Molecular cloning, characterization, and expression of wheat cystatins

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 1, 2001, Pages 22-28

  Kuroda, Masaharu ^a,b   Kiyosaki, Toshihiro ^b   Matsumoto, Ichiro ^b   Misaka, Takumi ^b   Arai, Soichi ^b   Abe, Keiko ^b  

^a National Agricultural Research Center   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cystatin; Cysteine proteinase; Cysteine proteinase inhibitor; Triticum aestivum; Wheat seed

Indexed keywords

TRITICUM AESTIVUM;

CATHEPSIN; CYSTATIN; CYSTEINE PROTEINASE INHIBITOR; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; CYTOLOGY; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; GERMINATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PLANT SEED; RAT; SEQUENCE ALIGNMENT; SOUTHERN BLOTTING; WHEAT;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, SOUTHERN; CATHEPSINS; CLONING, MOLECULAR; CYSTATINS; CYSTEINE PROTEINASE INHIBITORS; GERMINATION; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; RATS; SEEDS; SEQUENCE ALIGNMENT; TRITICUM;

EID: 0035228375     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.22     Document Type: Article

References (29)

1. Fincher, G. B., Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Ann. Rev. Plant Physiol. Mol. Biol., 40, 305-346 (1989).
2. Koehler, S. and Ho, T. H. D., Hormonal regulation, processing, and secretion of cysteine proteinases in barley aleuron layers. Plant Cell., 2, 769-783 (1990).
3. Koehler, S. and Ho, T. H. D., A major gibberellic acid-induced barley aleuron cysteine proteinase which digests hordein. Plant Physiol., 94, 251-258 (1990).
4. de Barros, E. G. and Larkins, B. A., Cloning of a cDNA encoding a putative cysteine protease from germinating maize seeds. Plant Sci., 99, 189-197 (1994).
5. Domoto, C., Watanabe, H., Abe, M., Abe, K., and Arai, S., Isolation and characterization of two distinct cDNA clones encoding corn seed cysteine proteinase. Biochim. Biophys. Acta, 1263, 241-244 (1995).
6. Watanabe, H., Abe, K., Emori, Y., Hosoyama, H., and Arai, S., Molecular cloning and gibberellin-induced expression of multiple cysteine proteinases of rice seeds (Oryzains). J. Biol. Chem., 266, 16897-16902 (1991).
7. Kato, H. and Minamikawa, T., Identification and characterization of a rice cysteine endopeptidase that digests glutelin. Eur. J. Biochem., 239, 310-316 (1996).
8. Jones, R. L. and Jacobson, J. V., Regulation of synthesis and transport of secreted proteins in cereal aleuron. Int. Rev. Cytol., 126, 49-88 (1991).
9. Barrett, A. J., Rawlings, N. D., Davies, M. E., Machleidt, M. E., Salvensen, G., and Turk, V., Cysteine proteinase inhibitors of the cystatin superfamily. In "Proteinase Inhibitors", Elsevier Science Publishers, Amsterdam (1986).
10. Abe, K., Emori, Y., Kondo, H., Suzuki, K., and Arai, S., Molecular cloning of a cysteine proteinase inhibitor of rice (oryzacystatin). J. Biol. Chem., 262, 16793-16797 (1987).
11. Kondo, H., Abe, K., Nishimura, I., Watanabe, H., and Arai, S., Two distinct cystatin species in rice seeds with different specificities against cysteine proteinases. J. Biol. Chem., 265, 15832-15837 (1990).
12. Abe, M., Abe, K., Kuroda, M., and Arai, S., Corn kernel cysteine proteinase inhibitor as a novel cystatin superfamily member of plant origin. Eur. J. Biochem., 209, 933-937 (1992).
13. Fernandes, K. V. S., Sabelli, P. A., Barratt, D. H. P., Richardson, M., Xavier-Filho, J., and Shewry, P. R., The resistance of cowpea seeds to bruchid beetles is not related to levels of cysteine proteinase inhibitors. Plant Mol. Biol., 23, 215-249 (1993).
14. Abe, M., Abe, K., Domoto, C., and Arai, S., Two distinct species of corn cystatin in corn kernels. Biosci. Biotechnol. Biochem., 59, 756-758 (1995).
15. Botella, M. A., Xu, Y., Prabha, T. N., Zhao, Y., Narasimhan, M. L., Wilson, K. A., Nielsen, S. S., Bressan, R. A., and Hasegawa, P. M., Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate. Plant Physiol., 112, 1201-1210 (1996).
16. Misaka, T., Kuroda, M., Iwabuchi, K., Abe, K., and Arai, S., Soyacystatin, a novel cysteine proteinase inhibitor in soybean, is distinct in protein structure and gene organization from other cystatins of animal and plant origin. Eur. J. Biochem., 240, 609-614 (1997).
17. Abe, K., Kondo, H., Watanabe, H., Emori, Y., and Arai, S., Oryzacystatins as the first well-defined cystatins of plant origin and their target proteinases in rice seeds. Biomed. Biochim. Acta, 50, 637-641 (1991).
18. Chen, M. S., Johnson, B., Wen, L., Muthukrishnan, S., Kramer, K. J., Morgan, T. D., and Reeck, G. R., Rice cystatin: bacterial expression, purification, cysteine proteinase inhibitory activity, and insect growth suppressing activity of a truncated form of the protein. Protein Express. Purif., 3, 41-49 (1992).
19. Orr, G. L., Strickland, J. A., and Walsh, T. A., Inhibition of Diabrotica larval growth by a multicystatin from potato tubers. J. Insect Physiol., 40, 893-900 (1994).
20. Urwin, P. E., Atkinson, H. J., Waller, D. A., and McPherson, M. J., Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida. Plant J., 8, 121-131 (1995).
21. Kuroda, M., Ishimoto, M., Suzuki, K., Kondo, H., Abe, K., Kitamura, K., and Arai, S., Oryzacystatins exhibits growth-inhibitory and lethal effects on different species of bean insect pests, Callosobruchus chinensis (Coleoptera) and Riptortus clavatus (Hemiptera). Biosci. Biotechnol. Biochem., 60, 209-212 (1996).
22. Edmonds, H. S., Gatehouse, L. N., Hilder, V. A., and Gatehouse, J. A., The inhibitory effects of the cysteine protease inhibitor, oryzacystatin, on digestive proteases and on larval survival and development of the southern corn rootworm (Diabrotica undecimpunctata howardi). Entomol. Exp. Appl., 78, 83-94 (1996).
23. Halverson, J. and Zeleny, L., Criteria of wheat quality. In "Wheat: Chemistry and Technology" 3rd ed., American Association of Cereal Chemistry Inc., Minnesota, pp. 15-46 (1988).
24. Kuroda, M., Kiyosaki, T., Arai, S., and Abe, K., Purification and properties of a cysteine proteinase occurring in dormant wheat seeds. Biosci. Biotechnol. Biochem., 61, 732-734 (1997).
25. Shirzadegan, M., Christie, P., and Seemann, J. R., An efficient method for isolation of RNA from tissue cultured plant cells. Nuc. Acids Res., 19, 6055 (1991).
26. 2-terminal 21 amino acid residues are not essential for the papain-inhibitory activity of oryzacystatin, a member of the cystatin superfamily. J. Biol. Chem., 263, 7655-7659 (1988).
27. Barrett, A. J. and Kirschke, H., Cathepsin B, cathepsin H, and cathepsin L. Meth. Enzymol., 80, 535-561 (1981).
28. Isemura, S., Saitoh, H., and Sanada, K., Characterization and amino acid sequence of a new acidic cysteine proteinase inhibitor (cystatin SA) structurally closely related to cystatin S, from human whole saliva. J. Biochem., 102, 693-704 (1987).
29. Dixon, M. and Webb, E. C., Enzyme inhibition and activation. In "The Enzymes" Longmans, London, pp. 332-380 (1979).