Sulfated glycans and elevated temperature stimulate PrPsc-dependent cell-free formation of protease-resistant prion protein

EMBO Journal

Volumn 20, Issue 3, 2001, Pages 377-386

  Wong, Cai'ne ^a   Xiong, Liang Wen ^a   Horiuchi, Motohiro ^b   Raymond, Lynne ^a   Wehrly, Kathy ^a   Chesebro, Bruce ^a   Caughey, Byron ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

Author keywords

Heparan sulfate; Pentosan polysulfate; Prion; Scrapie; Transmissible spongiform encephalopathy

Indexed keywords

DETERGENT; GLYCAN; GLYCOSAMINOGLYCAN POLYSULFATE; HEPARAN SULFATE; PENTOSAN POLYSULFATE; PRION PROTEIN; PROTEINASE;

ANIMAL TISSUE; ARTICLE; CELL FREE SYSTEM; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; HAMSTER; HIGH TEMPERATURE; NONHUMAN; PRION; PRION DISEASE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SCRAPIE; SULFATION;

ANIMALS; CELL-FREE SYSTEM; CIRCULAR DICHROISM; CRICETINAE; ENDOPEPTIDASES; HEAT; MICE; PENTOSAN SULFURIC POLYESTER; PRION DISEASES; PROTEIN CONFORMATION; PRPC PROTEINS; PRPSC PROTEINS;

ANIMALIA; CRICETINAE;

EID: 0035253848     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.3.377     Document Type: Article

References (56)

1. Prion rods contain an inert polysaccharide scaffold
2. Opposite effects of dextran sulfate 500, the polyene antibiotic MS-8209 and Congo red on accumulation of the protease-resistant isoform of PrP in the spleens of mice inoculated intraperitoneally with the scrapie agent
3. Nongenetic propagation of strain-specific phenotypes of scrapie prion protein
4. Scrapie susceptibility-linked polymorphisms modulate the in vitro conversion of sheep prion protein to protease-resistant forms
5. Characterization and polyanion-binding properties of purified recombinant prion protein
6. Scrapie associated PrP accumulation and its prevention: Insights from cell culture
7. Scrapie-associated PrP accumulation and agent replication: Effects of sulphated glycosaminoglycan analogues
8. The scrapie-associated form of PrP is made from a cell surface precursor that is both protease- and phospholipase-sensitive
9. Sulfated polyanion inhibition of scrapie-associated PrP accumulation in cultured cells
10. N-terminal truncation of the scrapie-associated form of PrP by lysosomal protease(s): Implications regarding the site of conversion of PrP to the protease-resistant state
11. Secondary structure analysis of the scrapie-associated protein PrP 27-30 in water by infrared spectroscopy
12. Binding of the protease-sensitive form of PrP (prion protein) to sulfated glycosaminoglycan and Congo red
13. Scrapie infectivity correlates with converting activity, protease resistance and aggregation of scrapie-associated prion protein in guanidine denaturation studies
14. Assays of protease-resistant prion protein and its formation
15. Prion diseases - BSE and prions: Uncertainties about the agent
16. Chaperone-supervised conversion of prion protein to its protease-resistant form
17. Structure of the recombinant full-length hamster prion protein PrP(29-231): The N terminus is highly flexible
18. Dextran sulphate 500 delays and prevents mouse scrapie by impairment of agent replication in spleen
19. Prolongation of scrapie incubation period by an injection of dextran sulphate 500 within the month before or after infection
20. Heparin-like molecules bind differentially to prion proteins and change their intracellular metabolic fate
21. Self-replication and scrapie
22. Species-barrier-independent prion replication in apparently resistant species
23. Specific binding of normal prion protein to the scrapie form via a localized domain initiates its conversion to the protease-resistant state
24. Interactions between heterologous forms of prion protein: Binding, inhibition of conversion and species barriers
25. Solution structure of a 142-residue recombinant prion protein corresponding to the infectious fragment of the scrapie isoform
26. Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins
27. Evidence that the transmission of one source of scrapie agent to hamsters involves separation of agent strains from a mixture
28. Suppression of scrapie infection in mice by heteropolyanion 23, dextran sulfate and some other polyanions
29. Temporary and permanent modifications to a single strain of mouse scrapie on transmission to rats and hamsters
30. The genomic identity of different strains of mouse scrapie is expressed in hamsters and preserved on reisolation in mice
31. Cell-free formation of protease-resistant prion protein
32. Species specificity in the cell-free conversion of prion protein to protease-resistant forms: A model for the scrapie species barrier
33. Cell-surface engineering with GPI-anchored proteins
34. Conversion of α-helices into β-sheets features in the formation of the scrapie prion protein
35. A single hamster amino acid blocks conversion to protease-resistant PrP in scrapie-infected mouse neuroblastoma cells
36. Heterologous PrP molecules interfere with accumulation of protease-resistant PrP in scrapie-infected murine neuroblastoma cells
37. Novel proteinaceous infectious particles cause scrapie
38. Purification and structural studies of a major scrapie prion protein
39. Transgenetic studies implicate interactions between homologous PrP isoforms in scrapie prion replication
40. Scrapie infectivity found in resistant species
41. Molecular assessment of the transmissibilities of BSE and scrapie to humans
42. Evidence of a molecular barrier limiting susceptibility of humans, cattle and sheep to chronic wasting disease
43. NMR structure of the mouse prion protein domain PrP(121-231)
44. NMR characterization of the full-length recombinant murine prion protein, mPrP(23-21)
45. Role and specificity of T-cell subsets in spontaneous recovery from Friend virus-induced leukemia in mice
46. Glycosylation differences between the normal and pathogenic prion protein isoforms
47. Eight prion strains have PrP(Sc) molecules with different conformations
48. Sulfated glycans stimulate endocytosis of the cellular isoform of the prion protein, PrPC, in cultured cells
49. Proteoglycans in the pathogenesis of Alzheimer's disease and other amyloidoses
50. Sulfated glycosaminoglycans in amyloid plaques of prion diseases
51. Immunolocalization of heparan sulfate proteoglycans to the prion protein amyloid plaques of Gerstmann-Straussler syndrome, Creutzfeldt-Jakob disease and scrapie
52. Scrapie prion protein contains a phosphatidylinositol glycolipid
53. pH-dependent stability and conformation of the recombinant human prion protein PrP(90-231)
54. Transmission of Creutzfeldt-Jakob disease from humans to transgenic mice expressing chimeric human-mouse prion protein
55. Prion propagation in mice expressing human and chimeric PrP transgenes implicates the interaction of cellular PrP with another protein
56. Determination of protein secondary structure