carlsberg
8. Effects of temperature and pH on the stability and catalytic activity of serine proteinase from Bacillus subtilis strain
9. Remarkable effects of donor esters on the α-chymotrypsin-catalyzed couplings of inherently poor amino acid substrates
10. Superiority of the carbamoylmethyl ester as donor ester in the protease catalyzed kinetically controlled peptide synthesis in organic media
11. Exploitation of subtilisin BPN as catalyst for the synthesis of peptides containing non-coded amino acids, peptide mimetics and peptide conjugates
12. The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 Å resolution
13. Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching
14. Subtilisin from Bacillus subtilis strain 72: The influence of substrate structure, temperature and pH on catalytic properties