Spontaneous and MNNG-induced reversion of an EGFP construct in HeLa cells: An assay for observing mutations in living cells by fluorescent microscopy

Human Mutation

Volumn 18, Issue 6, 2001, Pages 526-534

  Gemignani, Federica ^a,c   Landi, Stefano ^b   De David, M ^a   Kole, Ryszard ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^c INTERNATIONAL AGENCY FOR RESEARCH ON CANCER   (France)

Author keywords

thalassemia; Aberrant splicing; EGFP; Enhanced green fluorescence protein; HBB; HeLa; MNNG; Mutation spectra; N methyl N nitro N nitrosoguanidine; Reversion assay

Indexed keywords

GREEN FLUORESCENT PROTEIN; METHYLNITRONITROSOGUANIDINE;

ARTICLE; CELL CULTURE; CELL MUTANT; CULTURE MEDIUM; DNA POLYMORPHISM; FLUORESCENCE MICROSCOPY; GENE DELETION; GENE EXPRESSION; GENE INSERTION; GENE MUTATION; HELA CELL; HUMAN; HUMAN CELL; PHENOTYPE; PRIORITY JOURNAL;

BASE SEQUENCE; CELL DIVISION; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; METHYLNITRONITROSOGUANIDINE; MICROSCOPY, FLUORESCENCE; MUTATION; RECOMBINANT FUSION PROTEINS;

MAMMALIA;

EID: 0035209888     PISSN: 10597794     EISSN: None     Source Type: Journal    
DOI: 10.1002/humu.1229     Document Type: Article

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