Requirement of nickel metabolism proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori

Molecular Microbiology

Volumn 39, Issue 1, 2001, Pages 176-182

  Olson, J W ^a   Mehta, N S ^a   Maier, R J ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; HYDROGENASE; KANAMYCIN; MUTANT PROTEIN; NICKEL; PROTEIN HYPA; PROTEIN HYPB; UNCLASSIFIED DRUG; UREASE;

ARTICLE; BACTERIAL GROWTH; CULTURE MEDIUM; ENZYME ACTIVITY; GENE CASSETTE; GENE INACTIVATION; GENOME; HELICOBACTER PYLORI; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; STRUCTURAL GENE;

BACTERIAL PROTEINS; CARRIER PROTEINS; GENES, BACTERIAL; GTP-BINDING PROTEINS; HELICOBACTER PYLORI; HYDROGENASE; METALLOPROTEINS; MUTAGENESIS, INSERTIONAL; MUTATION; NICKEL; UREASE;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI; PROKARYOTA;

EID: 0035191007     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02244.x     Document Type: Article

References (25)

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3. HypB protein of Bradyrhizobium japonicum is a metal-binding GTPase capable of binding 18 divalent nickel ions per dimer
4. Nickel enzymes in microbes
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6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
7. Purification and characterization of Klebsiella aerogenes UreE protein: A nickel-binding protein that functions in urease metallocenter assembly
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10. Isolation of Helicobacter pylori genes that modulate urease activity
11. Dissection of the maturation reactions of the [NiFe] hydrogehase 3 from Escherichia coli taking place after nickel incorporation
12. Hydrogen uptake hydrogenase in Helicobacter pylori
13. Nickel incorporation into hydrogenases
14. GTP hydrolysis by HypB is essential for nickel insertion into hydrogenases of Escherichia coli
15. Structure/function relationships in nickel metallobiochemistry
16. Characterization of urease from Campylobacter pylori
17. Helicobacter pylori nickel-transport gene nixA: Synthesis of catalytically active urease in Escherichia coli independent of growth conditions
18. Dual roles of Bradyrhizobium japonicum nickelin protein in nickel storage and GTP-dependent Ni mobilization
19. The HypB protein from Bradyrhizobium japonicum can store nickel and is required for the nickel-dependent transcriptional regulation of hydrogenase
20. Molecular analysis of a microaerobically induced operon required for hydrogenase synthesis in Rhizobium leguminosarum biovar viciae
21. Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis
22. GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins
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24. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
25. Phenol-hypochlorite reaction for determination of ammonia