Expression purification, and characterization of active recombinant prostate-specific antigen in Pichia pastoris (yeast)

Prostate

Volumn 46, Issue 4, 2001, Pages 298-306

  Habeck, Lillian L ^a   Belagaje, Rama M ^a   Becker, Gerald W ^a   Hale, John E ^a   Churgay, Lisa M ^a   Ulmer, Maverick ^a   Yang, Xiao Yan ^a   Shackelford, Katherine A ^a   Richardson, John M ^a   Johnson, Melvin G ^a   Mendelsohn, Laurane G ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

Prostate cancer; Prostate specific antigen; PSA; Serine protease

Indexed keywords

GLUCAN SYNTHASE; PROSTATE SPECIFIC ANTIGEN; RECOMBINANT PROTEIN;

ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; DNA INTEGRATION; IMMUNOBLOTTING; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN GLYCOSYLATION; PROTEIN SECRETION; RECOMBINANT DNA TECHNOLOGY; SEQUENCE ANALYSIS;

AMIDOHYDROLASES; BLOTTING, WESTERN; DNA PRIMERS; DNA, NEOPLASM; DNA, RECOMBINANT; GENE EXPRESSION REGULATION, FUNGAL; GENE EXPRESSION REGULATION, NEOPLASTIC; GENETIC VECTORS; HUMANS; MALE; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; PICHIA; POLYMERASE CHAIN REACTION; PROSTATE-SPECIFIC ANTIGEN; PROSTATIC NEOPLASMS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 0035282919     PISSN: 02704137     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0045(20010301)46:4<298::AID-PROS1036>3.0.CO;2-J     Document Type: Article

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