Inositol phosphates from barley low-phytate grain mutants analysed by metal-dye detection HPLC and NMR

Biochemical Journal

Volumn 354, Issue 2, 2001, Pages 473-480

  Hatzack, F ^a   Hubel F ^a   Zhang, W ^a   Hansen, P E ^a   Rasmussen, S K ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Grain filling; Hordeum vulgare; Inositol phosphate kinase; Phytase; Seed specific

Indexed keywords

INOSITOL PHOSPHATE; PHYTATE;

ARTICLE; BARLEY; CHEMICAL ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PHOSPHOINOSITIDE METABOLISM; PLANT SEED; PRIORITY JOURNAL;

6-PHYTASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLORING AGENTS; ETHER, ETHYL; HORDEUM; INOSITOL PHOSPHATES; ISOMERISM; MAGNETIC RESONANCE SPECTROSCOPY; METALS; PHYTIC ACID; TRICHLOROACETIC ACID;

HORDEUM; HORDEUM VULGARE SUBSP. VULGARE;

EID: 0035282067     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3540473     Document Type: Article

References (40)

1. Structure and occurrence of inositols in plants
2. Biochemistry and genetics of phytic acid synthesis
3. Genetics of myo-inositol phosphate synthesis and accumulation
4. Accumulation of seed reserves of phosphorus and other minerals
5. Distribution of phytate and nutritionally important elements among the morphological components of cereal grains
6. Metabolic evidence for order of addition of individual phosphate esters to myo-inositol moiety of inositol hexakisphosphate in the duckweed Spirodela polyrhiza L
7. Characterization of a cDNA encoding Arabidopsis thaliana inositol 1,3,4-trisphosphate 5/6-kinase
8. Identification of inositol 1,3,4-trisphosphate 5-kinase and inositol 1,3,4,5-tetrakisphosphate 6-kinase in immature soybean seeds
9. Purification and some properties of inositol 1,3,4,5,6-pentakisphosphate 2-kinase from immature soybean seeds
10. 3 and osmotic regulation
11. 6 in Dictyostelium
12. The structure of myo-inositol pentaphosphates
13. The phytases I. Lysolecithin activated phytase from wheat bran
14. 2
15. 6 in vitro by wheat-bran phytase
16. Preparation and separation of inositol tetrakisphosphates and inositol pentakisphosphates and the establishment of enantiomeric configurations by the use of L-iditol dehydrogenase
17. Myo-inositol pentakisphosphates. Structure, biological occurrence and phosphorylation to myo-inositol hexakisphosphate
18. Gradient ion chromatography of inositol phosphates
19. Maize root phytase. Purification, characterization, and localization of enzyme activity and its putative substrate
20. Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen
21. Linkage mapping of two mutations that reduce phytic acid content of barley-grain
22. Identification of two low-phytate barley (Hordeum vulgare L.) grain mutants by TLC and genetic analysis
23. Linkage mapping of maize and barley myo-inositol 1-phosphate synthase DNA sequences: Correspondence with a low phytic acid mutation
24. Mass changes in inositol tetrakis phosphate and pentakisphosphate isomers induced by chemotactic peptide stimulation in HI-60 cells
25. Mass determination of inositol phosphates by high-performance liquid chromatography with postcolumn complexometry metal-dye detection
26. Non-radioactive, isomer-specific inositol phosphate mass determinations: High-performance liquid chromatography-micro-metal-dye detection strongly improves speed and sensitivity of analyses from cells and micro-enzyme assays
27. The quantitative spectrum of inositol phosphate metabolites in avian erythrocytes, analysed by proton n.m.r. and h.p.l.c. with direct isomer detection
28. High-performance thin-layer chromatography method for inositol phosphate analysis
29. A novel metal-dye detection system permits picomolar-range h.p.l.c. analysis of inositol polyphosphates from non-radioactively labelled cell or tissue specimens
30. New techniques in the study of biomolecular structure by high field NMR
31. Clean TOCSY for proton spin system identification in macromolecules
32. Nuclear magnetic resonance spectroscopic analysis of myo-inositol phosphates including inositol 1,3,4,5-tetrakisphosphate
33. Confirmation of the identities of inositol 1,3,4-trisphosphate and inositol 1,3,4,5-tetrakisphosphate by the use of one-dimensional and two-dimensional n.m.r. spectroscopy
34. NMR spectroscopy of myo-inositol phosphates
35. Nutritionally relevant parameters in low-phytate barley mutants
36. 2+ from crude microsomes and enriched vesicular plasma membranes, but not from intracellular stores of permeabilized T-lymphocytes and monocytes
37. 2+ release from microsomal fraction and liposomes
38. An inositol 1,4,5-trisphosphate-6-kinase activity in pea roots
39. Origin and seed phenotype of maize low phytic acid 1-1 and low phytic acid 2-1