Chromosomal Gene Inactivation in the Green Sulfur Bacterium Chlorobium tepidum by Natural Transformation

Applied and Environmental Microbiology

Volumn 67, Issue 6, 2001, Pages 2538-2544

  Frigaard, Niels Ulrik ^a   Bryant, Donald A ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIUM TRANSFORMATION; CHLOROBI; GENE SILENCING; GENETIC MARKER; GENETIC RECOMBINATION; GENETIC SELECTION; GENETICS; NITROGEN FIXATION;

CHLOROBI; CHROMOSOMES, BACTERIAL; DRUG RESISTANCE, MICROBIAL; GENE SILENCING; GENES, BACTERIAL; GENETIC MARKERS; NITROGEN FIXATION; RECOMBINATION, GENETIC; SELECTION (GENETICS); TRANSFORMATION, BACTERIAL;

EID: 0035377523     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.6.2538-2544.2001     Document Type: Article

References (24)

1. Becker, A., M. Schmidt, W. Jäger, and A. Pühler. 1995. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Gene 162:37-39.
2. Blankenship, R. E., M. T. Madigan, and C. E. Bauer. 1995. Anoxygenic photosynthetic bacteria. Kluwer Academic Publishers. Dordrecht. The Netherlands.
3. Castenholz, R. W., J. Bauld, and B. B. Jørgensen. 1990. Anoxygenic microbial mats of hot springs: thermophilic Chlorobium sp. FEMS Microbiol. Ecol. 74:325-336.
4. Chung, S., G. Frank, H. Zuber, and D. A. Bryant. 1994. Genes encoding two chlorosome components from the green sulfur bacteria Chlorobium vibrioforme strain 8327D and Chlorobium lepidum. Photosynth. Res. 41:261-275.
5. Chung, S., G. Shen, J. Ormerod, and D. A. Bryant. 1998. Insertional inactivation studies of the csmA and csmC genes of the green sulfur bacterium Chlorobium vibrioforme 8327: the chlorosome protein CsmA is required for viability but CsmC is dispensable. FEMS Microhiol. Lett. 164:353-361.
6. Collis, C. M., M.-J. Kim, H. W. Stokes, and R. M. Hall. 1998. Binding of the purified integron DNA integrase IntII to integron- and cassette-associated recombination sites. Mol. Microbiol. 29:477-490.
7. Elhai, J., and C. P. Wolk. 1988. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119-138.
8. Gruber, T. M., and D. A. Bryant. 1998. Characterization of the group 1 and group 2 sigma factors of the green sulfur bacterium Chlorobium tepidum and the green nonsulfur bacterium Chloroflexus aurantiacus. Arch. Microbiol. 170:285-296.
9. Kjærulff, S., D. B. Diep, J. S. Okkels, H. V. Scheller, and J. G. Ormerod. 1994. Highly efficient integration of foreign DNA into the genome of the green sulfur bacterium Chlorobium vibrioforme by homologous recombination. Photosynth. Res. 41:277-283.
10. Méndez-Alvarez, S., V. Pavón, I. Esteve, R. Guerrero, and N. Gaju. 1994. Transformation of Chlorobium limicola by a plasmid that confers the ability to utilize thiosulfate. J. Bacteriol. 176:7395-7397.
11. Nogales, B., R. Guerrero, and I. Esteve. 1994. Susceptibility of various purple and green sulfur bacteria to different antimicrobial agents. FEMS Microbiol. Lett. 123:37-42.
12. Ormerod, J. G. 1988. Natural genetic transformation in Chlorobium, p. 315-319. In J. M. Olson, J. G. Ormerod, J. Amesz, E. Stackebrandt, and H. G. Trüper (ed.), Green photosynthetic bacteria. Plenum Press, New York, N.Y.
13. Overmann, J., and C. Tuschak. 1997. Phylogeny and molecular fingerprinting of green sulfur bacteria. Arch. Microbiol. 167:302-309.
14. Palmen, R., and K. J. Hellingwerf. 1997. Uptake and processing of DNA by Acinetobacter calcoaceticus - a review. Gene 192:179-190.
15. Postgate, J. 1998. Nitrogen fixation. 3rd ed. Cambridge University Press, Cambridge, United Kingdom.
16. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
17. Prentki, P., A. Binda, and A. Epstein. 1991. Plasmid vectors for selecting ISI-promoted deletions in cloned DNA: sequence analysis of the omega interposon. Gene 103:17-23.
18. Recchia, G. D., and R. M. Hall. 1995. Gene cassettes: a new class of mobile element. Microbiology 141:3015-3027.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Thiel, T. 1994. Genetic analysis of cyanobacteria, p. 581-611. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.
21. Trüper, H. G, and N. Pfennig. 1992. The family Chlorobiaceae, p. 3583-3592. In A. Balows, H. G. Trüper, M. Dworkin, W. Harder, and K.-H. Schleifer (ed.), The prokaryotes, 2nd ed. Springer-Verlag, New York, N.Y.
22. Wahlund, T. M., C. R. Woese, R. W. Castenholz, and M. T. Madigan. 1991. A thermophilic green sulfur bacterium from New Zealand hot springs. Chlorobium tepidum sp. nov. Arch. Microbiol. 156:81-90.
23. Wahlund, T. M., and M. T. Madigan. 1993. Nitrogen fixation by the thermophilic green sulfur bacterium Chlorobium tepidum. J. Bactcriol. 175:474-478.
24. Wahlund, T. M., and M. T. Madigan. 1995. Genetic transfer by conjugation in the thermophilic green sulfur bacterium Chlorobium tepidum. J. Bacteriol. 177:2583-2588.