Functional analysis of affinity-purified polyhistidine-tagged DnaA protein

Protein Expression and Purification

Volumn 17, Issue 1, 1999, Pages 41-48

  Li, Zhenya ^a   Crooke, Elliott ^a  

^a GEORGETOWN UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

DNA replication; DnaA protein; oriC; Polyhistidine tag; Purification

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; CELL CULTURE; CLONED GENE; DNA ISOLATION; DNA REPLICATION; ESCHERICHIA COLI; EXPRESSED SEQUENCE TAG; GENE EXPRESSION SYSTEM; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTAGENESIS; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN DNA BINDING; PROTEIN LIPID INTERACTION; PROTEIN PURIFICATION; STRUCTURE ACTIVITY RELATION; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0033213098     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1094     Document Type: Article

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