Mutations in DnaA protein suppress the growth arrest of acidic phospholipid-deficient Escherichia coli cells

EMBO Journal

Volumn 20, Issue 5, 2001, Pages 1164-1172

  Zheng, Weidong ^a   Li, Zhenya ^a,b   Skarstad, Kirsten ^c   Crooke, Elliott ^a  

^a GEORGETOWN UNIVERSITY MEDICAL CENTER   (United States)

^b CELERA GENOMICS   (United States)

^c OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

DnaA; Escherichia coli; Flow cytometry; Phospholipid; Replication

Indexed keywords

CARBOXYL GROUP; DEOXYRIBONUCLEOPROTEIN; DNA A; PHOSPHOLIPID;

AMINO ACID SUBSTITUTION; ARTICLE; DNA BINDING; DNA REPLICATION; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE DELETION; GENE MUTATION; GROWTH INHIBITION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROCESSING;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CELL DIVISION; CEPHALEXIN; CHROMOSOMES, BACTERIAL; DNA REPLICATION; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; FLOW CYTOMETRY; HYDROGEN-ION CONCENTRATION; ORIGIN RECOGNITION COMPLEX; PHOSPHOLIPIDS; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; RIFAMPIN; SEQUENCE DELETION; SUPPRESSION, GENETIC; TRANSFORMATION, BACTERIAL; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035282783     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.5.1164     Document Type: Article

References (34)

1. Three distinct chromosome replication states are induced by increasing concentrations of DnaA protein in Escherichia coli
2. Bacterial growth control studied by flow cytometry
3. The chromosome origin of E.coli stabilizes DnaA protein during rejuvenation by phospholipids
4. Replicatively active complexes of DnaA protein and the Escherichia coli chromosomal origin observed in the electron microscope
5. The DnaA protein complex with the E.coli chromosomal replication origin (oriC) and other DNA sites
6. In vitro assembly of a prepriming complex at the origin of the Escherichia coli chromosome
7. Membrane regulation of the chromosomal replication activity of E.coli DnaA requires a discrete site on the protein
8. Membrane-mediated release of nucleotide from an initiator of chromosomal replication, E.coli DnaA, occurs with insertion of a distinct region of the protein into the lipid bilayer
9. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
10. Site-directed mutational analysis for the membrane binding of DnaA protein: Identification of amino acids involved in the functional interaction between DnaA protein and acidic phospholipids
11. Alteration of the phospholipid composition of Esherichia coli through genetic manipulation
12. The β-subunit sliding clamp of DNA polymerase III, the E.coli replicase, renders DnaA protein inactive for initiating chromosomal replication
13. Viability of an Escherichia coli pgsA null mutant lacking detectable phosphatidylglycerol and cardiolipin
14. Rapid and efficient site-specific mutagenesis without phenotypic selection
15. Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli
16. Functional analysis of affinity-purified polyhistidine-tagged DnaA protein
17. Identification of amino acids involved in the functional interaction between DnaA protein and acidic phospholipids
18. Separate roles of Escherichia coli replication proteins in synthesis and partitioning of pSC101 plasmid DNA
19. DnaA, the initiator of Escherichia coli chromosomal replication, is located at the cell membrane
20. RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro
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27. Novel alleles of the Escherichia coli dnaA gene
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29. The Escherichia coli SeqA protein destabilizes mutant DnaA204 protein
30. dnaA suppressor (dasF) mutants of Escherichia coli are stable DNA replication (sdrA/rnh) mutants
31. Mode of initiation of constitutive stable DNA replication in RNase H-defective mutants of Escherichia coli K-12
32. The N-terminus promotes oligomerization of the Escherichia coli initiator protein DnaA
33. The initiation mass for DNA replication in Escherichia coli K-12 is dependent on growth rate
34. In vivo evidence for the involvement of anionic phospholipids in initiation of DNA replication in Escherichia coli