In vitro model using mouse hepatocytes for study of alcohol stress

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 7, 2001, Pages 1528-1533

  Yang, Jae Won ^a   Shin, Jun Seop ^a   Lee, Jae Jeong ^a   Chang, Hyo Ihl ^a   Kim, Chan Wha ^a  

^a Korea University   (South Korea)

Author keywords

Allyl alcohol; CYP2E1; Ethanol; Hepatotoxicity; Mouse hepatocyte

Indexed keywords

ALCOHOL; ALLYL ALCOHOL; AMMONIA; CYTOCHROME P450 2E1; INSULIN; PROPANOL; XENOBIOTIC AGENT;

ANIMAL; ARTICLE; BIOLOGICAL MODEL; CELL SURVIVAL; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; IN VITRO STUDY; INSTITUTE FOR CANCER RESEARCH MOUSE; LIVER CELL; METABOLISM; MOUSE;

AMMONIA; ANIMALS; CELL SURVIVAL; CYTOCHROME P-450 CYP2E1; DOSE-RESPONSE RELATIONSHIP, DRUG; ETHANOL; HEPATOCYTES; INSULIN; MICE; MICE, INBRED ICR; MODELS, BIOLOGICAL; PROPANOLS; XENOBIOTICS;

EID: 0035403453     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1528     Document Type: Article

References (17)

1. Tsutsumi, M., Lasker, J. M., Shimizu, M., Rosman, A. S., and Lieber, C. S., The intralobular distribution of ethanol-inducible P450IIEI in rat and human liver. Hepatology, 10, 437-446 (1989).
2. Louis, C. A., Wood, S. G., Kostrubsky, V., Sinclair, P. R., and Sinclair, J. F., Synergistic increases in rat hepatic cytochrome P450s by ethanol and isopentanol. J. Pharmacol. Exp. Ther., 269, 838-845 (1994).
3. Koop, D. R., Klopfenstein, B., Iimuro, Y. and Thurman, R. G., Gadolinium chloride blocks alcohol-dependent liver toxicity in rats treated chronically with intragastric alcohol despite the induction of CYP2E1. Mol. Pharmacol., 51, 944-950 (1997).
4. Woodcroft, K. J., Novak, R. F., Xenobiotic-enhanced expression of Cytochromes P450 2E1 and 2B in primary cultured rat hepatocytes. Drug. Metab. Dispos., 26, 372-378 (1998).
5. Seglen, P. G., Preparation of rat liver cells: III Enzymatic requirement for tissue dispersion. Exp. Cell. Res., 82, 391-398 (1973).
6. Bader, A., Knop, E. Kern, A., Boker, K., Fruhauf, N., Crome, O., Esselmann, H., Pape, C., Kempka, G., and Sewing, K. F., 3-D cocuture of Hepatic sinusoidal cells with primary hepatocytes - Design of an organotypical model. Exp. Cell. Res., 215, 332-337. (1996).
7. Koebe, H. G. and Schildberg, F. W., Isolation of porcine hepatocytes from slaughterhouse organs. Int. J. Artif. Organs., 19, 53-60 (1996).
8. Denizat, F. and Lang, R., Rapid colorimetric assay for cell growth and survival. J. Immunol. Methods., 89, 271-277 (1986).
9. Xu, Y., Jones, B. E., Neufeld, D. S., and Czaja, M. J., Gluthathion modulates rat and mouse hepatocytes sensitivity tumor necrosis factor a toxicity. Gastroenterotogy, 115, 1229-1237 (1998).
10. Bergmeyer, H. U., Bernt, E. and Hess, B., Lactic dehydriogenase. In Methods in enzymatic analysis, eds. Bergmeyer, H. U., Verlag Chemie, Wheinheim, Germany, pp. 736-741 (1984).
11. Sambrook, J., Fritsch, E. F., and Maniatis, T., SDS-polyacrylamide gel electrophoresis of proteins, In Molecular Cloning: A laboratory manual, eds. Sambrook, J., Fritsch, E. F., and Maniatis, T., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp18.47-18.75 (1989).
12. Pang, J., Zaleski, J., and Kauffman, F. C., Action of selected hepatotoxicants on freshly isolated cryopreserved rat hepatocytes. Cryobiology., 33, 226-235 (1996).
13. Sakurai, J., Funae, Y. and Nemoto, N., Maintenance and activation of Cyp2e-1 gene expression in mouse hepatocytes in primary culture. Biochim. Biophys. Acta, 1313, 35-40 (1996).
14. Peng, H. M. and Coon, M. J., Regulation of rabbit cytochrome P450 2E1 expression in HepG2 cells by insulin and thyroid hormon. Mol. Pharmacol., 54, 740-747 (1998).
15. Carpenter, S. P., Lasker, J. M., and Raucy, J. L., Expression, induction, and catalytic activity of the ethanol-inducible cytochrome P450 (CYP2E1) in human fetal liver and hepatocytes. Mol. Pharmacol., 49, 260-268 (1996).
16. Yoshida, Y., Kimura, N. Oda, H. and Kakinuma, A., Insulin suppresses the induction of CYP2B1 and CYP2B2 gene expression by phenobarbital in adult rat cultured hepatocytes. Biochem. Biophys. Res. Commun., 229, 182-188 (1996).
17. Woodcroft, K. J., Novak, R. F., Insulin differentially affects xenobiotic-enhanced, cytochrome P-450 (CYP)2E1, CYP2B, CYP3A, and CYP4A expression in primary cultured rat hepatocytes. J. Pharmacol. Exp. Ther., 289, 1121-1127 (1999).