Improvement of freezing tolerance in transgenic tobacco leaves by expressing the hiC6 gene

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 8, 2001, Pages 1796-1804

  Honjoh, Ken Ichi ^a   Shimizu, Hideyuki ^a   Nagaishi, Noriko ^a   Matsumoto, Hiroko ^a   Suga, Koushirou ^a,b   Miyamoto, Takahisa ^a   Iio, Masayoshi ^a   Hatano, Shoji ^c  

^a KYUSHU UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c NISHIKYUSHU UNIVERSITY   (Japan)

Author keywords

Chlorella vulgaris C 27; Freezing tolerance; LEA protein; Nicotiana tabacum; Transgenic plants

Indexed keywords

CHLORELLA VULGARIS; MIMOSA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; TABACUM;

ALGAL PROTEIN; ELECTROLYTE;

ARTICLE; BIOSYNTHESIS; CELL FRACTIONATION; CHEMISTRY; CHLORELLA; ENZYMOLOGY; FREEZING; GENETICS; IN SITU HYBRIDIZATION; METABOLISM; PHYSIOLOGY; PLANT LEAF; PLASMID; SOUTHERN BLOTTING; TOBACCO; TRANSGENIC PLANT;

ALGAL PROTEINS; BLOTTING, SOUTHERN; CHLORELLA; ELECTROLYTES; FREEZING; IN SITU HYBRIDIZATION; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PLASMIDS; SUBCELLULAR FRACTIONS; TOBACCO;

EID: 0035432997     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1796     Document Type: Article

References (35)

1. Guy, C. L., Cold acclimation and freezing tolerance: role of protein metabolism. Annu. Rev. Plant Physiol. Plant MoIl Bio., 41, 187-223 (1990).
2. Thomashow, M. F., Molecular genetics of cold acclimation in higher plants. Adv. Genet., 28, 99-131 (1990).
3. Gilmour, S. J., Artus, N. N., and Thomashow, M. F., cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana. Plant Mol. Biol., 18, 13-21 (1992).
4. Houde, M., Danyluk, J., Laliberte, J.-F., Rassart, E., Dhindsa, R. S., and Sarhan, F., Cloning, characterization, and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat. Plant Physiol., 99, 1381-1387 (1992).
5. Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K., and Shinozaki, K., Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotechnology, 17, 287-291 (1999).
6. Nanjo, T., Kobayahi, M., Yoshiba, Y., Kakubari, Y., Yamaguchi-Shinozaki, K., and Shinozaki, K., Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana. FEBS Letter, 461, 205-210 (1999).
7. Jaglo-Ottosen, K. R., Gilmour, S. J., Zarka, D. G., Schabenberger, O., and Thomashow, M. F., Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science, 280, 104-106 (1998).
8. Hatano, S., Sadakane, H., Tutumi, M., and Watanabe, T., Studies on frost hardiness in Chlorella ellipsoidea I. Development of frost hardiness of Chlorella ellipsoidea in synchronous culture. Plant Cell Physiol., 17, 451-457 (1976).
9. Joh, T., Honjoh, K., Yoshimoto, M., Funabashi, J., Miyamoto, T., and Hatano, S., Molecular cloning and expression of hardening-induced genes in Chlorella vulgaris C-27, the most abundant clone encodes a late embryogenesis abundant protein. Plant Cell Physiol., 36, 85-93 (1995).
10. Dure, L. III, Developmental biochemistry of cottonseed embryogenesis and germination: changing mRNA populations as shown in vitro and in vivo protein synthesis. Biochemistry, 20, 4162-4168 (1981).
11. Dure, L. III, The LEA proteins of higher plants. In "Control of Plant Gene Expression1, ed. Verma, D.P.S., CRC Press, Boca Raton, FL. pp. 325-335 (1992).
12. Baker, J., Steele, C., and Dure, L. III, Sequence and characterization of 6 LEA proteins and their genes from cotton. Plant Mol. Biol., 11, 277-291 (1988).
13. Dure, L. III, Crouch, M., Harada, J., Ho, T.-H. D., Mundy, J., Quantrano, R., Thomas, T., and Sung, Z. R., Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol. Biol., 12, 475-486 (1989).
14. Dure, L. III , A repeating 11-mer amino acid motif and plant desiccation. Plant J., 3, 363-369 (1993).
15. Skriver, K. and Mundy, J., Gene expression in response to abscisic acid and osmotic stress. Plant Cell, 2, 503-512 (1990).
16. Chandler, P. M. and Robertson, M., Gene expression regulated by abscisic acid and its relation to stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol., 45, 113-141 (1994).
17. Honjoh, K., Oda, Y., Takata, R., Miyamoto, T., and Hatano, S., Introduction of the hiCo6 gene, which encodes a homologue of a late embryogenesis abundant (LEA) protein, enhances freezing tolerance of yeast. J. Plant Physiol., 155, 509-512 (1999).
18. Xu, D., Duan, X., Wang, B., Hong, B., Ho, T.-H. D., and Wu, R., Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol., 110, 249-257 (1986).
19. Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15, 473-497 (1962).
20. Mitsuhara, I., Ugaki, M., Hirochika, H., Ohshima, M., Murakami, T., Gotoh, Y., Katayose, Y., Nakamura, S., Honkura, R., Nishimiya, S., Ueno, K., Mochizuki, A., Tanimoto, H., Tsugawa, H., Otsuki, Y., and Ohashi, Y., Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol., 37, 49-59 (1996).
21. Bevan, M., Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res., 12, 8711-8721 (1984).
22. Rogers, S. G., Horsch, R. B., and Fraley, R. T., Gene transfer in plants: production of transformed plants using Ti plasmid vectors. Methods Enzymol., 118, 627-640 (1986).
23. Lowry, O. H., Rosebrough, N, J., Farr, A.L., and Randall, R. J., Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951)
24. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 221, 680-685 (1970).
25. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., Current Protocols in Molecular Biology. Green Publishing Associates and Wiley-Interscience, New York, pp. 10.8.1-10.8.17 (1987).
26. Shaw, C. H., Plant Molecular Biology - A Practical Approach. Oxford University Press, Oxford, p.207 (1988).
27. Lång, V., Heino, P., and Palva, E. T., Low temperature acclimation and treatment with exogenous abscisic acid induce common polypeptides in Arabidopsis thaliana (L.) Heynh. Theor. Appl. Genet., 77, 729-734 (1989).
28. Webb, M. S., Uemura, M., and Steponkus, P. L., A comparison of freezing injury in oat and rye: two cereals at the extremes of freezing tolerance. Plant Physiol., 104, 467-478 (1994).
29. Tolbert, N. E., Oeser, A., Kisaki, T., Hageman, R. H., and Yamazaki, R. K., Peroxisomes from spinach leaves containing enzymes related to glycolate metabolism. J. Biol. Chem., 243, 5179-5184 (1968).
30. Arnon, D. I., Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta Vulbaris. Plant Physiol., 24, 1-15 (1949).
31. Lin, C., Guo, W. W., Everson, E., and Thomashow, M. F., Cold acclimation in Arabidopsis and wheat: a response associate with expression of regulated genes encoding 'boiling-stable' polypeptides. Plant Physiol., 94, 1078-1083 (1990).
32. Honjoh, K., Yoshimoto, M., Joh, T., Kajiwara, T., Miyamoto, T., and Hatano, S. Isolation and characterization of hardening-induced proteins in Chlorella vulgaris C-27: identification of late embryogenesis abundant proteins. Plant Cell Physiol., 36, 1421-1430 (1995).
33. Steponkus, P. L., Uemura, M., and Webb, M. S., A contrast of the cryostability of the plasma membrane of winter rye and spring oat-two species that widely differ in their freezing tolerance and plasma membrane lipid composition. In "Advances in Low-Temperature Biology", ed. Steponkus P. L., Vol. 2 JAI Press, London, pp. 211-312 (1993).
34. Artus, N. N., Uemura, M., Steponkus, P. L., Gilmour, S. J., Lin, C., and Thomashow, M. F., Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance. Proc. Natl. Acad. Sci. USA, 93, 13404-13409 (1996).
35. Steponkus, P. L., Uemura, M., Joseph, R. A., Gilmour, S. J., and Thomashow, M. F., Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA, 95, 14570-14575 (1998).