Comparison of two-photon excitation laser scanning microscopy with UV-confocal laser scanning microscopy in three-dimensional calcium imaging using the fluorescence indicator Indo-1

Journal of Microscopy

Volumn 185, Issue 1, 1997, Pages 9-20

  Sako, Y ^a   Sekihata, A ^a   Yanagisawa, Y ^a   Yamamoto, M ^b   Shimada, Y ^b   Ozaki, K ^b   Kusumi, A ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b OLYMPUS CORPORATION   (Japan)

Author keywords

Calcium imaging; Confocal microscopy; Indo 1; Photobleaching; Phototoxicity; Scanning microscopy; Spatial resolution; Two photon excitation

Indexed keywords

CALCIUM; CELLS; CYTOLOGY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; IMAGE RESOLUTION; LASER EXCITATION; PHOTOBLEACHING; PHOTONS; SIGNAL TO NOISE RATIO; SURFACE ANALYSIS;

CALCIUM CONCENTRATION; CALCIUM IMAGING; CONFOCAL LASER SCANNING; INDO-1; LASER SCANNING FLUORESCENCE MICROSCOPY; PHOTO-TOXICITY; SCANNING MICROSCOPY; SPATIAL RESOLUTION; TWO-PHOTON EXCITATIONS; UV EXCITATION;

CONFOCAL MICROSCOPY;

CALCIUM;

ARTICLE; FLUORESCENCE; IMAGING; LASER MICROSCOPY; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY;

EID: 0031027305     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1997.1480707.x     Document Type: Article

References (14)

1. Art, J. & Goodman, M.B. (1993) Rapid scanning confocal microscopy. Methods Cell Biol. 38, 47-78.
2. Deitche, J., Kempe, M. & Rudolph, W. (1994) Resolution in nonlinear laser scanning microscopy. J. Microsc. 174, 69-73.
3. Denk, W. (1994) Two-photon scanning photochemical microscopy: mapping ligand-gated ion channel distributions. Proc. Natl. Acad. Sci. USA, 91, 6629-6633.
4. Denk, W., Strickler, J.H. & Webb, W.W. (1990) Two-photon laser scanning fluorescence microscopy. Science, 248, 73-76.
5. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3459.
6. 2+ measured by confocal laser-scanning microscopy in bullfrog sympathetic ganglion cells. Neurosci. Res. 10, 245-259.
7. Majlof, L. & Forsgren, P.O. (1993) Confocal microscopy: important considerations for accurate imaging. Methods Cell Biol. 18, 79-95.
8. Nakamura, O. (1993) Three-dimensional imaging characteristics of laser scan fluorescence microscopy: two-photon excitation vs. single-photon excitation. Optik, 93, 39-42.
9. Piston, D. W., Kirby, M.S., Cheng, H., Lederer, W.J. & Webb, W.W. (1994) Two-photon-excitation fluorescence imaging of three-dimensional calcium-ion activity. Appl. Opt. 33, 662-669.
10. Stelzer, E.H.K., Hell, S., Lindek, S., Stricker, R., Pick, R., Storz, C., Ritter, G. & Salmon, N. (1994) Nonlinear absorption extends confocal fluorescence microscopy into the ultra-violet regime and confines the illumination volume. Opt. Commun. 104, 223-228.
11. Ulfhake, B., Carlsson, K., Mossberg, K., Arvidsson, U. & Helm, P.J. (1991) Imaging of fluorescent neurons labeled with Fluoro Gold and fluorescent axon terminals labeled with AMCA (7-amino-4-methylcoumarine-3-acetic acid) conjugated antiserum using a UV-laser confocal scanning microscope. J. Neurosci. Methods, 40, 39-48.
12. Wells, K.S., Sandison, D.R., Strickler, J. & Webb, W.W. (1990) Quantitative fluorescence imaging with laser scanning confocal microscopy. Handbook of Biological Confocal Microscopy (ed. by J. B. Pawly), pp. 27-39. Plenum Press, New York.
13. White, J.G., Amos, W.B. & Fordham, M. (1987) An evaluation of confocal vs. conventional imaging of biological structures by florescence light microscopy. J. Cell Biol. 105, 41-48.
14. Wilson, T. & Sheppard, C. (1984) Theory and Practice of Scanning Optical Microscopy. Academic Press, London.