Use of Leu3a/3b for the accurate determination of CD4 subsets for measurement of intracellular cytokines

Cytometry

Volumn 44, Issue 2, 2001, Pages 148-152

  Styles, J A ^a   Clark, H ^a   Festing, M F W ^a   Rew, D A ^a,b  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY HOSPITAL SOUTHAMPTON NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

CD4 subsets; Cytokines; Human; T cells

Indexed keywords

BREFELDIN A; CALCIUM IONOPHORE; CD3 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; LEUCINE; OKT 4; PHORBOL 13 ACETATE 12 MYRISTATE;

ACCURACY; ARTICLE; CELL CULTURE; CELL SELECTION; CELL STIMULATION; CONTROLLED STUDY; CYTOKINE RELEASE; FLOW CYTOMETRY; HELPER CELL; HUMAN; HUMAN CELL; INCUBATION TIME; INTERMETHOD COMPARISON; INTERNALIZATION; MEASUREMENT; NORMAL HUMAN; PRIORITY JOURNAL; PRODUCTIVITY; QUANTITATIVE ASSAY; SIGNAL NOISE RATIO; T LYMPHOCYTE SUBPOPULATION;

ANTIGENS, CD4; CD4-POSITIVE T-LYMPHOCYTES; FLOW CYTOMETRY; HUMANS; INTERFERON TYPE II; INTERLEUKIN-4; INTRACELLULAR FLUID;

EID: 0035371273     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20010601)44:2<148::AID-CYTO1094>3.0.CO;2-6     Document Type: Article

References (6)

1. Analysis of Th1 and Th2 cytokine production by peripheral blood mononuclear cells as a parameter of immunological dysfunction in advanced cancer patients
2. Internalization, lysosomal degradation and new synthesis of surface membrane CD4 in phorbol ester-activated T-lymphocytes and U-937 cells
3. Expression and kinetics of cytokines determined by intracellular staining using flow cytometry
4. Detection of intracellular cytokines by flow cytometry
5. Determination of intracellular cytokines by flow cytometry following whole-blood culture
6. Detecting cytokine production at the single cell level