Characterization of membrane-localized and cytosolic Rac-GTPase-activating proteins in human neutrophil granulocytes: Contribution to the regulation of NADPH oxidase

Biochemical Journal

Volumn 355, Issue 3, 2001, Pages 851-858

  Gelszt, Miklós ^a   Dagher, Marie Claire ^b   Molnár, Gergely ^a   Havasi, Andrea ^a   Faure, Julien ^b   Paclet, Marie Hélène ^c   Morel, Francoise ^c   Ligeti, Erzsébet ^a  

^a SEMMELWEIS UNIVERSITY   (Hungary)

^b CEA GRENOBLE   (France)

^c GRENOBLE UNIVERSITY HOSPITAL   (France)

Author keywords

Overlay; Phagocytes; Prenylation

Indexed keywords

ANTIBODIES; BIOASSAY; BIOLOGICAL MEMBRANES; CELLS;

INTRACELLULAR LOCALIZATION;

PROTEINS;

GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; GUANOSINE TRIPHOSPHATE; PHOSPHORUS 32; RAC PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; CELL MEMBRANE; CYTOSOL; ENZYME ACTIVATION; GRANULOCYTE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOPRECIPITATION; NEUTROPHIL; PHAGOCYTE; PRIORITY JOURNAL;

EID: 0035339971     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/bj3550851     Document Type: Article

References (54)

1. 1 Bourne, H. R., Sanders, D. A. and McCormick, F. (1990) The GTPase superfamily: a conserved switch far diverse cell functions. Nature (London) 348, 125-131
2. 2 Van Aelst, L. and D'Souza-Schorey, C. (1997) Rho GTPases and signaling networks. Genes Dev. 11, 2295-2322
3. 3 Hall, A. (1998) Rho GTPases and the actin cytoskeleton. Science 279,509-514
4. 4 Symons, M. and Settleman, J. (2000) Rho family GTPases: more than simple switches. Trends Cell Biol. 10, 415-419
5. 5 Qui, R. G., Chen, J., Kim, D., McCormick, F. and Symons, M. (1995) An essential role for Rac in Ras transformation. Nature (London) 374, 457-459
6. 6 Minden, A., Lin, A., Claret, F. X., Abo, A. and Karin, M. (1995) Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs. Cell 81, 1147-1157
7. 7 Hill, C. S., Wynne, J. and Treisman, R. (1995) The Rho family GTPases RhoA, Rac1, and CDC42HS regulate transcriptional activation by SRF. Cell 81, 1159-1170
8. 8 Vojtek, A. B. and Cooper, J. A. (1995) Rho family members: activators of MAP kinase cascades. Cell 82, 527-529
9. 9 Abo, A., Pick, E., Hall, A., Totty, N., Teahan, C. G. and Segai, A. W. (1991) Activation of the NADPH oxidase involves the small GTP-binding protein p21rac1. Nature (London) 353, 668-670
10. 10 Morel, F., Doussiere, J. and Vignais, P. V. (1991) The superoxide-generating oxidase of phagocytic cells. Physiological, molecular and pathological aspects. Eur. J. Biochem. 201, 523-546
11. 11 Segal, A. W. and Abo, A. (1993) The biochemical basis of the NADPH oxidase of phagocytes. Trends Biochem. Sci. 18, 43-47
12. 12 Babior, B. M. (1999) NADPH oxidase: an update. Blood 93, 1464-1476
13. phox and regulation of phagocytic NADPH oxidase activity. Science 265, 531-533
14. 14 Dorseuil, O., Relbel, L., Bokoch, G. M. Camonis, J. and Gacon, G. (1996) The Rac target NADPH oxidase p67phox interacts preferentially with Rac2 rather than Rac1. J. Biol. Chem. 271, 83-88
15. 15 Leusen, J. H., de Klein, A., Hilarius, P. M., Ahlin, A., Palmblad, J., Smith, C. I., Diekmann, D., Hall, A., Vertioeven, A. J. and Roos, D. (1996) Disturbed interaction of p21-rac with mutated p67-phox causes chronic granulomatous disease. J. Exp. Med. 184, 1243-1249
16. 16 Ding, J. and Badwey, J. A. (1993) Neutrophils stimulated with a chemotactic peptide or a phorbol ester exhibit different alterations in the activities of a battery of protein kinases. J. Biol. Chem. 268, 5234-5240
17. 17 Manser, E., Leung, T., Salihuddin, H., Zhao, Z. S. and Lim, L. (1994) A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature (London) 367, 40-46
18. 18 Knaus, U. G., Morris, S., Dong, H. J., Cherhoff, J. and Bokoch, G. M. (1995) Regulation of human leukocyte p21-activated kinases through G protein-coupled receptors. Science 269, 221-223
19. 19 Ding, J., Knaus, U. G., Lian, J. P., Bokoch, G. M. and Badwey, J. A. (1996) The renaturable 69- and 63-kDa protein kinases that undergo rapid activation in chemoattractant-stimulated guinea pig neutrophils are p21-activated kinases. J. Biol. Chem. 271, 24869-24873
20. 20 Bokoch, G. M., Vlahos, C. J., Wang, Y., Knaus, U. G. and Traynor-Kaplan, A. E. (1996) Rac GTPase interacts specifically with phosphatidylinositol 3-kinase. Biochem. J. 315, 775-779
21. 21 Keety, P. J., Westwick, J. K., Whitehead, I. P., Der, C. J. and Parise, L. V. (1997) Cdc42 and Rac1 induce integrin-medlated cell motility and invasiveness through P1(3)K. Nature (London) 390, 632-636
22. 22 Arcaro, A. and Wymann, M. P. (1993) Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phospnatidylinositol 3,4,5-triphosphate in neutrophil responses. Biochem. J. 296, 297-301
23. 23 Okada, T., Sakuma, L. Fukui, Y., Hazeki, O. and Ui, M. (1994) Blockage of chemotactic peptide-induced stimulation ot neutropnils by wortmannin as a result of selective inhibition of phosphatidylinositol 3-kinase. J. Biol. Chem. 269, 3563-3567
24. 24 Hirsch, E., Katanaev, V. L., Garianda, C., Azzolino, O., Pirola, L., Silengo, L., Sozzanl, S., Mantovani, A., Altruda, F. and Wymann, M. P. (2000) Central role for G protein-coupled phosphoinositide 3-kinase γ in inflammation. Science 287,1049-1053
25. 25 Li, Z., Jiang, H., Xie, W., Zhang, Z., Smrcka, A. V. and Wu, D. (2000) Roles of PLC-β2and β3 and P13K-γ in chemoattractant-mediated signal transduction. Science 287, 1046-1049
26. 26 Han, J., Luby-Phelps, K., Das, B., Shu, X., Xia, Y., Mosteller, R. D., Krishna, U. M., Falck, J. R., White, M. A. and Broek, D. (1998) Role of substrates and products of Pl 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav. Science 279, 558-560
27. 27 Boguski, M. S. and McCormick, F. (1993) Proteins regulating Ras and its relatives. Nature (London) 366, 643-654
28. 28 Wolff, J., Dagher, M. C., Fuchs, A., Gelszt, M. and Ligeti, E. (1996) In vitro activation of the NADPH oxidase by fluoride. Possible involvement of a factor activating GTP hydrolysis on Rac (Rac-GAP). Eur. J. Biochem. 239, 369-375
29. 29 Seifert, R., Rosenthal, W. and Schultz, G. (1986) Guanine nucleotides stimulate NADPH oxidase in membranes of human neutrophils. FEBS Lett. 205, 161-165
30. 30 Gabig, T. G., English, D., Akard, L. P. and Schell, M. J. (1987) Regulation of neutrophil NADPH oxidase activation in a cell-tree system by guanine nucleotides and fluoride. J. Biol. Chem. 262, 1685-1690
31. --generating oxidase in plasma membrane from bovine polymorphonuclear neutrophils by arachidonic acid, a cytosolic factor of protein nature and nonhydrolyzable analogues of GTP. Biochemistry 27, 193-200
32. 32 Aviram, I. and Sharabani, M. (1989) Kinetics of cell-free activation of neutraphil NADPH oxidase. Biochem. J. 261, 477-482
33. 33 Heyworth, P. G., Knaus, U. G., Settleman, J., Curnutte, J. T. and Bokoch, G. M. (1993) Regulation of NADPH oxidase activity by Rac GTPase activating protein(s). Mol. Biol. Cell 4, 1217-1223
34. 34 Voncken, J. W., van Schalck, H., Kaartinen, V., Deemer, K., Coates, T., Landing, B., Pattengale, P., Dorseuil, O., Bokoch, G. M. and Groffen, J. (1995) Increased neutrophil respiratory burst in bcr-null mutants. Cell 80, 719-728
35. 35 Grandvaux, N., Grizot, S., Vignais, P. V. and Daghet, M. C. (1999) The Ku70 antigen interacts with p40phox in B lymphocytes. J. Cell Sci. 112, 503-513
36. 36 Batot, G., Paciet, M. H., Doussiere, J., Vergnaud, S., Martel, C., Vignais, P. V. and Morel, F. (1998) Biochemical and immunochemical properties of B lymphocyte cytochrome b558. Biochim. Biophys. Acta 1406, 188-202
37. - generating NADPH oxidase in a semi-recombinant cell-free system. Eur. J. Biochem. 226, 587-595
38. 38 Sell, A. and Hall, A. (1995) Purification of recombinant Rho/Rac/G25K from Escherichia coli. Methods Enzymol. 256B, 3-10
39. 39 Hjorth, R., Jonsson, A. K. and Vretblad, P. (1981) A rapid method for purification of human granulocytes using percoll. A comparison with dextran sedimentation. J. Immunol. Methods. 43, 95-101
40. 40 Sell, A. and Hall, A. (1995) Measurement of intrinsic nucleotide exchange and GTP hydrolysis rates. Methods Enzymol. 256B, 67-76
41. 4. Nature (London) 227, 680-685
42. 42 Manser, E., Leung, T. Monfries, C., Teo, M., Hall, C. and Lim, L. (1992) Diversity and versatility of GTPase activating proteins for the p21rho subfamily of ras G proteins detected by a novel overlay assay. J. Biol. Chem. 267, 16025-16028
43. 43 Manser, E., Leung, T. and Lim, L. (1995) Identification of GTPase-activating proteins by nitrocellulose overlay assay. Methods Enzymol. 256B, 130-139
44. 44 Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
45. 45 Hancock, J. F. and Hall, A. (1993) A novel role for RhoGDI as an inhibitor of GAP proteins. EMBO J. 12, 1915-1921
46. 46 Szászi, K., Korda, A., Wolff, J., Paciet, M. H., Morel, F. and Ligeti, E. (1999) Possible role of Rac-GTPase activating protein in the termination of superoxide production in phagocytic cells. Free Radical Biol. Med. 27, 764-772
47. rac Nature (London) 351, 400--402
48. 48 Dusi, S., Donini, M., Wientjes, F. and Rossi, F. (1996) Translocation of p190rho guanosine triphosphatase-activating protein from cytosol to the membrane in human neutrophils stimulated with different agonists. Biochem. Biophys. Res. Commun. 219, 859-862
49. 49 Lancaster, C., Taylor-Harris, P. M., Self, A. J., Brill, S., van Erp, H. E. and Hall, A. (1994) Characterization of rhoGAP. J. Biol. Chem. 269, 1137-1142
50. 50 Hart, M. J., Shinjo, K., Hall, A., Evans, T. and Cerione, R. (1991) Identification of the human platelet GTPase activating protein for the CDC42HS protein. J. Biol. Cnem. 266, 20840-20848
51. 51 Garrett, M. D., Major, G. N., Totty, N. and Hall, A. (1991) Publication and N-terminal sequence of the p21rho GTPase-activating protein, rho GAP. Biochem. J. 276, 833-836
52. 52 Moril, N., Kawano, K., Sekine, A., Yamada, T. and Narumiya, S. (1991) Purification of GTPase-activating protein specific for the Rho gene products. J. Biol. Chem. 266, 7646-7650
53. 53 Barfod, E. T., Zheng, Y., Kuang, W. J., Hart, M. J., Evans, T., Cerione, R. A. and Astikenazi, A. (1993) Cloning and expression of a human CDC42 GTPase-activating protein reveals a functional SH3-binding domain. J. Biol. Chem. 268, 26059-26062
54. 54 Leung, T., How, B. E., Mansert, E. and Lim, L. (1993) Germ cell beta-chimaerin, a new GTPase-activating protein for p21rac, is specifically expressed during the acrosomal assembly stage in rat testis. J. Biol. Chem. 268, 3813-3816