Role of calcium ions in the structure and function of the di-isopropylfluorophosphatase from Loligo vulgaris

Biochemical Journal

Volumn 353, Issue 3, 2001, Pages 579-589

  Hartleib, J ^a   Geschwindner, S ^a   Scharff, E I ^a   Ruterjans H ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Ca2+ affinity; Ca2+ binding protein; Irreversible inactivation; Organophosphates

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; CALCIUM ION; CHELATING AGENT; DIISOPROPYLFLUOROPHOSPHATASE; PHOSPHATASE; POTASSIUM CHLORIDE; QUIN 2; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CALCIUM BINDING; CATALYSIS; CONFORMATIONAL TRANSITION; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME STRUCTURE; IONIC STRENGTH; LIGHT SCATTERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SQUID; TEMPERATURE DEPENDENCE;

ANIMALS; CALCIUM; CIRCULAR DICHROISM; DECAPODIFORMES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESTERASES; KINETICS; PHOSPHORIC TRIESTER HYDROLASES; PROTEIN BINDING; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; TERBIUM;

ANIMALIA; CEPHALOPODA; LOLIGO VULGARIS;

EID: 0035253077     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3530579     Document Type: Article

References (55)

1. Diisopropylphosphorofluoridate and tabun: Enzymatic hydrolysis and nerve function
2. Characterization of a DFP-hydrolyzing enzyme in squid posterior salivary gland by use of soman, DFP and manganous ion
3. Comparison of DFP-hydrolyzing enzyme purified from head ganglion and hepatopancreas of squid (Loligo pealei) by means of isoelectric focusing
4. Comparison of organophosphorus acid anhydrolases from different species using monoclonal antibodies
5. High yield expression, purification and characterization of the recombinant diisopropylfluorophosphatase from Loligo vulgaris
6. Systematics in the interaction of metal ions with the mainchain carbonyl group in protein structures
7. Interaction of metal ions with carboxylic and carboxamide groups in protein structures
8. Structural aspects of metal liganding to functional groups in proteins
9. Calcium-binding sites in proteins: A structural perspective
10. 2+ to bovine αlactalbumin in the absence and presence of EGTA
11. Role of bound calcium ions in thermostable, proteolytic enzymes. Separation of intrinsic and calcium ion contributions to the kinetic thermal stability
12. Three dimensional structure of porcine pancreatic alpha-amylase at a 2.9 Å resolution. Role of calcium in structure and activity
13. A novel calcium binding site in the galactose-binding protein of bacterial transport and chemotaxis
14. 2
15. 2
16. Fragmentation of bovine serum albumin by pepsin. I. The origin of the acid expansion of the albumin molecule
17. 9k
18. 2+-protein complexes
19. Characterization of the sources of protein-ligand affinity: 1-sulfonato-8-(1′)anilinonaphtalene binding to intestinal fatty acid binding protein
20. 9k
21. The kinetics of calcium binding to calmodulin: Quin 2 and ANS stopped-flow fluorescence studies
22. Organophosphorus acid (OPA) anhydrase from squid: A calcium-dependent P-F-splitting enzyme
23. Evidence for the interaction between the calcium indicator 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid and calcium-binding proteins
24. Energetics of folding subtilisin BPN′
25. Lanthanide ion luminescence probes of the structure of biological macromolecules
26. Terbium(III) emission as a probe of calcium(II) binding sites in proteins
27. 3+ binding to bovine prothrombin and bovine prothrombin fragment 1
28. Terbium probe of calcium-binding sites on the prothrombin-membrane complex
29. Evidence for the non-filamentous aggregation of actin induced by lanthanide ions
30. 2+ binding to a high affinity site that does not contain γ-carboxyglutamic acid
31. The TyrR protein of Escherichia coli analysis by limited proteolysis of domain structure and ligand-mediated conformational changes
32. Probing the domain structure and ligand-induced conformational changes by limited proteolysis of tyrocidine synthetase 1
33. Study of the 'molten globule' intermediate state in protein folding by a hydrophobic fluorescent probe
34. Bovine myeloperoxidase and lactoperoxidase each contain a high affinity site for calcium
35. Novel type of very high affinity calcium-binding sites in β-hydroxy-asparagine containing epidermal growth factor-like domains in Vitamin K-dependent protein S
36. Calcium binding to calmodulin and its globular domains
37. 9k: Stopped-flow studies
38. 2+ exchange with troponin C
39. Stability of protein structure and hydrophobic interaction
40. Dynamic and structural properties of the calcium binding site of bovine serine proteases and their zymogens. A multinuclear nuclear magnetic resonance and stopped-flow study
41. The high affinity calcium-binding sites in the epidermal growth factor module region of vitamin K-dependent-protein S
42. The role of protein surface charges in ion binding
43. Solution structure of a pair of calcium-binding epidermal growth factor-like domains: Implications for the Marfan syndrome and other genetic disorders
44. 2+ binding and dissociation
45. Model for inactivation of α-amylase in the presence of salts: Theoretical and experimental studies
46. Cation binding to a Bacillus (1,3-1,4)-β-glucanase. Geometry, affinity and effect on protein stability
47. Comparison of purified human and rabbit paraoxonases
48. Human liver paraoxonase (PON1): Subcellular distribution and characterization
49. EDTA-resistant and sensitive phosphotriesterase activities associated with albumin and lipoproteins in rabbit serum
50. Calcium binding by human and rabbit serum paraoxonases. Structural stability and enzymatic activity
51. Pertubations to the active site of phosphotriesterase
52. Tryptophan residue(s) as major components of the human serum paraoxonase active site