Cysteine residues of SNAP-25 are required for SNARE disassembly and exocytosis, but not for membrane targeting

Biochemical Journal

Volumn 357, Issue 3, 2001, Pages 625-634

  Washbourne, P ^a   Cansino, V ^a   Mathews, J R ^a   Graham, M ^a   Burgoyne, R D ^a   Wilson, M C ^a  

^a UNIVERSITY OF NEW MEXICO SCHOOL OF MEDICINE   (United States)

Author keywords

N ethylmaleimide sensitive fusion protein; Palmitoylation; Syntaxin; Vesicle fusion; Vesicle associated membrane protein

Indexed keywords

LIPIDS; MUTAGENS; OPTIMIZATION; PROTEINS;

NEUROTRANSMITTER;

BIOCHEMISTRY;

CYSTEINE; HYBRID PROTEIN; MEMBRANE PROTEIN; N ETHYLMALEIMIDE; NEUROTRANSMITTER; SNARE PROTEIN; SYNAPTOBREVIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNTAXIN;

ANIMAL CELL; ARTICLE; EXOCYTOSIS; GOLGI COMPLEX; LIPID BILAYER; MEMBRANE TRANSPORT; NEUROTRANSMITTER RELEASE; NONHUMAN; PALMITOYLATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; SYNAPSE; SYNAPSE VESICLE;

ANIMALS; ANTIGENS, SURFACE; CARRIER PROTEINS; CELLS, CULTURED; CRICETINAE; CYSTEINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; EXOCYTOSIS; MEMBRANE PROTEINS; NERVE TISSUE PROTEINS; PC12 CELLS; PROTEIN STRUCTURE, TERTIARY; RATS; SNARE PROTEINS; SUBCELLULAR FRACTIONS; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; SYNTAXIN 1; TRANSFECTION; VESICULAR TRANSPORT PROTEINS;

EID: 0035425203     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3570625     Document Type: Article

References (49)

1. SNAP receptors implicated in vesicle targeting and fusion
2. SNAP 25, a T SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils
3. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
4. Implications of the SNARE hypothesis for intracellular membrane topology and dynamics
5. SNARE interactions are not selective
6. SNAREs contribute to the specificity of membrane fusion
7. SNAREpins: Minimal machinery for membrane fusion
8. Rapid and efficient fusion of phospholipid vesicles by the alpha-helical core of a SNARE complex in the absence of an N-terminal regulatory domain
9. 2+ and drives membrane fusion
10. The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulation
11. rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin
12. Visualization of the dynamics of synaptic vesicle and plasma membrane proteins in living axons
13. Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane
14. Human cDNA clones encoding two different isoforms of the nerve terminal protein SNAP-25
15. The 25 kDa synaptosomal-associated protein SNAP-25 is the major methionine-rich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS
16. Characterization of the palmitoylation domain of SNAP-25
17. SNAP-23 and SNAP-25 are palmitoylated in vivo
18. Regulated vesicular fusion in neurons: Snapping together the details
19. Multiple palmitoylation of synaptotagmin and the T SNARE SNAP-25
20. SNAP-25 palmitoylation and plasma membrane targeting require a functional secretory pathway
21. SNAP-25 is targeted to the plasma membrane through a novel membrane-binding domain
22. Botulinum neurotoxin E-insensitive mutants of SNAP-25 fail to bind VAMP but support exocytosis
23. N-ethylmaleimide-sensitive fusion protein: A trimeric ATPase whose hydrolysis of ATP is required for membrane fusion
24. Structural determinants of the specificity for synaptic vesicle associated membrane protein/synaptobrevin of tetanus and botulinum type B and G neurotoxins
25. A simple enzymatic method for the preparation of radiolabeled erucoyl-CoA and other long-chain fatty acyl-CoAs and their characterization by mass spectrometry
26. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion
27. Targeting of SNAP-25 to membranes is mediated by its association with the target SNARE syntaxin
28. Blockade of membrane transport and disassembly of the Golgi complex by expression of syntaxin 1A in neurosecretion incompetent cells: Prevention by rbSEC1
29. A novel 58-kDa protein associates with the Golgi apparatus and microtubules
30. Synaptic vesicle membrane fusion complex: Action of clostridial neurotoxins on assembly
31. Neurotransmission: Harnessing fusion machinery at the synapse
32. Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E
33. Proteolysis of SNAP-25 by types E and A botulinal neurotoxins
34. Rapid anterograde axonal transport of the syntaxin-SNAP 25-VAMP complex
35. A membrane-targeting signal in the amino terminus of the neuronal protein GAP-43
36. All ras proteins are polyisoprenylated but only some are palmitoylated
37. A novel N-terminal motif for palmitoylation of G-protein α-subunits
38. Overview: Protein palmitoylation in the nervous system: Current views and unsolved problems
39. Fatty acylation of proteins: New insights into membrane targeting of myristoylated and palmitoylated proteins
40. Palmitoylation of the 25-kDa synaptosomal protein (SNAP-25) in vitro occurs in the absence an enzyme, but is stimulated by binding to syntaxin
41. Differential localization of syntaxin isoforms in polarized Madin-Darby canine kidney cells
42. The major 35S-methionine-labeled rapidly transported protein (superprotein) is identical to SNAP-25, a protein of synaptic terminals
43. SNAP-25 is present on the Golgi apparatus of retinal neurons
44. Clostridial neurotoxins compromise the stability of a low energy SNARE complex mediating NSF activation of synaptic vesicle fusion
45. Fatty acylation promotes fusion of transport vesicles with Golgi cisternae
46. Synaptobrevin 2 is palmitoylated in synaptic vesicles prepared from adult, but not from embryonic brain
47. SNAREpins are functionally resistant to disruption by NSF and alpha-SNAP
48. Membrane localization and biological activity of SNAP-25 cysteine mutants in insulin-secreting cells
49. The pathway of membrane fusion catalyzed by influenza hemagglutinin: Restriction of lipids, hemifusion, and lipidic fusion pore formation