Highly efficient single molecule detection in different micro and submicrometer channels with cw-excitation

Single Molecules

Volumn 2, Issue 3, 2001, Pages 165-175

  Dörre, Klaus ^a   Stephan, Jens ^a   Eigen, Manfred ^b  

^a EPFL   (Switzerland)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DYE;

ARTICLE; CALCULATION; CONTROLLED STUDY; DEVICE; DIFFUSION; EVALUATION; MEASUREMENT; METHODOLOGY; MOLECULAR PROBE; MOLECULE; PATCH CLAMP; PHOTON; PIPETTE; SIGNAL NOISE RATIO; TECHNIQUE;

EID: 0035178553     PISSN: 14385163     EISSN: None     Source Type: Journal    
DOI: 10.1002/1438-5171(200110)2:3<165::AID-SIMO165>3.0.CO;2-G     Document Type: Article

References (14)

1. Rigler R, Mets Ü. Diffusion of single molecules through a gaussian laser beam. SPIE 1921 (1992) 239-242
2. Dickson RM, Norris DJ, Tzeng YL, Moerner WE. Three-dimensional imaging of single molecules solvated on pores of polyacrylamide gels. Science 274 (1996) 966-969
3. Barnes MD, Whitten WB, Ramsey JM. Detecting single molecules in liquids. Anal Chem 67 (1995) A 418-A 423
4. Zander C, Sauer M, Drexhage KH, Ko DS, Schulz A, Wolfrum J, Brand L, Eggeling C, Seidel CAM. Detection and characterisation of single molecules in aqueous solution. Appl Phys B 63 (1996) 517-523
5. Eigen M, Rigler R. Sorting single molecules: application to diagnostics and evolutionary biotechnology. Proc Natl Acad Sci 91 (1994) 5740-5747
6. Keller RA, Ambrose WP, Goodwin PM, Jett JH, Martin JC, Wu M. Single molecule fluorescence analysis in solution. Appl Spectrosc 50 (1996) A 12-A 32
7. Basche T, Moerner WE. Optical modification of a single impurity molecule in a solid. Nature, 355 (1992) 335-337
8. Ha T, Enderle T, Ogletree DF, Chemla DS, Selvin PR, Weiss S. Probing the interaction between two single mlecules - fluorescence resonance energy transfer between a single donor and acceptor. Proc Natl Acad Sci 93 (1996) 6264-6268
9. Schäfer B, Gemeinhardt H, Uhl V, Greulich KO. Single Molecule DNA Restriction Analysis in the Light Microscope. Single Mol 1 (2000) 33-40
10. Schwille P, Meyer-Almes FJ, Rigler R. Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J 72 (1997) 1878-1886
11. Kettling U, Koltermann A, Schwille P, Eigen M. Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc Natl Acad Sci 95 (1998) 1416-1420
12. Koltermann A, Kettling U, Bieschke J, Winkler T, Eigen M. Rapid assay processing by integration of dual-color fluorescence cross-correlation spectroscopy: High throughput screening for enzyme activity. Proc Natl Acad Sci 95 (1998) 1421-1426
13. Winkler T, Kettling U, Koltermann A, Eigen M. Confocal fluorescence coincidence analysis: An approach to ultra high-throughput screening. Proc Natl Acad Sci 96 (1999) 1375-1378
14. Rigler R, Földes-Papp Z, Meyeralme FJ, Sammet C, Volcker M, Schnetz A. Fluorescence Cross-Correlation -a new concept for polymerase chain reaction. J Biotech 63 (1998) 97-109