Methods for identification of centrosome-associated proteins

Methods in Cell Biology

Volumn 67, Issue 67, 2001, Pages 125-140

  Kuriyama, Ryoko ^a   Ohta, Toshiro ^a   Vogel, Jacalyn ^b   Peng, Gang ^b  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0035232040     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (23)

1. Ausubel, F. M., Bent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (1994). "Current Protocols in Molecular Biology." Wiley, New York.
2. Blackburn, G. R., Barreau, M. D., and Dewey, W. C. (1978). Partial purification of centrosomes from Chinese hamster ovary cells. Exp. Cell Res. 113, 183-187.
3. Bornens, M., and Moudjou, M. (1999). Studying the composition and function of centrosomes in vertebrates. Methods Cell Biol. 61, 13-34.
4. Gosti-Testu, F., Marthy, M. C., Berges, J., Maunoury, R., and Bornens, M. (1986). Identification of centrosomal proteins in human lymphoblastic cell line. EMBO J. 5, 2245-2250.
5. Kalt, A., and Schiwa, M. (1993). Molecular components of the centrosome. Trends Cell Biol. 3, 118-128.
6. Kimble, M., and Kuriyama, R. (1992). Functional components of microtubule-organizing centers. Int. Rev. Cytol. 136, 1-50.
7. Komesli, S., Toumier, F., Paintrand, M., Margolis, R. L., Job, D., and Bornens, M. (1989). Mass isolation of calf thymus centrosomes: Identification of a specific configuration. J. Cell Biol. 109, 2869-2878.
8. Kuriyama, R. (1986). Isolation of sea urchin spindles and cytasters. Methods Enzymol. 134, 190-199.
9. Kuriyama, R. (1991). Monoclonal antibodis to MTOC-antigens. In "Centrosomes" (V. Kalnins, ed.), pp. 131-165. Academic Press, San Diego.
10. Kuriyama, R., and Ensrud, K. (1999). Obtaining antibodies to spindle components. Methods Cell Biol. 61, 233-244.
11. Kuriyama, R., Keryer, G., and Borisy, G. G. (1984). The mitotic spindle of Chinese hamster ovary cells isolated in taxol-containing medium. J. Cell Sci. 66, 265-275.
12. Matuliene, J., Essner, R., Ryu, J.-H., Hamaguchi, Y., Baas, P. W., Haraguchi, T., Hiraoka, Y., and Kuriyama, R. (1999). Function of a minus-end-directed kinesin-like motor protein in mammalian cells. J. Cell Sci. 112, 4041-4050.
13. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982). "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
14. Mitchison, T. J., and Kirschner, M. W. (1984). Microtubule assembly nucleated by isolated centrosomes. Nature 312, 232-237.
15. Moritz, M., and Alberts, B. M. (1999). Isolation of centrosomes from Drosophila embryos. Methods Cell Biol. 61, 1-12.
16. Moritz, M., Braunfield, M. B., Fung, J. C., Sedat, J. W., Alberts, B. M., and Agard, D. A. (1995). Three-dimensional structural characterization of centrosomes from early Drosophila embryos. J. Cell Biol. 130, 1149-1159.
17. Ohta, T., Vogel, J., Peng, G., Palazzo, R., and Kuriyama, R. (1994). Analysis of centrosomal components with monoclonal antibodies. Mol. Biol. Cell 5, 37a.
18. Palazzo, R. E., and Vogel, J. M. (1999). Isolation of centrosomes from Spisula solidissima oocytes. Methods Cell Biol. 61, 35-56.
19. Sellitto, C., Kimble, M., and Kuriyama, R. (1992). Heterogeneity of MTOC-components as revealed by monoclonal antibodies to mammalian centrosomes and to nucleus associated bodies from Dictyostelium. Cell Motil. Cytoskel. 22, 7-24.
20. Smith, D. B., and Johnson, K. S. (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
21. Stearns, T., and Winey, M. (1997). The cell center at 100. Cell 91, 303-309.
22. Vogel, J. M., Stearns, T., Rieder, C. L., and Palazzo, R. E. (1997). Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of alpha/beta tubulin. J. Cell Biol. 137, 193-202.
23. Wigge, P. A., Jensen, O. N., Holmes, S., Soues, S., Mann, M., and Kilmartin, J. V. (1998). Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J. Cell Biol. 18, 967-977.