Genetic manipulation of spirochetes - Light at the end of the tunnel

Trends in Microbiology

Volumn 9, Issue 6, 2001, Pages 245-248

  Cabello, Felipe C ^a   Sartakova, Marina L ^a   Dobrikova, Elena Yu ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL; COUMAMYCIN; ERYTHROMYCIN; KANAMYCIN; LEPTOTENE; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

BORRELIA BURGDORFERI; BORRELIA INFECTION; BRACHYSPIRA; BRACHYSPIRA HYODYSENTERIAE; BREVINEMA; CLONING VECTOR; CONTROLLED STUDY; CRISTISPIRA; ELECTROPORATION; ENZYME INHIBITION; GENE ISOLATION; GENETIC COMPLEMENTATION; GENETIC MANIPULATION; GENETIC MARKER; LEPTOSPIRA; LEPTOSPIRA BIFLEXA; LEPTOSPIROSIS; LYME DISEASE; MOLECULAR GENETICS; MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SHORT SURVEY; SPIROCHAETA; SPIROCHETE; SYPHILIS; TREPONEMA DENTICOLA; TREPONEMA PALLIDUM;

ANIMALIA; BORRELIA; BORRELIA BURGDORFERI; BRACHYSPIRA; BRACHYSPIRA HYODYSENTERIAE; BREVINEMA; CRISTISPIRA; LEPTOSPIRA; LEPTOSPIRA BIFLEXA; SPIROCHAETA; SPIROCHAETALES; TREPONEMA DENTICOLA; TREPONEMA PALLIDUM;

EID: 0035370095     PISSN: 0966842X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0966-842X(01)02046-7     Document Type: Review

References (24)

1. B.J Paster F.E Dewhirst Phylogenetic foundation of spirochetes J. Mol. Microbiol. Biotechnol. 2 2000 341 344
2. C.M Fraser Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi Nature 390 1997 580 586
3. C.M Fraser Complete genome sequence of Treponema pallidum , the syphilis spirochete Science 281 1998 375 388
4. G Subramanian Comparative genomic analysis of the pathogenic spirochetes Borrelia burgdorferi and Treponema pallidum Infect. Immun. 68 2000 1633 1648
5. R.L Zuerner Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity J. Bacteriol. 175 1993 5445 5451
6. S Falkow Molecular Koch's postulate applied to microbial pathogenicity Rev. Infect. Dis. 10 Suppl. 2 1998 S274 S276
7. K Tilly DNA exchange and insertional inactivation in spirochetes J. Mol. Microbiol. Biotechnol. 2 2000 433 442
8. M Sartakova Development of an extrachromosomal cloning vector system for use in Borrelia burgdorferi Proc. Natl. Acad. Sci. USA 97 2000 4850 4855
9. A.A.H.M ter Huurne Inactivation of a Serpula (Treponema) hyodysenteriae hemolysin gene by homologous recombination: importance of this hemolysin in pathogenesis of S. hyodysenteriae in mice FEMS Microbiol. Lett. 92 1992 109 114
10. E.L Rosey Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange J. Bacteriol. 177 1995 5959 5970
11. E.J Rosey Dual flaA1 flaB1 mutant of Serpulina hyodysenteriae expressing periplasmic flagella is severely attenuated in a murine model of swine dysentery Infect. Immun. 64 1996 4154 4162
12. S.B Humphrey Purification and characterization of VSH-1, a generalized transducing bacteriophage of Serpulina hyodysenteriae J. Bacteriol. 179 1997 323 329
13. H Li H.K Kuramitsu Development of a gene transfer system in Treponema denticola by electroporation Oral Microbiol. Immunol. 11 1996 161 165
14. H Li Gene inactivation in the oral spirochete Treponema denticola : construction of an flgE mutant J. Bacteriol. 178 1996 3664 3667
15. I Saint Girons Development of shuttle vectors for spirochetes J. Mol. Microbiol. Biotechnol. 2 2000 443 445
16. D.S Samuels Genetic transformation of the Lyme disease agent Borrelia burgdorferi with coumarin-resistant gyrB J. Bacteriol. 176 1994 6045 6049
17. A.F Elias Altered stationary-phase response in a Borrelia burgdorferi rpoS mutant J. Bacteriol. 182 2000 2909 2918
18. J.L Bono Efficient targeted mutagenesis in Borrelia burgdorferi J. Bacteriol. 182 2000 2445 2452
19. M.A Motaleb Borrelia burgdorferi periplasmic flagella have both skeletal and motility functions Proc. Natl. Acad. Sci. USA 97 2000 10899 10904
20. P.E Stewart Isolation of a circular plasmid region sufficient for autonomous replication and transformation of infectious Borrelia burgdorferi Mol. Microbiol. 39 2001 714 721
21. I Saint Girons The LE1 bacteriophage replicates as a plasmid within Leptospira biflexa : construction of an L. biflexa–Escherichia coli shuttle vector J. Bacteriol. 182 2000 5700 5705
22. M Picardeau First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella Mol. Microbiol. 40 2001 189 199
23. S.L Chiang In vivo genetic analysis of bacterial virulence Annu. Rev. Microbiol. 53 1999 129 154
24. J Izard Cytoplasmic filament-deficient mutant of Treponema denticola has pleiotropic defects J. Bacteriol. 183 2001 1078 1084