SRβ coordinates signal sequence release from SRP with ribosome binding to the translocon

EMBO Journal

Volumn 20, Issue 9, 2001, Pages 2338-2347

  Fulga, Tudor A ^a   Sinning, Irmgard ^b   Dobberstein, Bernhard ^b   Pool, Martin R ^b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Endoplasmic reticulum; GTPase; Protein translocation; Ribosome; Signal recognition particle receptor (SR)

Indexed keywords

DOCKING PROTEIN; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; RIBOSOME PROTEIN; SIGNAL RECOGNITION PARTICLE; TRANSLOCON; UNCLASSIFIED DRUG; CELL RECEPTOR; MEMBRANE PROTEIN; PROTEIN SUBUNIT; RECEPTOR; RECOMBINANT PROTEIN; SEC61 PROTEIN; SIGNAL PEPTIDE; SIGNAL PEPTIDE RECEPTOR; SRP72 PROTEIN, MOUSE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DOG; ENDOPLASMIC RETICULUM; MICROSOME; NONHUMAN; PANCREAS; PRIORITY JOURNAL; PROTEIN TARGETING; RIBOSOME; ANIMAL; CELL FREE SYSTEM; GENETICS; HUMAN; METABOLISM; MOUSE; PHYSIOLOGY; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT; SITE DIRECTED MUTAGENESIS;

ANIMALIA; CANIS FAMILIARIS;

ANIMALS; CELL-FREE SYSTEM; DOGS; GTP PHOSPHOHYDROLASES; GUANOSINE TRIPHOSPHATE; HUMANS; MEMBRANE PROTEINS; MICE; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; PROTEIN TRANSPORT; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, PEPTIDE; RECOMBINANT PROTEINS; RIBOSOMAL PROTEINS; RIBOSOMES; SIGNAL RECOGNITION PARTICLE;

EID: 0035341399     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.9.2338     Document Type: Article

References (46)

1. Evidence for a two-step mechanism involved in assembly of functional signal recognition particle receptor
2. Regulation by the ribosome of the GTPase of the signal recognition particle during protein targeting
3. The ribosome regulates the GTPase of the β-subunit of the signal recognition particle receptor
4. Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex
5. Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle
6. The GTPase superfamily: Conserved structure and molecular mechanism
7. Formation of a functional ribosome-membrane junction during translocation requires the participation of a GTP-binding protein
8. Access of proteinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes
9. The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation
10. Anionic phospholipids are involved in membrane association of FtsY and stimulate its GTPase activity
11. Structure of the conserved GTPase domain of the signal recognition particle
12. Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor
13. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane
14. A mammalian homolog of Sec61p and SecYp is associated with ribosomes and nascent polypeptides during translocation
15. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation
16. A complex comprising the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins
17. Site-specific photocross-linking reveals that Sec61p and TRAM contact different regions of a membrane inserted signal sequence
18. The human docking protein does not associate with the membrane of the rough endoplasmic reticulum via a signal or insertion sequence-mediated mechanism
19. The translocon: A dynamic gateway at the ER membrane
20. Binding of ribosomes to the rough endoplasmic reticulum mediated by the Sec61p-complex
21. Nucleotide-dependent binding of the GTPase domain of the signal recognition particle receptor β-subunit to the α-subunit
22. Both lumenal and cytosolic gating of the aqueous ER translocon pore is regulated from within the ribosome during membrane protein integration
23. The protein conducting channel in the membrane of the endoplasmic reticulum is open laterally toward the lipid bilayer
24. A membrane component essential for vectorial translocation of nascent proteins across the endoplasmic reticulum: Requirements for its extraction and reassociation with the membrane
25. Characterization of molecules involved in protein translocation using a specific antibody
26. Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor
27. The β subunit of the signal recognition particle receptor is a transmembrane GTPase that anchors the α subunit, a peripheral membrane GTPase, to the endoplasmic reticulum membrane
28. Crystal structure of the NG domain from the signal recognition particle receptor FtsY
29. The signal recognition particle receptor of Escherichia coli (FtsY) has a nucleotide exchange factor built into the GTPase domain
30. Binding of signal recognition particle gives ribosome/nascent chain complexes a competitive advantage in endoplasmic reticulum membrane interaction
31. A functional GTPase domain, but not its transmembrane domain, is required for function of the SRP receptor β-subunit
32. Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases
33. Signal recognition particle-dependent targeting of ribosomes to the rough endoplasmic reticulum in the absence and presence of the nascent polypeptide-associated complex
34. Protein translocation across the ER requires a functional GTP binding site in the α subunit of the signal recognition particle receptor
35. Empty site forms of SRP54 and SRα GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum
36. Kinetics and thermodynamics of the interaction of elongation factor Tu with factor Ts, guanine nucleotides and aminoacyl-tRNA
37. Homology of 54K protein of signal-recognition particle, docking protein and two E.coli proteins with putative GTP-binding domains
38. Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis
39. SecA is not required for signal recognition particle-mediated targeting and initial membrane insertion of a nascent inner membrane protein
40. A protein-conducting channel in the endoplasmic reticulum
41. Role of Sec61α in the regulated transfer of the ribosome-nascent chain complex from the signal recognition particle to the translocation channel
42. The signal recognition particle receptor is a complex that contains two distinct polypeptide chains
43. Preparation of microsomal membranes for cotranslational protein translocation
44. Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane
45. Toward a model for the interaction between elongation factor Tu and the ribosome