The preparation of rat heme oxygenase-1 mutant to reduce the level of bilirubin

Chinese Medical Journal

Volumn 114, Issue 4, 2001, Pages 348-351

  Xia, Zhenwei ^a   Shao, Jie ^a   Shen, Qingxiang ^b   Wang, Jian ^b   Li, Yunzhu ^a   Chen, Shunnian ^a   Yu, Shanchang ^a  

^a SHANGHAI SECOND MEDICAL UNIVERSITY   (China)

^b SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH   (China)

Author keywords

Heme oxygenase; Hyperbilirubinemia; Mutant

Indexed keywords

BILIRUBIN; COMPLEMENTARY DNA; HEME OXYGENASE; HEME OXYGENASE 1;

ANIMAL; ARTICLE; CELL STRAIN COS1; DRUG ANTAGONISM; GENETICS; HYPERBILIRUBINEMIA; MOLECULAR WEIGHT; MUTATION; RAT;

ANIMALS; BILIRUBIN; COS CELLS; DNA, COMPLEMENTARY; HEME OXYGENASE (DECYCLIZING); HEME OXYGENASE-1; HYPERBILIRUBINEMIA; MOLECULAR WEIGHT; MUTATION; RATS;

EID: 0035323272     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Ishikawa K, Sato M, Yoshida T. Expression of rat heme oxygenase in Escherichia coli as a catalytically active, full-length form that binds to bacterial membrances. Eur J Biochem 1991; 202:161-165.
2. Xia ZW, Li YZ, Chen SN, et al. The purification and identification of heme oxygenase isoforms from spleen tissue of rat and the expression of heme oxygenase-1 cDNA in COS-1 cells. Chin Med J 1998;111:842-846.
3. Ishikawa K, Sato M, Ito M, et al. Importance of histidine residue 25 of rat heme oxygenase for its catalytic activity Biochem Biophys Res Commun 1992;182:981-986.
4. Liu Y, Moenne-Loccoz P, Hildebrand DP, et al. Replacement of the proximal histidine iron ligand by a cysteine or typosine converts heme oxygenase to an oxidase. Biochemistry 1999;38: 3733-3743.
5. Ito M, Ishikawa K, Matera KM, et al. Demonstration that histindine 25, but not 132, is the axial heme ligand in rat heme oxygenase-1. Arch Biochem Biophys 1995;317:253-258.