Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance

Science

Volumn 280, Issue 5360, 1998, Pages 104-106

  Jaglo Ottosen, Kirsten R ^a   Gilmour, Sarah J ^a   Zarka, Daniel G ^a   Schabenberger, Oliver ^a   Thomashow, Michael F ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; COLD ACCLIMATIZATION; FREEZING; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; THERMODYNAMICS;

ACCLIMATIZATION; ARABIDOPSIS; ARABIDOPSIS PROTEINS; COLD; DNA-BINDING PROTEINS; FREEZING; GENE EXPRESSION REGULATION, PLANT; GENE TRANSFER TECHNIQUES; GENES, PLANT; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS (GENETICS); REGULON; TRANS-ACTIVATORS;

ARABIDOPSIS; EMBRYOPHYTA;

EID: 0032478635     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5360.104     Document Type: Article

References (32)

1. C. L. Guy, Annu. Rev. Plant Physiol. Plant Mol. Biol. 41, 187 (1990); M. A. Hughes and M. A. Dunn, J. Exp. Bot. 47, 291 (1996).
2. C. L. Guy, Annu. Rev. Plant Physiol. Plant Mol. Biol. 41, 187 (1990); M. A. Hughes and M. A. Dunn, J. Exp. Bot. 47, 291 (1996).
3. M. F. Thomashow, in Arabidopsis, E. Meyerowitz and C. Somerville, Eds. (Cold Spring Harbor Laboratory Press, Plainview, NY, 1994), pp. 807-834.
4. S. S. Mohapatra, L. Wolfraim, R. J. Pools, R. S. Dhindsa, Plant Physiol. 89, 375 (1989); M. Houde, R. S. Dhindsa, F. Sarhan, Mol. Gen. Genet. 234, 43 (1992); C. Crosatti, E. Nevo, A. M. Stanca, L. Cattivelli, Theor. Appl. Genet. 93, 975 (1996).
5. S. S. Mohapatra, L. Wolfraim, R. J. Pools, R. S. Dhindsa, Plant Physiol. 89, 375 (1989); M. Houde, R. S. Dhindsa, F. Sarhan, Mol. Gen. Genet. 234, 43 (1992); C. Crosatti, E. Nevo, A. M. Stanca, L. Cattivelli, Theor. Appl. Genet. 93, 975 (1996).
6. S. S. Mohapatra, L. Wolfraim, R. J. Pools, R. S. Dhindsa, Plant Physiol. 89, 375 (1989); M. Houde, R. S. Dhindsa, F. Sarhan, Mol. Gen. Genet. 234, 43 (1992); C. Crosatti, E. Nevo, A. M. Stanca, L. Cattivelli, Theor. Appl. Genet. 93, 975 (1996).
7. N. N. Artus et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13404 (1996).
8. S. J. Gilmour and K. R. Jaglo-Ottosen, unpublished results.
9. M. F. Thomashow, Adv. Genet. 28, 99 (1990).
10. R. K. Hajela, D. P. Horvath, S. J. Gilmour, M. F. Thomashow, Plant Physiol. 93, 1246 (1990).
11. E. J. Stockinger, S. J. Gilmour, M. F Thomashow, Proc. Natl. Acad. Sci. U.S.A. 94, 1035 (1997).
12. K. Yamaguchi-Shinozaki and K. Shinozaki, Plant Cell 6, 251 (1994).
13. S. S. Baker, K. S. Wilhelm, M. F. Thomashow, Plant Mol. Biol. 24, 701 (1994).
14. K. Nordin, T. Vahala, E. T. Palva, ibid. 21, 641 (1993).
15. H. W. Wang, R. Datla, F. Georges, M. Loewen, A. Cutler, ibid. 28, 605 (1995).
16. Standard procedures were used for plasmid manipulations (18). The CBF1-containing Ase I-Bgl II fragment from pACT-Bgl+ (8) was gel purified, Bam HI linkers were ligated to both ends, and the fragment was inserted into the Bam HI site in pCIB710 [S. Rothstein et al., Gene 53, 153 (1987)], which contains the CaMV 35S RNA promoter and terminator. The chimeric plasmid was linearized at the Kpn I site and inserted into the Kpn I site of the binary vector pCIB10g (Ciba-Geigy, Research Triangle Park, NC). The plasmid was transformed into Agrobacterium tumefaciens strain C58C1(pMP90) by electroporation. Arabidopsis plants were transformed by the vacuum infiltration procedure [N. Bechtold, J. Ellis, G. Pelletier, C. R Acad. Sci. Ser. III Life Sci. 316, 1194 (1993)] as modified [A. van Hoof and P. J. Green, Plant J. 10, 415 (1996)].
17. Standard procedures were used for plasmid manipulations (18). The CBF1-containing Ase I-Bgl II fragment from pACT-Bgl+ (8) was gel purified, Bam HI linkers were ligated to both ends, and the fragment was inserted into the Bam HI site in pCIB710 [S. Rothstein et al., Gene 53, 153 (1987)], which contains the CaMV 35S RNA promoter and terminator. The chimeric plasmid was linearized at the Kpn I site and inserted into the Kpn I site of the binary vector pCIB10g (Ciba-Geigy, Research Triangle Park, NC). The plasmid was transformed into Agrobacterium tumefaciens strain C58C1(pMP90) by electroporation. Arabidopsis plants were transformed by the vacuum infiltration procedure [N. Bechtold, J. Ellis, G. Pelletier, C. R Acad. Sci. Ser. III Life Sci. 316, 1194 (1993)] as modified [A. van Hoof and P. J. Green, Plant J. 10, 415 (1996)].
18. Standard procedures were used for plasmid manipulations (18). The CBF1-containing Ase I-Bgl II fragment from pACT-Bgl+ (8) was gel purified, Bam HI linkers were ligated to both ends, and the fragment was inserted into the Bam HI site in pCIB710 [S. Rothstein et al., Gene 53, 153 (1987)], which contains the CaMV 35S RNA promoter and terminator. The chimeric plasmid was linearized at the Kpn I site and inserted into the Kpn I site of the binary vector pCIB10g (Ciba-Geigy, Research Triangle Park, NC). The plasmid was transformed into Agrobacterium tumefaciens strain C58C1(pMP90) by electroporation. Arabidopsis plants were transformed by the vacuum infiltration procedure [N. Bechtold, J. Ellis, G. Pelletier, C. R Acad. Sci. Ser. III Life Sci. 316, 1194 (1993)] as modified [A. van Hoof and P. J. Green, Plant J. 10, 415 (1996)].
19. A. M. Metz, R. T. Timmer, K. S. Browning, Gene 120, 313 (1992).
20. N. P. Sukumaran and C. J. Weiser, HortScience 7, 467 (1972).
21. W. Larcher, in Physiological Processes Limiting Plant Productivity, C. D. Johnson, Ed. (Butterworths, London, 1981), pp. 253-269; W. Larcher and H. Bauer, in Physiological Plant Ecology I. Encyclopedia of Plant Physiology, O. L. Lang, P. S. Nobel, C. B. Osmond, H. Ziegler, Eds. (Springer, Berlin, 1981), vol. 12, pp. 403-437.
22. W. Larcher, in Physiological Processes Limiting Plant Productivity, C. D. Johnson, Ed. (Butterworths, London, 1981), pp. 253-269; W. Larcher and H. Bauer, in Physiological Plant Ecology I. Encyclopedia of Plant Physiology, O. L. Lang, P. S. Nobel, C. B. Osmond, H. Ziegler, Eds. (Springer, Berlin, 1981), vol. 12, pp. 403-437.
23. C. Jiang, B. Iu, J. Singh, Plant Mol. Biol. 30, 679 (1996).
24. J. Sambrook, E. F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, ed. 2, 1989).
25. S. J. Gilmour, R. K. Hajela, M. F. Thomashow, Plant Physiol. 87, 735 (1988).
26. -1) for various amounts of time.
27. 32P by random priming according to standard procedures (18).
28. Total soluble protein was isolated from plant leaves, fractionated by tricine SDS-polyacrylamide gel electrophoresis, and transferred to 0.2-μm nitrocellulose as described (4). COR15am protein was detected with antiserum raised to purified COR15am and protein A-conjugated alkaline phosphatase (Sigma) (4).
29. Electrolyte leakage tests were conducted as described (15, 19) with the following modifications. Two to four detached leaves from nonacclimated or cold-acclimated plants were placed in a test tube and submerged for 1 hour in a -2°C bath containing water and ethylene glycol in a completely randomized design, after which ice crystals were added to nucleate freezing. After an additional hour of incubation at -2°C, the samples were cooled in decrements of 1°C each hour. Samples (five replicates for each data point) were thawed overnight on ice and incubated in 3 ml of distilled water with shaking at room temperature for 3 hours. Electrolyte leakage from leaves was measured with a conductivity meter. The solution was then removed, the leaves were frozen at -80°C (for at least 1 hour), and the solution was returned to each tube and incubated for 3 hours to obtain a value for 100% electrolyte leakage.
30. Pots (9 cm) containing about 40 nonacclimated Arabidopsis plants (20 days old) and 4-day cold-acclimated plants (25 days old) (20) were placed in a completely randomized design in a -5°C cold chamber in the dark. After 1 hour, ice chips were added to each pot to nucleate freezing. Plants were removed after 2 days and returned to a growth chamber at 22°C.
31. 50 values for each plant type, was determined by using SAS PROC GLM [SAS Institute, SAS/STAT User's Guide, Version 6 (SAS Institute, Cary, NC, 1989)].
32. We wish to thank J. Dodgson, B. Sears, T. Deits, and E. Stockinger for critical reading of the manuscript. This research was supported in part by grants to M.F.T. from the National Science Foundation (IBN 9307348), the U.S. Department of Agriculture/National Research Initiative Competitive Grants Program (96-35100-3231), and the Michigan Agricultural Experiment Station.