Quantification of Escherichia coli genomic DNA contamination in recombinant protein preparations by polymerase chain reaction and affinity-based collection

Analytical Biochemistry

Volumn 296, Issue 1, 2001, Pages 114-121

  Gregory, Carl A ^a   Rigg, Gordon P ^a   Illidge, Christopher M ^a   Matthews, Ruth C ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

DNA contamination; E. coli; ELISA; PCR; Recombinant protein

Indexed keywords

DNA; ESCHERICHIA COLI; GENES; RECOMBINANT PROTEINS;

DNA QUANTIFICATION; E. COLI; ELISA; GENES ENCODING; LINEAR RESPONSE; PCR AMPLIFICATION; PROTEIN PREPARATIONS; TRADITIONAL FILTER;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; RECOMBINANT PROTEIN; RNA 5S;

ACCURACY; ARTICLE; BACTERIAL GENE; BINDING AFFINITY; CONTAMINATION; CONTROLLED STUDY; DILUTION; DNA BINDING; DNA DETERMINATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXTRACTION; GENE AMPLIFICATION; HYBRIDIZATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RADIOACTIVITY; TECHNIQUE; VALIDATION PROCESS;

ESCHERICHIA COLI;

EID: 0035450197     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5237     Document Type: Article

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