Improvement of the pBRINT-Ts plasmid family to obtain marker-free chromosomal insertion of cloned DNA in E. coli

BioTechniques

Volumn 30, Issue 2, 2001, Pages 252-256

  Le Borgne, S ^a   Palmeros, B ^a   Bolivar F ^a   Gosset, G ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID VECTOR; RECOMBINASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIOPHAGE P1; CHROMOSOME INSERTION; ESCHERICHIA COLI; GENE CASSETTE; GENE EXPRESSION; HUMAN; LACTOSE OPERON; MARKER GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; RECOMBINANT GENE; REPLICON; TEMPERATURE DEPENDENCE; GENETICS; MOLECULAR GENETICS; PLASMID; TRANSPOSON;

ESCHERICHIA COLI; PROKARYOTA;

BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PLASMIDS; REPLICON;

EID: 0035259881     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01302bm03     Document Type: Article

References (8)

1. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis
2. Construction and characterization of new cloning vehicles, II. A multipurpose cloning system
3. Removal of beta-lactam antibiotics during growth of Escherichia coli strains hosting pUR and pEX vectors
4. s: A plasmid family with a temperature-sensitive replicon, designed for chromosomal integration into the lacZ gene of Escherichia coli
5. Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction
6. A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria
7. A method for construction of E. coli strains with multiple DNA insertions in the chromosome
8. Site-specific recombination: Developments and applications