Characterization of the active-site residues asparagine 167 and lysine 161 of the IMP-1 metallo β-lactamase

FEMS Microbiology Letters

Volumn 197, Issue 1, 2001, Pages 85-89

  Haruta, Shin ^a,b   Yamamoto, Elise Tie ^a   Eriguchi, Yoshiro ^a   Sawai, Tetsuo ^a  

^a CHIBA UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Lactam antibiotic; Lactam resistance; Metallo lactamase

Indexed keywords

ALANINE; ASPARAGINE; ASPARTIC ACID; BETA LACTAMASE; CARBOXYL GROUP; CEPHALOSPORIN; INOSINE PHOSPHATE; LYSINE; PENICILLIN G;

AMINO ACID SEQUENCE; ARTICLE; ELECTRICITY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; GENE CONSTRUCT; KINETICS; MUTATION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ASPARAGINE; BETA-LACTAMASES; BETA-LACTAMS; BINDING SITES; ESCHERICHIA COLI; HUMANS; KINETICS; LYSINE; MUTAGENESIS, SITE-DIRECTED; ZINC;

EID: 0035313529     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(01)00091-X     Document Type: Article

References (11)

1. Functional analysis of the active-site of a metallo-β-lactamase proliferating in Japan
2. Crystal structure of the IMP-1 metallo β-lactamase from Pseudomonas aeruginosa and its complex with a mercaptocarboxylate inhibitor: Binding determinants of a potent, broad-spectrum inhibitor
3. The crystal structure of the L1 metallo-β-lactamase from Stenotrophomonas maltophilia at 1.7 Å resolution
4. Role of lysine-67 in the active site of class C β-lactamase from Citrobacter freundii GN346
5. Production of single-stranded plasmid DNA
6. An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis
7. Novel metallo β-lactamase mediated by a Shigella flexneri plasmid
8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
9. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
10. Kinetic studies on inactivation of Citrobacter freundii cephalosporinase by sulbactam
11. Crystal structure of the wide-spectrum binuclear zinc β-lactamase from Bacteroides fragilis