Antibodies against the Ki-67 protein: Assessment of the growth fraction and tools for cell cycle analysis

Methods in Cell Biology

Volumn 63, Issue , 2001, Pages 399-418

  Endl, Elmar ^a   Hollmann, Christiane ^a   Gerdes, Johannes ^a  

^a RESEARCH CENTER BORSTEL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; KI 67 ANTIGEN; MONOCLONAL ANTIBODY;

ANIMAL; ARTICLE; CELL CYCLE; CELL LINE; CELL SEPARATION; FLOW CYTOMETRY; HUMAN; IMMUNOLOGY; METABOLISM; METHODOLOGY; MOUSE; RAT; TISSUE FIXATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL CYCLE; CELL LINE; CELL SEPARATION; DNA; FLOW CYTOMETRY; HUMANS; KI-67 ANTIGEN; MICE; RATS; TISSUE FIXATION;

EID: 0035220186     PISSN: 0091679X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0091-679X(01)63022-X     Document Type: Review

References (83)

1. Aubin, J. E. (1979). Autofluorescence of viable cultured mammalian cells. J. Histochem. Cytochem. 27, 36-43.
2. Bading, H., Rauterberg, E. W., and Moelling, K. (1989). Distribution of c-myc, c-myb, and Ki-67 antigens in interphase and mitotic human cells evidenced by immunofluorescence staining technique. Exp. Cell Res. 185, 50-59.
3. Baisch, H., and Gerdes, J. (1987). Simultaneous staining of exponentially growing versus plateau phase cells with the proliferation-associated antibody Ki-67 and propidium iodide: Analysis by flow cytometry. Cell Tissue Kinet. 20, 387-391.
4. Baisch, H., and Gerdes, J. (1990). Identification of proliferating cells by Ki-67 antibody. In "Methods in Cell Biology" (Z. Darzynkiewicz, J. P. Robinson, and H. A. Crissman, eds.), Vol. 41, pp. 217-226. Academic Press, New York.
5. Bauer, K. D., and Jacobberger, J. W. (1994). Analysis of intracellular proteins. In "Methods in Cell Biology" (Z. Darzynkiewicz, J. P. Robinson, and H. A. Crissman, eds.), Vol. 41, pp. 351-376. Academic Press, New York.
6. Benson, H. C., Meyer, R. A., and Zaruba M. E. (1979). Cellular autofluorescence - Is it due to flavins? J. Histochem. Cytochem. 27, 44-48.
7. Braun, N., Papadopoulos, T., and Muller-Hermelink, H. K. (1988). Cell cycle dependent distribution of the proliferation-associated Ki-67 antigen in human embryonic lung cells. Virch. Arch. B Cell Pathol. Ind. Mol. Pathol. 56, 25-33.
8. Bridger, J. M., Kill, I. R., and Lichter, P. (1998). Association of pKi-67 with satellite DNA of the human genome in early G1 cells. Chromosome Res. 6, 13-24.
9. Brown, D. C., and Gatter, K. C. (1990). Monoclonal antibody Ki-67: Its use in histopathology. Histopathology 17, 489-503.
10. Bruno, S., and Darzynkiewicz, Z. (1992). Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells. Cell Prolif. 25, 31-40.
11. Bruno, S., Gorczyca, W., and Darzynkiewicz, Z. (1992). Effect of ionic strength in immunocytochemical detection of the proliferation associated nuclear antigens p120, PCNA, and the protein reacting with Ki-67 antibody. Cytometry 13, 496-501.
12. Campana, D., Coustan-Smith, E., and Janossy, G. (1988). Double and triple staining methods for studying the proliferative activity of human B and T lymphoid cells. J. Immunol. Methods 107, 79-88.
13. Camplejohn, R. S. (1994). The measurement of intracellular antigens and DNA by multiparametric flow cytometry. J. Microsc. 176, 1-7.
14. Cattoretti, G., Becker, M. H., Key, G., Duchrow, M., Schluter, C., Galle, J., and Gerdes, J. (1992). Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J. Pathol. 168, 357-363.
15. Cattoretti, G., Pileri, S., Parravicini, C., Becker, M. H., Poggi, S., Bifulco, C., Key, G., D'Amato, L., Sabattini, E., and Feudale, E. (1993). Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J. Pathol. 171, 83-98.
16. Cavanagh, L. L., Saal, R. J., Grimmett, K. L., and Thomas, R. (1998). Proliferation in monocyte-derived dendritic cell cultures is caused by progenitor cells capable of myeloid differentiation. Blood 92, 1598-1607.
17. Clevenger, C. V., and Shankey, T. V. (1993). Cytochemistry II: Immunofluorescence measurement of intracellular antigens. In "Clinical Flow Cytometry" (K. D. Bauer, R. E. Duque, and T. V. Shankey, eds.), pp. 157-176. Williams & Wilkins, Baltimore.
18. Coates, P. J., Hales, S. A., and Hall, P. A. (1996). The association between cell proliferation and apoptosis: Studies using the cell cycle-associated proteins Ki67 and DNA polymerase a. J. Pathol. 178, 71-77.
19. Duchrow, M., Schluter, C., Wohlenberg, C., Flad, H. D., and Gerdes, J. (1994). Molecular characterization of the gene locus of the human cell proliferation-associated nuclear protein defined by monoclonal antibody Ki-67. Cell Prolif. 29, 1-12.
20. Du Manoir, M. S., Guillaud, P., Camus, E., Seigneurin, D., and Brugal, G. (1991). Ki-67 labeling in postmitotic cells defines different Ki-67 pathways within the 2c compartment. Cytometry 12, 455-463.
21. Endl, E., and Gerdes, J. (2000). Post-translational modifications of the Ki-67 protein coincide with two major check points during mitosis. J. Cell. Physiol. 182, 371-380.
22. Endl, E., Steinbach, P., Knuchel, R., and Hofstadter, F. (1997). Analysis of cell cycle-related Ki-67 and p120 expression by flow cytometric BrdUrd-Hoechst/7AAD and immunolabeling technique. Cytometry 29, 233-241.
23. Gerdes, J. (1985). An immunohistological method for estimating cell growth fractions in rapid histopathological diagnosis during surgery. Int. J. Cancer 35, 169-171.
24. Gerdes, J. (1990). Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin.Cancer Biol. 1, 199-206.
25. Gerdes, J., Schwab, U., Lemke, H., and Stein, H. (1983). Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int. J. Cancer 31, 13-20.
26. Gerdes, J., Lemke, H., Baisch, H., Wacker, H. H., Schwab, U., and Stein, H. (1984a). Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J. Immunol. 133, 1710-1715.
27. Gerdes, J., Dallenbach, F., Lennert, K., Lemke, H., and Stein, H. (1984b). Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol. Oncol. 2, 365-371.
28. Gerdes, J., Pickartz, H., Brotherton, J., Hammerstein, J., Weitzel, H., and Stein, H. (1987a). Growth fractions and estrogen receptors in human breast cancers as determined in situ with monoclonal antibodies. Am. J. Pathol. 129, 486-492.
29. Gerdes, J., Van Baarlen, J., Pileri, S., Schwarting, R., Van Unnik, J. A., and Stein, H. (1987b). Tumor cell growth fraction in Hodgkin's disease. Am. J. Pathol. 128, 390-393.
30. Gerdes, J., Li, L., Schlueter, C., Duchrow, M., Wohlenberg, C., Gerlach, C., Stahmer, I., Kloth, S., Brandt, E., and Flad, H. D. (1991). Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67. Am. J. Pathol. 138, 867-873.
31. Gerdes, J., Becker, M. H., Key, G., and Cattoretti, G. (1992). Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J. Pathol. 168, 85-86.
32. Gerlach, C., Golding, M., Larue, L., Alison, M. R., and Gerdes, J. (1997). Ki-67 immunoexpression is a robust marker of proliferative cells in the rat. Lab. Invest. 77, 697-698.
33. Grogan, T. M., Lippman, S. M., Spier, C. M., Slymen, D. J., Rybski, J. A., Rangel, C. S., Richter, L. C., Miller, T. P. (1988). Independent prognostic significance of a nuclear proliferation antigen in diffuse large cell lymphomas as determined by the monoclonal antibody Ki-67. Blood 71, 1157-1160.
34. Hall, P. A., Richards, M. A., Gregory, W. M., d'Ardenne, A. J., Lister, T. A., and Stansfeld, A. G. (1988). The prognostic value of Ki67 immunostaining in non-Hodgkin's lymphoma. J. Pathol. 154, 223-235.
35. Hall, P. A., McKee, P. H., Menage, H. D., Dover, R., and Lane, D. P. (1993). High levels of p53 protein in UV-irradiated normal human skin. Oncogene 8, 203-207.
36. Heidebrecht, H. J., Buck, F., Haas, K., Wacker, H. H., and Pawaresch R. (1996). Monoclonal antibodies Ki-S3 and Ki-S5 yield new data on the "Ki-67" proteins. Cell Prolif. 29, 413-425.
37. Heslin, M. J., Cordon-Cardo, C., Lewis, J. J., Woodruff, J. M., and Brennan, M. F. (1998). Ki-67 detected by MIB-1 predicts distant metastasis and tumor mortality in primary high grade extremity soft tissue sarcoma. Cancer 83, 490-497.
38. Jacobberger, J. W. (1991). Intracellular antigen staining: Quantitative immunofluorescence. Methods 2, 207-218.
39. Jansen, R. L., Hupperets, P. S., Arends, J. W., Joosten-Achjanie, S. R., Volovics, A., Schouten, H. C., and Hillen, H. F. (1998). MIB-1 labelling index is an independent prognostic marker in primary breast cancer. Br. J. Cancer 78, 460-465.
40. Jares, P., Campo, E., Pinyol, M., Bosch, F., Miquel, R., Fernandez, P. L., Sanchez-Beato, M., Soler, F., Perez-Losada, A., Nayach, I., Mallofre, C., Piris, M. A., Montserrat, E., and Cardesa, A. (1996). Expression of retinoblastorna gene product (pRb) in mantle cell lymphomas. Correlation with cyclin D1 (PRAD1/CCND1) mRNA levels and proliferative activity. Am. J. Pathol. 148, 1591-1600.
41. Key, G., Becker, M. H. G., Baron, B., Duchrow, M., Schlueter, C., Flad, H. D., and Gerdes, J. (1993). New Ki-67 equivilant murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 66bp repetitive elements encoding for the Ki-67 epitope. Lab. Invest. 68, 629-635.
42. Kill, I. R. (1996). Localisation of the Ki-67 antigen within the nucleolus. Evidence for a fibrillarin-deficient region of the dense fibrillar component. J. Cell Sci. 109, 1253-1263.
43. Kute, T. E., and Quadri, Y. (1991). Measurement of proliferation nuclear and membrane markers in tumor cells by flow cytometry. J. Histochem. Cytochem. 39, 1125-1130.
44. Landberg, G., and Roos, G. (1992). Flow cytometric analysis of proliferation associated nuclear antigens using washless staining of unfixed cells. Cytometry 13, 230-240.
45. Landberg, G., Tan, E. M., and Roos, G. (1990). Flow cytometric multiparameter analysis of proliferat-ing cell nuclear antigen/cyclin and Ki-67 antigen: A new view of the cell cycle. Exp. Cell Res. 187, 111-118.
46. Larsen, J. K., Christensen, I. J., Christiansen, J., and Mortensen, B. J. (1991). Washless double staining of unfixed nuclei for flow cytometric analysis of DNA and nuclear antigen (Ki-67 or bromodeoxyuridine). Cytometry 12, 429-437.
47. Littleton, R. J., Baker, G. M., Soomro, I. N., Adams, R. L., and Whimster, W. F. (1991). Kinetic aspects of Ki-67 antigen expression in a normal cell line. Virch. Arch. B Cell Pathol. Ind. Mol. Pathol. 60, 15-19.
48. Lopez, F., Belloc, F., Lacombe, F., Dumain, P., Reiffers, J., Bernard, P., and Boisseau, M. R. (1991). Modalities of synthesis of Ki-67 antigen during the stimulation of lymphocytes. Cytometry 12, 42-49.
49. MacCallum, D. E., and Hall, P. A. (1999). Biochemical characterization of pKi67 with the identification of a mitotic-specific form associated with hyperphosphorylation and altered DNA binding. Exp. Cell Res. 252, 186-198.
50. McCormick, D., Chong, H., Hobbs, C., Datta, C., and Hall, P. A. (1993a). Detection of the Ki-67 antigen in fixed and wax-embedded sections with the monoclonal antibody MIB1. Histopathology 22, 355-360.
51. McCormick, D., Yu, C., Hobbs, C., and Hall, P. A. (1993b). The relevance of antibody concentration to the immunohistological quantification of cell proliferation-associated antigens. Histopathology 22, 543-547.
52. Naiem, M., Gerdes, J., Abdulaziz, Z., Stein, H., and Mason, D. Y. (1983). Production of a monoclonal antibody reactive with human dendritic reticulum cells and analysis of human lymphoid tissue. J. Clin. Pathol. 36, 167-175.
53. Neubauer, A., Serke, S., Siegert, W., Kroll, W., Musch, R., and Huhn, D. (1989). A flow cytometric assay for the determination of cell proliferation with a monoclonal antibody directed against DNA-methyltransferase. Br. J. Haematol. 72, 492-496.
54. Overton, W. (1988). Modified histogram subtraction technique for analysis of flow cytometry data. Cytometry 9, 619-626.
55. Palutke, M., KuKuruga, D., and Tabaczka, P. (1987). A flow cytometric method for measuring lymphocyte proliferation directly from tissue culture plates using Ki-67 and propidium iodide. J. Immunol. Methods 105, 97-105.
56. Pavelic, Z. P., Pavelic, L., Lower, E. E., Gapany, M., Gapany, S., Barker, E. A., and Preisler, H. D. (1992). c-myc, c-erbB-2, and Ki-67 expression in normal breast tissue and in invasive and noninvasive breast carcinoma. Cancer Res. 52, 2597-2602.
57. Pellicciari, C, Mangiarotti, R., Bottone, M. G., Danova, M., and Wang, E. (1995). Identification of resting cells by dual-parameter flow cytometry of statin expression and DNA content. Cytometry 21, 329-337.
58. Perry, A., Stafford, S. L., Scheithauer, B. W., Suman, V. J., and Lohse, C. M. (1998). The prognostic significance of MIB-1, p53, and DNA flow cytometry in completely resected primary meningiomas. Cancer 82, 2262-2269.
59. Pittman, S. M., Strickland, D., and Ireland, C. M. (1994). Polymerization of tubulin in apoptotic cells is not cell cycle dependent. Exp. Cell Res. 215, 263-272.
60. Popov, Z., Hoznek, A., Colombel, M., Bastuji-Garin, S., Lefrere-Belda, M. A., Bellot, J., Abboh, C. C., Mazerolles, C., and Chopin, D. K. (1997). The prognostic value of p53 nuclear overexpression and MIB-1 as a proliferative marker in transitional cell carcinoma of the bladder. Cancer 80, 1472-1481.
61. Rechsteiner, M., and Rogers, S. W. (1996). PEST sequences and regulation by proteolysis. Trends Biochem. Sci. 21, 267-271.
62. Ruby, S. G., and McNally, A. C. (1996). Quality control of proliferation marker (MIB-1) in image analysis systems utilizing cell culture based control materials. Am. J. Clin. Pathol. 106, 634-639.
63. +T lymphocytes in HIV-1 infection as measured by Ki-67 antigen. J. Exp. Med. 187, 1295-1303.
64. Sasaki, K., Murakami, T., Kawasaki, M., and Takahashi, M. (1987) The cell cycle associated change of the Ki-67 reactive nuclear antigen expression. J. Cell. Physiol. 133, 579-584.
65. Schimenti, K. J., and Jacobberger, J. W. (1992). Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence. Cytometry 13, 48-59.
66. Schlüter, C., Duchrow, M., Wohlenberg, C., Becker, M. H., Key, G., Flad, H. D., and Gerdes, J. (1993). The cell proliferation-associated antigen of antibody Ki-67: A very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J. Cell Biol. 123, 513-522.
67. Scholzen, T., and Gerdes, J. (2000). The Ki-67 protein: From the known and the unknown. J. Cell. Physiol. 182, 311-322.
68. Schutte, B., Tinnemans, M. M., Pijpers, G. F., Lenders, M. H., and Ramaekers, F. C. (1995). Three parameter flow cytometric analysis for simultaneous detection of cytokeratin, proliferation associated antigens and DNA content. Cytometry 21, 177-186.
69. Schwarting, R. (1993). Little missed markers and Ki-67. Lab. Invest. 68, 597-599.
70. Schwarting, R., Gerdes, J., Niehus, J., Jaeschke, L., and Stein, H. (1986). Determination of the growth fraction in cell suspensions by flow cytometry using the monoclonal antibody Ki-67. J. Immunol. Methods 90, 65-70.
71. Scott, R. J., Hall, P. A., Haldane, J. S., van Noorden, S., Price, Y., Lane, D. P., and Wright, N. A. (1991). A comparison of immunohistochemical markers of cell proliferation with experimentally determined growth fraction. J. Pathol. 165, 173-178.
72. Shi, S. R., Imam, S. A., Young, L., Cote, R. J., and Taylor, C. R. (1995). Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies. J. Histochem. Cytochem. 43, 193-201.
73. Sprenger, R., Toellner, K. M., Schmelz, C, Lüke, W., Stahl-Henning. C., Ernst, M., Hunsmann, G., Schmitz, H., Flad, H. D., Gerdes, J., and Zimmer, J. P. (1995). Folicular dendritic cells productively infected with immunodeficiency viruses transmit infection to T cells. Med. Microbiol. Immunol. 184, 129-134.
74. Starborg, M., Gell, K., Brundell, E., and Höög, C. (1996). The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J. Cell Sci. 109, 143-153.
75. Takagi, M., Matsuoka, Y., Kurihara, T., and Yoneda, Y. (1999). Chmadrin: A novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure. J. Cell Sci. 112, 2463-2472.
76. Tsurusawa, M., and Fujimoto, T. (1995). Cell cycle progression and phenotypic modification of Ki67 antigen-negative G1- and G2-phase cells in phorbol ester-treated Molt-4 human leukemia cells. Cytometry 20, 146-153.
77. Van Dierendonck, J. H., Keijzer, R., van de Velde, C. J., and Cornelisse, C. J. (19S9). Nuclear distribution of the Ki-67 antigen during the cell cycle: Comparison with growth fraction in human breast cancer cells. Cancer Res. 49, 2999-3006.
78. Van Dierendonck, J. H., Wijsman, J. H., Keijzer, R., van de Velde, C. J., and Cornelisse, C. J. (1991). Cell-cycle-related staining patterns of anti-proliferating cell nuclear antigen monoclonal antibodies. Comparison with BrdUrd labeling and Ki-67 staining. Am. J. Pathol. 138, 1165-1172.
79. Verheijen, R., Kuijpers, H. J., van Driel, R., Beck, J. L., van Dierendonck, J. H., Brakenhoff, G. J., and Ramaekers, F. C. (1989a). Ki-67 detects a nuclear matrix-associated proliferation-related antigen. II. Localization in mitotic cells and association with chromosomes. J. Cell Sci. 92, 531-540.
80. Verheijen, R., Kuijpers, H. J., Schlingemann, R. O., Boehmer, A. L., van Driel, R., Brakenhoff, G. J., and Ramaekers, F. C. (1989b). Ki-67 detects a nuclear matrix-associated proliferation-related antigen. I. Intracellular localization during interphase. J. Cell Sci. 92, 123-130.
81. Vollmer, R. T., Humphrey, P. A., Swanson, P. E., Wick, M. R., and Hudson, M. L. (1998). Invasion of the bladder by transitional cell carcinoma: Its relation to histologic grade and expression of p53, MIB-1, c-erb B-2, epidermal growth factor receptor, and bcl-2. Cancer 82, 715-723.
82. Wersto, R. P., Herz, F., Gallagher, R. E., and Koss, L. G. (1988). Cell cycle-dependent reactivity with the monoclonal antibody Ki-67 during myeloid cell differentiation. Exp. Cell Res. 179, 79-88.
83. Wilson, G. D., Saunders, M. I., Dische, S., Daley, F. M., Robinson, B. M., Martindale, C. A., Joiner, B., and Richman, P. I. (1996). Direct comparison of bromodeoxyuridine and Ki-67 labelling indices in human tumours. Cell Prolif. 29, 141-152.