Expression of clpX, an ATPase subunit of the Clp protease, is heat and cold shock inducible in Lactococcus lactis

Journal of Dairy Science

Volumn 84, Issue 8, 2001, Pages 1783-1785

  Skinner, M M ^a   Trempy, J E ^a  

^a OREGON STATE UNIVERSITY   (United States)

Author keywords

ATPase; ClpX; Cold shock; Heat shock; Lactococcus lactis; Protease

Indexed keywords

LACTOCOCCUS LACTIS;

ADENOSINE TRIPHOSPHATASE; BACTERIAL PROTEIN; CHAPERONE; CLPX PROTEIN, E COLI; ENDOPEPTIDASE CLP; ESCHERICHIA COLI PROTEIN; HEAT SHOCK PROTEIN; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; COLD; CRYOPRESERVATION; GENE EXPRESSION REGULATION; GENETICS; HEAT; LACTOCOCCUS LACTIS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; COLD; CRYOPRESERVATION; ENDOPEPTIDASE CLP; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; HEAT; HEAT-SHOCK PROTEINS; LACTOCOCCUS LACTIS; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; SERINE ENDOPEPTIDASES;

EID: 0035432287     PISSN: 00220302     EISSN: None     Source Type: Journal    
DOI: 10.3168/jds.S0022-0302(01)74615-2     Document Type: Article

References (15)

1. Broadbent, J. R., and C. Lin. 1999. Effect of heat shock or cold shock treatment on the resistance of Lactococcus lactis to freezing and lyophilization. Cryobiology 39:88-102.
2. Frees, D., and H. Ingmer. 1999. ClpP participates in the degradation of misfolded protein in Lactococcus lactis. Mol. Microbiol. 31:79-87.
3. Gerth, U., A. Wipat, C. R. Harwood, N. Carter, P. T. Emmerson, and M. Hecker. 1996. Sequence and transcriptional analysis of clpX, a class-III heat-shock gene of Bacillus subtilis. Gene 181:77-83.
4. Gottesman, S., W. P. Clark, V. de Crecy-Lagard, and M. R. Maurizi. 1993. ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli. Sequence and in vivo activities. J. Biol. Chem. 268:22618-22626.
5. Harrison, R. W., J. C. Miller, M. J. D'Souza, and G. Kampo. 1997. Easy gene walking. Biotechniques 22:650-653.
6. Hwang B. J., K. M. Woo, A. L. Goldberg, and C. H. Chung. 1988. Protease Ti, a new ATP-dependent protease in Escherichia coli, contains protein-activated ATPase and proteolytic functions in distinct subunits. J. Biol. Chem. 263:8727-8734.
7. Ingmer, H., F. K. Vogensen, K. Hammer, and M. Kilstrup. 1999. Disruption and analysis of the clpB, clpC, and clpE genes in Lactococcus lactis: ClpE, a new Clp family in gram-positive bacteria. J. Bacteriol. 181:2075-2083.
8. Jobin, M. P., D. Garmyn, C. Divies, and J. Guzzo. 1999. The Oenococcus oeni clpX homologue is a heat shock gene preferentially expressed in exponential growth phase. J. Bacteriol. 181:6634-6641.
9. Maurizi, M. R., W. P. Clark, S. H. Kim, and S. Gottesman. 1990. ClpP represents a unique family of serine proteases. J. Biol. Chem. 265:12546-12552.
10. Schirmer, E. C., J. R Glover, M. A Singer, and S. Lindquist. 1996. HSP100/Clp proteins: a common mechanism explains diverse functions. Trends Biochem. Sci. 21:289-296.
11. Wawrzynow, A., D. Wojtkowiak, J. Marszalek, B. Banecki, M. Jonsen, B. Graves, C. Georgopoulos, and M. Zylicz. 1995. The ClpX heat-shock protein of Escherichia coli, the ATP-dependent substrate specificity component of the ClpP-ClpX protease, is a novel molecular chaperone. EMBO J. 14:1867-1877.
12. Wickner, S., S. Gottesman, D. Skowyra, J. Hoskins, K. McKenney, and M. R. Maurizi. 1994. A molecular chaperone, ClpA, functions like DnaK and DnaJ. Proc. Natl. Acad. Sci. USA 91:12218-12222.
13. Woo, K. M., W. J. Chung, D. B. Ha, A. L. Goldberg, and C. H. Chung. 1989. Protease Ti from Escherichia coli requires ATP hydrolysis for protein breakdown but not for hydrolysis of small peptides. J. Biol. Chem. 264:2088-2091.
14. Wouters, J. A., B. Jeynov, F. M. Rombouts, W. M. de Vos, O. P. Kuipers, and T. Abee. 1999. Analysis of the role of 7 kDa cold-shock proteins of Lactococcus lactis MG1363 in cryoprotection. Microbiology 145:3185-3194.
15. Wouters, J. A., J-W. Sanders, J. Kok, W. M. de Vos, O. P. Kuipers, and T. Abee. 1998. Clustered organization and transcriptional analysis of a family of five csp genes of Lactococcus lactis MG1363. Microbiology 144:2885-2893.