Development of duplex PCR assay for rapid detection of enterotoxigenic isolates of Clostridium perfringens

Southeast Asian Journal of Tropical Medicine and Public Health

Volumn 32, Issue 1, 2001, Pages 105-113

  Tansuphasiri, Unchalee ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

ENTEROTOXIN; ENTEROTOXIN, CLOSTRIDIUM; PRIMER DNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; BACTERIAL GENE; BACTERIAL SPORE; CLOSTRIDIUM PERFRINGENS; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BASE SEQUENCE; CLOSTRIDIUM PERFRINGENS; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; ENTEROTOXINS; GENES, BACTERIAL; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPORES, BACTERIAL;

EID: 0035288593     PISSN: 01251562     EISSN: 26975718     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. American Public Health Association. Clostridium perfringens food poisoning. In : Benenson AS, ed. Control of communicable diseases in man. Washington, DC: American Public Health Association, 1985: 148-9.
2. Baez LA, Juneja VK, Sackitey SK. Chemiluminescent enzyme immunoassay for detection of PCR-amplified enterotoxin A from Clostridium perfringens. Int J Food Microbiol 1996; 32: 145-58.
3. Birkhead G, Vogt RL, Heun EM, Snyder JT, McClane BA. Characterization of an outbreak of Clostridium perfringens food poisoning by quantitative fecal culture and fecal enterotoxin measurement. J Clin Microbiol 1988; 26: 471-4.
4. Borriello SP, Welch AR, Stringer MF, Larson HE, Barclay F, Bartholomew BA. Enterotoxigenic Clostridium perfringens : a possible cause of antibiotic-associated diarrhoea. Lancet 1984; 1: 305-7.
5. Canard B, Saint-Joanis B, Cole ST. Genomic diversity and organization of virulence genes in the pathogenic anaerobe Clostridium perfringens. Mol Microbiol 1992; 6: 1421-9.
6. Cato EP, George WL, Finegold SM. Genus Clostridium. In : Sneath P, ed. Bergey's manual of systemic bacteriology, vol 2. Baltimore, Md: Williams and Wilkins, 1986 : 1141-82.
7. Czeczulin JR, Hanna PC, McClane BA. Cloning, nucleotide sequencing, and expression of the Clostridium perfringens enterotoxin gene in Escherichia coli. Infect Immun 1993; 61: 3429-39.
8. Duncan CL, Strong DH, Sebald M. Sporulation and enterotoxin production by mutants of Clostridium perfringens. J Bacteriol 1972; 110: 378-91.
9. Fach P, Guillou JP. Detection by in vitro amplification of the alpha-toxin (phospholipase C) gene from Clostridium perfringens. J Appl Bacteriol 1993; 74: 61-6.
10. Fach P, Popoff MR. Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test. Appl Environ Microbiol 1997; 63: 4232-6.
11. Kokai-Kun JF, Songer JG, Czeczulin JR, Chen F, McClane BA. Comparison of Western immunoblots and gene detection assays for identification of potentially enterotoxigenic isolates of Clostridium perfringens. J Clin Microbiol 1994; 32: 2533-9.
12. McClane BA. An overview of Clostridium perfringens enterotoxin. Toxicon 1996; 34: 1335-43.
13. Mpamugo O, Donovan T, Brett MM. Enterotoxigenic Clostridium perfringens as a cause of sporadic cases of diarrhoea. J Med Microbiol 1995; 43: 442-5.
14. Ridell J, Bjorkroth J, Eisgruber H, Schalch B, Stolle A, Korkeala H. Prevalence of the enterotoxin gene and clonality of Clostridium perfringens strains associated with food-poisoning outbreaks. J Food Protect 1998; 61: 240-3.
15. Saito M, Matsumoto M, Funabashi M. Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure. Int J Food Microbiol 1992; 17: 47-55.
16. Skjelvale R, Uemura T. Experimental diarrhea in human volunteers following oral administration of Clostridium perfringens enterotoxin. J Appl Bacteriol 1977; 43: 281-6.
17. Stringer MF, Turnbull PCB, Gilbert RJ. Application of serological typing to the investigation of outbreaks of Clostridium perfringens food poisoning, 1970-1978. J Hyg 1980; 84: 443-56.
18. Stringer MF, Watson GN, Gilbert RJ, et al. Fecal carriage of Clostridium perfringens. J Hyg 1985; 95: 277-88.
19. Sutton RGA. Enumeration of Clostridium welchii in the faeces of varying section of the human population. J Hyg 1966; 64: 367-74.
20. Titball RW, Hunter SEC, Martin KL, et al. Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens. Infect Immun 1989; 57: 367-76.
21. Tso JY, Siebel C. Cloning and expression of the phospholipase C gene from Clostridium perfringens and Clostridium bifermentans. Infect Immun 1989; 57: 468-76.
22. Uemura T, Yoshitake S, Hu D, Kajikawa T. A highly sensitive enzyme-linked immunosorbent assay for Clostridium perfringens enterotoxin. Lett Appl Microbiol 1992; 15: 23-5.
23. Van Damme-Jongsten M, Wernars K, Notermans S. Cloning and sequencing of the Clostridium perfringens enterotoxin gene. Anton van Leeuwen 1989; 56: 181-90.
24. Van Damme-Jongsten M, Rodhouse MJ, Gilbert RJ, Notermans S. Synthetic DNA probes for detection of enterotoxigenic Clostridium perfringens strains isolated from outbreaks of food poisoning. J Clin Microbiol 1990; 28: 131-3.
25. Yamagishi T, Serikawa T, Morita R, Nakamura S, Nishida S. Persistent high numbers of Clostridium perfringens in the intestines of Japanese aged adults. Jpn J Microbiol 1976; 20: 397-403.