Targeted gene disruption of the avermectin B O-methyltransferase gene in Streptomyces avermitilis

Biotechnology Letters

Volumn 23, Issue 21, 2001, Pages 1765-1770

  Hong, Young Soo ^a,b   Lee, Jeong Hyung ^a   Kim, Hang Sub ^a   Kim, Kyu Won ^b   Lee, Jung Joon ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Pusan National University   (South Korea)

Author keywords

Avermectin; Avermectin O methyltransferase; Gene disruption

Indexed keywords

ABAMECTIN; AVERMECTIN A; AVERMECTIN B O METHYLTRANSFERASE; AVERMECTIN DERIVATIVE; METHYLTRANSFERASE; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; ARTICLE; BACTERIAL GENETICS; DRUG STRUCTURE; GENE DISRUPTION; GENE FUNCTION; GENETIC ANALYSIS; NONHUMAN; PHENOTYPE; SOUTHERN BLOTTING; STREPTOMYCES; STREPTOMYCES AVERMITILIS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); STREPTOMYCES; STREPTOMYCES AVERMITILIS; UNCULTURED ACTINOMYCETE;

EID: 0035175842     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1012496317299     Document Type: Article

References (16)

1. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.
2. Avermectins, new family of potent anthelmintic agents: Producing organism and fermentation
3. An improved aminoglycoside resistance gene cassette for use in Gram-negative bacteria and Streptomyces
4. Biotransformation of milbemycin D to milbemycin G by Streptomyces lividans conferring avermectin O-methyltransferase activity
5. Genetic studies of avermectin biosynthesis in Streptomyces avermitilis
6. Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis
7. Direct production of 5-oxo derivatives of avermectins by a recombinant strain of Streptomyces avermitilis
8. Construction of a single component producer from the wild type avermectin producer Streptomyces avermitilis
9. Cloning of the gene encoding avermectin B 5-O-methyltransferase in avermectin-producing Streptomyces avermitilis
10. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector
11. Deletion analysis of the avermectin biosynthetic genes of Streptomyces avermitilis by gene cluster displacement
12. Selective production of specific components of avermectins in Streptomyces avermitilis
13. Avermectin B2 O-methyltransferase activity in Streptomyces avermitilis mutants that produce increased amounts of the avermectins
14. Streptomyces avermitilis mutants defective in methylation of avermectins