Use of the monoclonal antibody DAKO-ERβ (8D5-1) to measure oestrogen receptor beta in breast cancer cells

Cytometry

Volumn 45, Issue 1, 2001, Pages 65-72

  Girdler, F ^a   Browell, D A ^b   Cunliffe, W J ^b   Shenton, B K ^a   Hemming, J D ^b   Scorer, P ^b   Young, J R ^b   Brotherick, I ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b QUEEN ELIZABETH HOSPITAL   (United Kingdom)

Author keywords

Breast cancer cells; ER ; Monoclonal antibody; Paraformaldehyde fixed cells

Indexed keywords

ESTROGEN RECEPTOR BETA; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 8D5 1; UNCLASSIFIED DRUG;

ACCURACY; ANTIBODY DETECTION; ANTIBODY SPECIFICITY; ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; CROSS REACTION; FLOW CYTOMETRY; HORMONAL THERAPY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR BINDING; WESTERN BLOTTING;

ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; BREAST NEOPLASMS; ESTROGEN RECEPTOR BETA; FLOW CYTOMETRY; HUMANS; IMMUNOHISTOCHEMISTRY; RECEPTORS, ESTROGEN; TUMOR CELLS, CULTURED;

EID: 0035450486     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20010901)45:1<65::AID-CYTO1145>3.0.CO;2-8     Document Type: Article

References (36)

1. The role of steroid hormones and growth factors in the control of normal and malignant breast
2. The oestrogen receptor: From perception to mechanism
3. Changes in estrogen-receptor, progesterone-receptor, and pS2 expression in Tamoxifen-resistant human breast-cancer
4. Exon-5 deletion variant estrogen-receptor messenger-RNA expression in relation to Tamoxifen resistance and progesterone-receptor-pS2 status in human breast-cancer
5. Antagonistic and agonistic effects of tamoxifen: Significance in human cancer
6. Drug-resistance to Tamoxifen during breast-cancer therapy
7. Cloning of a novel estrogen receptor expressed in rat prostate and ovary
8. ER beta: Identification and characterization of a novel human estrogen receptor
9. Cloning, chromosomal localization, and functional analysis of the murine estrogen receptor beta
10. Localization of the human oestrogen receptor gene to chromosome 6q24 · q27 by in situ hybridization
11. A novel human estrogen receptor beta: Identification and functional analysis of additional N-terminal amino acids
12. Human estrogen receptor beta-gene structure, chromosomal localization, and expression pattern
13. The novel estrogen receptor-beta subtype: Potential role in the cell- and promoter-specific actions of estrogens and anti-estrogens
14. Differential ligand activation of estrogen receptors ER alpha and ER beta at AP1 sites
15. Human estrogen receptor beta binds DNA in a manner similar to and dimerizes with estrogen receptor alpha
16. Mouse estrogen receptor beta forms estrogen response element-binding heterodimers with estrogen receptor alpha
17. The complete primary structure of human estrogen receptor beta (hER beta) and its heterodimerization with ER alpha in vivo and in vitro
18. Use of the biotinylated antibody DAKO-ER 1D5 to measure estrogen- receptor on cytokeratin positive cells obtained from primary breast-cancer cells
19. The effect of 3-week tamoxifen treatment on oestrogen receptor levels in primary breast tumours: A flow cytometric study
20. Are fine-needle breast aspirates representative of the underlying solid tumor - A comparison of receptor levels, ploidy and the influence of cytokeratin gates
21. Influence of estrogen-receptors on survival and recurrence in patients with breast-cancer without lymph-node metastases
22. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta
23. Expression of estrogen receptor-beta in human breast tumors
24. Expression of estrogen receptor beta 1, beta 2, and beta 5 messenger RNAs in human breast tissue
25. Altered estrogen receptor alpha and beta messenger RNA expression during human breast tumorigenesis
26. Coexpression of estrogen receptor alpha and beta: Poor prognostic factors in human breast cancer?
27. Increased expression of estrogen receptor beta mRNA in tamoxifen- resistant breast cancer patients
28. Expression levels of estrogen receptor-alpha, estrogen receptor-beta, coactivators, and corepressors in breast cancer
29. Estrogen inhibits the vascular injury response in estrogen receptor alpha-deficient mice
30. Expression of oestrogen receptor beta (ER beta) in multiple rat tissues visualised by immunohistochemistry
31. Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen- receptor gene
32. Generation and reporoductive phenotypes of mice lacking estrogen receptor beta
33. Functional estrogen receptor beta in colon cancer cells
34. Activation of transcription by estrogen receptor alpha and beta is cell type- and promoter-dependent
35. Expression of wild-type estrogen receptor beta and variant isoforms in human breast cancer
36. Estrogen receptors alpha and beta in the rodent mammary gland