Purification and Characterization of Ginsenoside Rb1-Metabolizing β-Glucosidase from Fusobacterium K-60, a Human Intestinal Anaerobic Bacterium

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 5, 2001, Pages 1163-1169

  Park, Sun Young ^a   Bae, Eun Ah ^a   Sung, Jong Hwan ^b   Lee, Seung Kwon ^b   Kim, Dong Hyun ^a  

^a Kyung Hee University   (South Korea)

^b IL HWA Co Ltd   (South Korea)

Author keywords

glucosidase; Fusobacterium K 60; Ginsenoside Rb1 transforming activity; IH 901

Indexed keywords

BETA GLUCOSIDASE; GINSENOSIDE; SAPONIN;

ARTICLE; ENZYME SPECIFICITY; ENZYMOLOGY; FUSOBACTERIUM; HUMAN; HYDROLYSIS; INTESTINE; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY;

BETA-GLUCOSIDASE; FUSOBACTERIUM; GINSENOSIDES; HUMANS; HYDROLYSIS; INTESTINES; SAPONINS; SUBSTRATE SPECIFICITY;

EID: 0035346143     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1163     Document Type: Article

References (22)

1. Trease, G. E. and Evans, W. C., Ginseng. In "Pharmacognosy" Bailliere Tindall, London, pp. 45-456 (1983).
2. Nagai, M., Ando S., Tanaka, N., Tanaka, O., and Shibata, S., Chemical studies on the Oriental plant drugs. XXVIII. Saponins and sapogenins of ginseng: stereochemistry of the sapogenin of ginsenoside Rb1, Rb2 and Rc. Chem. Pharm. Bull., 20, 1212-1216 (1972).
3. Karikura, M., Miyase, T., Tanizawa, H., Tanizawa, T., and Takino, Y., Studies on absorption, distribution, excretion and metabolism of ginseng saponins. VII. Comparison of the decomposition modes of ginsenoside-Rb1 and -Rb2 in the digestive tract of rats. Chem. Pharm. Bull., 39, 2357-2361 (1991).
4. Wu, J. Y., Gardner, B. H., Murphy, C. I., Seals, J. R., Kensil, C. R., Recchia, J., Beltz, G. A., and Newman, G. W., Newman M. J., Saponin adjuvant enhancement of antigen-specific immune responses to an experimental HIV-1 vaccine. J. Immunol., 148, 1519-1525 (1992).
5. Sato, K., Mochizuki, M., Saiki, I., Yoo, Y. C., Samukawa, K., and Azuma, I., Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng, ginsenoside-Rb2. Biol. Pharm. Bull., 17, 635-639 (1994).
6. Mochizuki, M., Yoo, Y. C., Matsuzawa, K., Sato, K., Saiki, I., Tono-oka, S., Samukawa, K., and Azuma, I., Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside-Rb2, 20(R)- and 20(S)-ginsenoside-Rg3, of red ginseng. Biol. Pharm. Bull., 18, 1197-1202 (1995).
7. Karikura, M., Miyase, T., Tanizawa, H., Tanizawa, T., and Takino, Y., Studies on absorption, distribution, excretion and metabolism of ginseng saponins. VI. The decomposition products of ginsenoside Rb2 in the stomach of rats. Chem. Pharm. Bull., 39, 400-404 (1991).
8. Kanaoka, M, Kato, H, Shimada, F., and Yano, S., Studies on the enzyme immunoassay of bio-active constituents in oriental medicinal drugs. VI. Enzyme immunoassay of ginsenoside Rb1 from Panax ginseng. Chem. Pharm. Bull., 40, 314-317 (1992).
9. Kanaoka, M., Akao, T., and Kobashi, K., Metabolism of ginseng saponins, ginsenosides, by human intestinal flora. J. Tradit. Med., 11, 241-245 (1994).
10. Akao, T., Akao, T., and Kobashi, K., Appearance of compound K, a major metabolite of ginsenoside Rb1 by intestinal bacteria, in rat plasma after oral administration-measurement of compound K by enzyme immunoassay. Biol. Pharm. Bull., 21, 245-249 (1998).
11. Akao, T., Kida, H., Kanaoka, M., Hattori, M., and Kobashi, K., Intestinal bacterial hydrolysis is required for the appearance of compound K in rat plasma after oral administration of ginsenoside Rb1 from Panax ginseng. J. Pharm. Pharmacol., 50, 1155-1160 (1998).
12. Wakabayashi, C., Hasegawa, H., Murata, J., and Saiki, I., In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol. Res., 9, 411-417 (1997).
13. Lee, S. J., Sung, J. H., Lee, S. J., Moon, C. K., and Lee, B. H., Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin. Cancer Lett., 144, 39-43 (1999).
14. Hasegawa, H., Sung, J. H., Matsumiya, S., and Uchiyama, M., Main ginseng saponin metabolites formed by intestinal bacteria. Planta Med., 62, 453-457 (1996).
15. Hasegawa, H., Sung, J-W., and Benno, Y., Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins. Planta Medica, 63, 436-440 (1997).
16. Hasegawa H., Lee K.-S., Nagaoka T., Tezuka Y., Uchiyama M., Kadota S., and Saiki I., Pharmacokinetics of ginsenoside deglycosylated by intestinal bacteria and its transformation to biologically active fatty acid esters. Biol. Pharm. Bull., 23, 298-304 (2000).
17. Moore, W. E. C., Holdeman, L., and Kelley, R. W., Genus II. Fusobacterium Knorr. In "Bergey's manual of systemic bacteriology" eds by Krieg N. R. and Holt, J. G., Williams and Wilkins, Baltimore, pp. 631-637 (1984).
18. Dahkqvist A., Assay of intestinal disaccharidases. Enzymol. Biol. Clin., 11, 52-56 (1970).
19. Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 72, 248-254 (1976).
20. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature, 227, 680-685 (1970).
21. Kim, D.-H., Sohng, I.-S., Kobashi, K., and Han, M. J., Purification and characterization of β-glucosidase from Bacteroides JY-6, a human intestinal bacterium. Biol. Pharm. Bull., 19, 1121-1125 (1996).
22. Yang, L., Akao, T., and Kobashi, K., Purification and characterization of a geniposide-hydrolyzing β-glucosidase from Eubacterium sp. A-44, a strict anaerobe from human feces. Biol. Pharm. Bull., 18, 1184-1188 (1995).