New method for detection of Borrelia burgdorferi antigen complexed to antibody in seronegative Lyme disease

Journal of Immunological Methods

Volumn 249, Issue 1-2, 2001, Pages 185-190

  Brunner, Michael ^a  

^a CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

Author keywords

Borrelia burgdorferi; Borrelia specific antigens; Immune complexes; Lyme disease; OspA; Serologic tests

Indexed keywords

ANTIBODY; BACTERIAL ANTIGEN; CROSS REACTING ANTIGEN; MONOCLONAL ANTIBODY; OUTER SURFACE PROTEIN A; UNCLASSIFIED DRUG;

ANTIBODY DETECTION; ANTIGEN ANTIBODY COMPLEX; ANTIGEN DETECTION; ANTIGEN SPECIFICITY; ARTICLE; BORRELIA BURGDORFERI; BORRELIA INFECTION; COMPLEX FORMATION; CONTROLLED STUDY; DIAGNOSTIC ERROR; DIAGNOSTIC VALUE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOBLOTTING; LYME DISEASE; PRIORITY JOURNAL; SERODIAGNOSIS; SERUM; TECHNIQUE;

ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BORRELIA BURGDORFERI GROUP; HUMANS; LYME DISEASE; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS;

BORRELIA; BORRELIA BURGDORFERI;

EID: 0035283036     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00356-2     Document Type: Article

References (43)

1. The immunoglobulin (IgG) antibody response to OspA and OspB correlates with severe and prolonged Lyme arthritis and the IgG response to P35 correlates with mild and brief arthritis
2. Interlaboratory comparison of test results in the Wisconsin State Laboratory of Hygiene/College of American Pathologists proficiency testing program
3. Performance of 45 laboratories participating in a proficiency testing program for Lyme disease serology
4. Biology of Borrelia species
5. Immune complexes from serum of patients with Lyme disease contain Borrelia burgdorferi antigen and antigen-specific antibodies: Potential use for improved testing
6. Immunoglobulin M capture assay for serologic confirmation of early Lyme disease: Analysis of immune complexes with biotinylated Borrelia burgdorferi sonicate enhanced with Flagellin peptide epitope
7. Enzyme-linked, IgM capture, immune complex, biotinylated antigen assay (EMIBA): A new serologic assay for early Lyme disease
8. Detection of hepatitis B antigen in circulating immune complexes in acute and chronic hepatitis
9. Case definitions for infectious conditions under public health surveillance
10. Recommendations for test performance and interpretation from the Second National Conference on Serological Diagnosis of Lyme disease
11. Case definitions for public health surveillance
12. Detection of Borrelia burgdorferi-specific antigen in antibody-negative cerebrospinal fluid in neurologic Lyme disease
13. Cerebrospinal fluid immune complexes in patients exposed to Borrelia burgdorferi: Detection of Borrelia-specific and -nonspecific complexes
14. Detection and isolation of immune complexes in multiple sclerosis cerebrospinal fluid
15. Detection of circulating immune complexes in human sera by simplified assays with polyethylene glycol
16. Immune capture and detection of Borrelia burgdorferi antigens in urine, blood, tissue from infected ticks, mice, dogs, and humans
17. Western blotting in the serodiagnosis of Lyme disease
18. Protection of mice against the Lyme disease agent by immunizing with recombinant OspA
19. Serological follow-up after treatment of patients with erythema migrans and neuroborreliosis
20. Serologic tests for antibody to Borrelia burgdorferi: Another Pandora's box for medicine?
21. An interlaboratory study of antibody to Borrelia burgdorferi
22. Detection of antibodies after immune complex splitting in serum of patients with bullous pemphigoid and systemic lupus erythematosus
23. Early and late antibody responses to full-length and truncated constructs of outer surface protein A of Borrelia burgdorferi in Lyme disease
24. Purification and immunologic characterization of a major low molecular weight lipoprotein from Borreia burgdorferi
25. Cellular immune reactivity to recombinant OspA and flagellin from Borrelia burgdorferi in patients with Lyme boreliosis. Complexity of humoral and cellular immune responses
26. Interlaboratory and intralaboratory comparison of indirect immunofluorescence assays for serodiagnosis of Lyme disease
27. Serologic tests for Lyme disease: Interlaboratory variability
28. Antigenic conservation of an immunodominant invariable region of the VIsE lipoprotein among European pathogenic genospecies of Borrelia burgdorferi SL
29. Immunoserologic evidence of coinfection with Borrelia burgdorferi, Babesia microti, and human granulocytic Ehrlichia species in residents of Wisconsin and Minnesota
30. Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: Exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease
31. Borrelia burgdorferi-specific immune complexes in acute Lyme disease
32. Simultaneous expression of Borrelia OspA and OspC and IgM response in cerebrospinal fluid in early neurologic Lyme disease
33. Early and specific antibody response to Osp A in Lyme disease
34. Identification of specific Borrelia burgdorferi components in circulating antigen-antibody complexes
35. Sequestration of antibody to Borrelia burgdorferi in immune complexes in seronegative Lyme disease
36. Induction of an outer surface protein on Borrelia burgdorferi during tick feeding
37. Changes in antigenic reactivity of Borrelia burgdorferi, the Lyme disease spirochete, during persistent infection in mice
38. A vaccine consisting of recombinant Borrelia burgdorferi outer-surface protein A to prevent Lyme disease
39. Borrelia burgdorferi OspA is an artropod-specific transmission-blocking Lyme disease vaccine
40. Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant
41. The overdiagnosis of Lyme disease
42. The spirochetal etiology of Lyme disease
43. Detection and preliminary characterization of circulating immune complexes in patients with Lyme disease