A fluorometric assay for the determination of 1-deoxy-D-xylulose 5-phosphate synthase activity

Analytical Biochemistry

Volumn 296, Issue 1, 2001, Pages 101-105

  Querol, Jordi ^a   Besumbes, Oscar ^a   Maria Lois, Luisa ^a   Boronat, Albert ^a   Imperial, Santiago ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

1 deoxy D xylulose 5 phosphate synthase; 3,5 diaminobenzoic acid; Fluorometric assay; Isoprenoid biosynthesis

Indexed keywords

BIOCHEMISTRY; MIXTURES;

ACIDIC MEDIUMS; ACTIVITY-BASED; FLUORESCENCE INTENSITIES; FLUOROMETRIC ASSAY; FUNCTIONAL CHARACTERIZATION; ISOPRENOID BIOSYNTHESIS; REACTION MIXTURE; SYNTHASES;

FLUORESCENCE;

1 DEOXY DEXTRO XYLULOSE 5 PHOSPHATE; 1 DEOXY DEXTRO XYLULOSE 5 PHOSPHATE SYNTHASE; 3,5 DIAMINOBENZOIC ACID; ANTIBIOTIC AGENT; BENZOIC ACID DERIVATIVE; ENZYME INHIBITOR; HERBICIDE; ISOPRENOID; SYNTHETASE; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; ASSAY; DRUG ACTIVITY; DRUG DETERMINATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME MECHANISM; FLUOROMETRY; PRIORITY JOURNAL; RADIOMETRY; REACTION TIME; SCREENING; VALIDATION PROCESS;

EID: 0035450949     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5234     Document Type: Article

References (24)

1. Isoprenoid biosynthesis in bacteria: A novel pathway for the early steps leading to isopentenyl diphosphate
2. Glyceraldehyde 3-phosphate and pyruvate as precursors of isoprenic units in an alternative non-mevalonate pathway for terpenoid biosynthesis
3. Terpenoid biosynthesis from 1-deoxy-D-xylulose in higher plants by intra molecular skeletal rearrangement
4. A family of transketolases that directs isoprenoid biosynthesis via a non-mevalonate-independent pathway
5. A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative non mevalonate pathway for terpenoid biosynthesis
6. Isoprenoid biosynthesis via the mevalonate-independent route, a novel target for antibacterial drugs?
7. The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants
8. The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants
9. Identification of a novel thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
10. Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis
11. 1) in higher plant chloroplasts
12. 1)
13. Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs
14. 1-Deoxy-D-xylulose 5-phosphate synthase, the gene product of open reading frame (ORF) 2816 and ORF 2895 in Rhodobacter capsulatus
15. Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis
16. Tools for discovery of inhibitors of the 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: An approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosa
17. Fluorimetric assay for 1-deoxyfructose
18. Rapid and efficient site-specific mutagenesis without phenotypic selection
19. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
20. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
21. Identification and rejection of outliners in enzyme kinetics