Isoenzyme Multiplicity and Characterization of Recombinant Manganese Peroxidases from Ceriporiopsis subvermispora and Phanerochaete chrysosporium

Applied and Environmental Microbiology

Volumn 67, Issue 5, 2001, Pages 2070-2075

  Larrondo, Luis F ^a   Lobos, Sergio ^b   Stewart, Phillip ^c   Cullen, Dan ^c   Vicuña, Rafael ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^b UNIVERSITY OF CHILE   (Chile)

^c FOREST PRODUCTS LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ISOENZYME; MANGANESE; MANGANESE PEROXIDASE; OXALIC ACID DERIVATIVE; PEROXIDASE; RECOMBINANT PROTEIN;

ARTICLE; ASPERGILLUS NIDULANS; BASIDIOMYCETES; ENZYME ACTIVATION; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PHANEROCHAETE;

ASPERGILLUS NIDULANS; BASIDIOMYCOTA; ENZYME ACTIVATION; GLYCOSYLATION; ISOENZYMES; KINETICS; MANGANESE; OXALATES; PEROXIDASES; PHANEROCHAETE; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

EID: 0035347318     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.5.2070-2075.2001     Document Type: Article

References (30)

1. Ballance, D. J., F. P. Buxton, and G. Turner. 1983. Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa. Biochem. Biophys. Res. Commun. 112:284-289.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Cullen, D., G. L. Gray, L. J. Wilson, K. J. Hayenga, M. H. Lamsa, M. W. Rey, S. Norton, and R. M. Berka. 1987. Controlled expression and secretion of bovine chymosin in Aspergillus nidulans. Bio/Technology 5:369-375.
4. Cullen, D. 1997. Recent advances on the molecular genetics of ligninolytic fungi. J. Biotechnol. 53:273-289.
5. Glenn, J. K., and M. H. Gold. 1985. Purification and characterization of an extracellular Mn(II) dependent peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Arch. Biochem. Biophys. 242:329-341.
6. Horton, R. M., H. D. Hunt, S. N. Ho, J. K. Pullen, and L. R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68.
7. Karahanian, E., G. Corsini, S. Lobos, and R. Vicuña. 1998. Structure and expression of a laccase gene from the ligninolytic basidiomycete Ceriporiopsis subvermispora. Biochim. Biophys. Acta 1443:65-74.
8. Kern, G., N. Schulke, F. X. Schmid, and R. Jaenicke. 1992. Stability, quaternary structure, and folding of internal, external, and core-glycosylated invertase from yeast. Protein Sci. 1:120-131.
9. Kersten, P. J., C. Witek, A. van den Wymelenberg, and D. Cullen. 1995. Phanerochaete chrysosporium glyoxal oxidase is encoded by two allelic variants: structure, genomic organization, and heterologous expression of glx1 and glx2. J. Bacteriol 177:6106-6110.
10. Kuan, I. C., and M. Tien. 1989. Phosphorylation of lignin peroxidase from Phanerochaete chrymsporium. J. Biol. Chem. 264:20350-20355.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
12. Lobos, S., J. Larraín, L. Salas, D. Cullen, and R. Vicuña. 1994. Isoenzymes of manganese-dependent peroxidase and lacease produced by the lignindegrading basidiomycete Ceriporiopsis subvemispora. Microbiology 140: 2691-2698.
13. Lobos, S., L. Larrondo, L. Salas, E. Karahanian, and K. Vicuña. 1998. Cloning and molecular analysis of a cDNA and the Cs-mnpl gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete Ceriporiopsis subvemispora. Gene 206:185-193.
14. Nie, G., N. S. Reading, and S. D. Aust. 1999. Relative stability of recombinant versus native peroxidases from Phanerochaete chrysosporium. Arch. Biochem. Biophys. 365:328-334.
15. Oakley, B. R., J. E. Rinehart, B. L. Mitchell, C. E. Oakley, C. Carmona, G. L. Gray, and G. S. May. 1987 Cloning, mapping and molecular analysis of the pyrG (orotidine-5′-phosphate decarboxylase) gene of Aspergillus nidulans. Gene 61:385-399.
16. Paszcynski, A., V. B Huynh, and R. L. Crawford. 1985. Enzymatic activities of a extracellular, manganese-dependent peroxidase from Phanerochaete chrysosporium. FEMS Microbiol. Lett. 27:37-41.
17. Pease, E., S. D. Aust, and M. Tien. 1991. Heterologous expression of active manganese peroxidase from Phanerochaete chrysosporium using the baculovirus expression system. Biochem. Biophys. Res. Commun. 179:897-903.
18. Pease, E., and M. Tien. 1992. Heterogeneity and regulation of manganese peroxidases from Phanerochaete chrysosporium. J. Bacteriol. 174:3532-3540.
19. 2+ and different aromatic substrates. Appl. Environ. Microbiol. 65:4705-4707.
20. Sarath, G., R. S. de la Motte, and F. W. Wagner. 1989. Protease assay methods, p. 25-55. In R. J. Beynon and J. S. Bond (ed.), Proteolytic enzymes: a practical approach. IRL Press, Oxford, United Kingdom.
21. Stewart, P., R. E. Whitwam, P. J. Kersten, D. Cullen, and M. Tien. 1996. Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. Appl. Environ. Microbiol. 62:860-864.
22. Tani, S., T. Kawaguchi, M. Kato, T. Kobayashi, and N. Tsukagoshi. 2000. A novel nuclear factor, SREB, binds to a cis-acting element, SRE, required for inducible expression of the Aspergillus oryzae Taka-amylase A gene in A. nidulans. Mol. Gen. Genet. 263:232-238.
23. Tello, M., G. Corsini, L. F. Larrondo, L. Salas, S. Lobos, and R. Vicuña. 2000. Characterization of three new manganese peroxidase genes from the ligninolytic basidiomycete Ceriporiopsis subvermispora. Biochim. Biophys. Acta 1490:137-144.
24. Urzúa, L., L. E. Larrondo, S. Lobos, J. Larrain, and R. Vicuña. 1995. Oxidation reactions catalyzed by manganese peroxidase isoenzymes from Ceriporiopsis subvermispora. FEBS Lett. 371:132-136.
25. von Heijne, G. 1985. Signal sequences. The limits of variation. J. Mol. Biol. 184:99-105.
26. Wallis, G. L. F., R. J. Swift, F. W. Hemming, A. P. J. Trinci, and J. F. Peberdy. 1999. Glucoamylase overexpression and secretion in Aspergillus niger: analysis of glycosylation. Biochim. Biophys. Acta 1472:576-586.
27. Wang, C., M. Eufemi, C. Turano, and A. Giartosio. 1996. Influence of the carbohydrate moiety on the stability of glycoproteins. Biochemistry 35:7299-7307.
28. Wariishi, H., L. Akileswaran, and M. H. Gold. 1988. Manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: spectral characterization of the oxidized states and the catalytic cycle. Biochemistry 27:5365-5370.
29. Wariishi, H., H. B. Dunford, I. D. MacDonald, and M. H. Gold. 1989. Manganese peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium: transient-state kinetics and reaction mechanism. J. Biol. Chem. 264:23688-23695.
30. Wyss, M., L. Pasamontes, A. Friedlein, R. Rémy, M. Tessier, A. Kronenberger, A. Middendorf, M. Lehmann, L. Schnoehelen, U. Röthlisberger, E. Kusznir, G. Wahl, F. Müller, H. W. Lahm, K. Vogel, and A. P. G. M. van Loon. 1999. Biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance. Appl. Environ. Microbiol. 65:359-366.