Skin is an autonomous organ in synthesis, two-step activation and degradation of vitamin D3: CYP27 in epidermis completes the set of essential vitamin D3-hydroxylases

Steroids

Volumn 66, Issue 3-5, 2001, Pages 399-408

  Schuessler, Monika ^a   Astecker, Norbert ^a,b   Herzig, Gerda ^a   Vorisek, Georg ^a   Schuster, Inge ^a,c  

^a Research Institute of the Vienna   (Austria)

^b WOMEN AND INFANTS HOSPITAL   (United States)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

25 Hydroxylase; 25 Hydroxyvitamin D3; CYP27; Keratinocytes; Steroid; Vitamin D3

Indexed keywords

24,25 DIHYDROXYCOLECALCIFEROL; CALCIDIOL 1 MONOOXYGENASE; CALCIFEDIOL; CALCITRIOL; COLECALCIFEROL; CYTOCHROME P450 ISOENZYME; MESSENGER RNA; STEROID MONOOXYGENASE; TRITIUM;

ARTICLE; BIODEGRADATION; CELL CULTURE; CONTROLLED STUDY; ENZYME KINETICS; EPIDERMIS; GENE EXPRESSION; HOMEOSTASIS; HUMAN; HUMAN CELL; HUMAN TISSUE; HYDROXYLATION; KERATINOCYTE; LIVER; METABOLIC ACTIVATION; METABOLITE; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN; SKIN DISEASE; TIME; VITAMIN D METABOLISM;

CALCITRIOL; CELLS, CULTURED; CHOLECALCIFEROL; CYTOCHROME P-450 CYP27A1; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION REGULATION; HUMANS; KERATINOCYTES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SKIN; STEROID HYDROXYLASES;

EID: 0035342730     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0039-128X(00)00229-4     Document Type: Article

References (41)

1. 3; a unique photoendocrine system
2. Vitamin D; the vitamin and the hormone
3. Vitamin D; recent advances
4. Mechanisms, and functions of vitamin D
5. Current understanding of the molecular actions of vitamin D
6. 3
7. 3 production by human keratinocytes
8. Cloning of human 25-hydroxyvitamin D-1α-hydroxylase and mutations causing vitamin D-dependent rickets type 1
9. Vitamin D hydroxylases
10. 3 receptors in human skin biopsies
11. 3
12. 3 receptors in normal and psoriatic skin
13. 3 on the morphologic and biochemical differentiation of cultured human epidermal keratinocytes grown in serum free conditions
14. 3
15. Vitamin D, calcium, and epidermal differentiation
16. Biological activity of vitamin D analogues in the skin, with special reference to antipsoriatic mechanisms
17. 3 and skin diseases
18. 3-24-hydroxylase mRNA in cultured human keratinocytes
19. 3-24-hydroxylase, a multicatalytic enzyme
20. Selective inhibition of vitamin D-hydroxylases in human keratinocytes
21. 3 by perfused pig skin
22. 3
23. 3 (calcitriol)
24. A rapid method of total lipid extraction and purification
25. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
26. A novel method for real time quantitative RT-PCR
27. Uptake of sulfate conjugates by isolated rat hepatocytes
28. 3
29. Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis
30. Complete structure of the human gene for the vitamin D-1-α-hydroxylase, P450c1alpha
31. 3-24-hydroxylase cDNA
32. Direct quantification of picomolar concentrations of mRNAs by mathematical analysis of a reverse transcription/exponential polymerase chain reaction (PCR) assay
33. Efficient synthesis of double dye-labeled oligodeoxyribonucleotide probes and their application in a real time PCR assay
34. Selective inhibitors of CYP24; Mechanistic tools to explore vitamin D-metabolism in human keratinocytes
35. Quantitation of the main metabolites of vitamin D in a single serum sample, II. Determination by UV-absorption and competitive protein binding assays
36. Low density lipoprotein (LDL) cholesterol is converted to 27.hydroxycholesterol in human fibroblasts
37. 3 production