Palmitoylation of the Rous sarcoma virus transmembrane glycoprotein is required for protein stability and virus infectivity

Journal of Virology

Volumn 75, Issue 23, 2001, Pages 11544-11554

  Ochsenbauer Jambor, C ^a   Miller, D C ^a   Roberts, C R ^a   Rhee, S S ^a   Hunter, E ^a  

^a University of Alabama at Birmingham   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; GLYCINE; MEMBRANE PROTEIN; PALMITIC ACID; RNA DIRECTED DNA POLYMERASE; VIRUS GLYCOPROTEIN;

ACYLATION; ANIMAL CELL; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; CORRELATION FUNCTION; ENDOSOME; GENE EXPRESSION; GENE MUTATION; HYDROPHOBICITY; INTERNALIZATION; LYSOSOME; NONHUMAN; PALMITOYLATION; PHENOTYPE; PRECURSOR; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STABILITY; PROTEIN SYNTHESIS; ROUS SARCOMA ONCOVIRUS; STEADY STATE; VIRION; VIRUS GENOME; VIRUS INFECTION;

AMINO ACID SEQUENCE; ANIMALS; AVIAN SARCOMA VIRUSES; CELLS, CULTURED; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE PRODUCTS, ENV; MOLECULAR SEQUENCE DATA; PALMITIC ACID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION; TURKEYS; VIRAL ENVELOPE PROTEINS; VIRULENCE;

EID: 0035173062     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.23.11544-11554.2001     Document Type: Article

References (62)

1. Structural requirements of a membrane-spanning domain for protein anchoring and cell surface transport
2. Inhibition of the receptor-mediated endocytosis of diferric transferrin is associated with the covalent modification of the transferrin receptor with palmitic acid
3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels
4. Processing of gPr92env, the precursor to the glycoproteins of Rous sarcoma virus: Use of inhibitors of oligosaccharide trimming and glycoprotein transport
5. A single amino acid change in the cytoplasmic domain alters the polarized delivery of influenza virus hemagglutinin
6. Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
7. Measles virus fusion protein is palmitoyiated on transmembrane-intracytoplasmic cysteine residues which participate in cell fusion
8. Transferrin receptor internalization sequence YXRF implicates a tight turn as the structural recognition motif for endocytosis
9. A charged amino acid substitution within the transmembrane anchor of the Rous sarcoma virus envelope glycoprotein affects surface expression but not intracellular transport
10. Mutations in the leucine zipper of the human immunodeficiency virus type 1 transmembrane glycoprotein affect fusion and infectivity
11. Oligomeric structure of a prototype retrovirus glycoprotein
12. Evidence that a precursor glycoprotein is cleaved to yield the major glycoprotein of avian tumor virus
13. Site-directed mutations in the Sindbis virus 6K protein reveal sites for fatty acylation and the underacylated protein affects virus release and virion structure
14. Site-directed mutations in Sindbis virus E2 glycoprotein's cytoplasmic domain and the 6K protein lead to similar defects in virus assembly and budding
15. Fatty acylation of rabies virus proteins
16. Rous sarcoma virus p19 and gp35 can be chemically crosslinked to high molecular weight complexes. An insight into virus assembly
17. Preparation of RNA from eukaryotic and prokaryotic cells
18. Lipid domain structure of the plasma membrane revealed by patching of membrane components
19. Adenovirus death protein, a transmembrane protein encoded in the E3 region, is palmitoylated at the cytoplasmic tail
20. Synthesis and processing of avian sarcoma virus glycoproteins
21. Localization of the palmitoylation site in the transmembrane protein p12E of friend murine leukaemia virus
22. The DF-1 chicken fibroblast cell line: Transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses
23. Biological techniques for avian sarcoma viruses
24. Membrane insertion and transport of viral glycoproteins: A mutational analysis
25. Complete sequence of the Rous sarcoma virus env gene: Identification of structural and functional regions of its product
26. Retrovirus envelope glycoproteins
27. Site-directed mutations in the Sindbis virus E2 glycoprotein identify palmitoylation sites and affect virus budding
28. Nonacylated human transferrin receptors are rapidly internalized and mediate iron uptake
29. Transport of a lysosomally targeted Rous sarcoma virus envelope glycoprotein involves transient expression on the cell surface
30. New procedure for DNA transfection with polycation and dimethyl sulfoxide
31. Modified protocol for DNA sequence analysis using sequenase® 2.0
32. Role of fatty acid acylation of membrane glycoproteins. Absence of palmitic acid in glycoproteins of two serotypes of vesicular stomatitis virus
33. Fatty acid acylation at the single cysteine residue in the cytoplasmic domain of the glycoprotein of vesicular-stomatitis virus
34. Release of fatty acids from virus glycoproteins by hydroxylamine
35. Basolateral sorting of LDL receptor in MDCK cells: The cytoplasmic domain contains two tyrosine-dependent targeting determinants
36. Role of lipid modifications in targeting proteins to detergentresistant membrane rafts. Many raft proteins are acylated, while few are prenylated
37. Fatty acids on the A/Japan/305/57 influenza virus hemagglutinin have a role in membrane fusion
38. Effects of altering palmitoylation sites on biosynthesis and function of the influenza virus hemagglutinin
39. The Rous sarcoma virus Env glycoprotein contains a highly conserved motif homologous to tyrosine-based endocytosis signals and displays an unusual internalization phenotype
40. Intracellular aspects of the process of protein synthesis
41. Similarities among retrovirus proteins
42. Mutants of the Rous sarcoma virus envelope glycoprotein that lack the transmembrane anchor and cytoplasmic domains: Analysis of intracellular transport and assembly into virions
43. Mutations within the proteolytic cleavage site of the Rous sarcoma virus glycoprotein that block processing to gp85 and gp37
44. Assessment of fusogenic properties of influenza virus hemagglutinin deacylated by site-directed mutagenesis and hydroxylamine treatment
45. Domain-structure of cytoplasmic border region is main determinant for palmitoylation of influenza virus hemagglutinin (H7)
46. Fatty acylation of proteins: New insights into membrane targeting of myristoylated and palmitoylated proteins
47. The presence of cysteine in the cytoplasmic domain of the vesicular stomatitis virus glycoprotein is required for palmitate addition
48. The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors
49. Palmitoylation of cellular and viral proteins
50. Chemical identification of cysteine as palmitoylation site in a transmembrane protein (Semliki Forest virus E1)
51. Fatty acylation of proteins
52. Nucleotide sequence of Rous sarcoma virus
53. Functional rafts in cell membranes
54. Membrane association and defective transport of spleen focus-forming virus glycoproteins
55. Deacylation of the hemagglutinin of influenza A/Aichi/2/68 has no effect on membrane fusion properties
56. Structural requirements for high efficiency endocytosis of the human transferrin receptor
57. The M2 protein of influenza A virus is acylated
58. Different palmitoylation of paramyxovirus glycoproteins
59. Fatty acid acylation is not required for membrane fusion activity or glycoprotein assembly into VSV virions
60. Mutations of the Rous sarcoma virus env gene that affect the transport and subcellular location of the glycoprotein products
61. Palmitoylation of the murine leukemia virus envelope glycoprotein transmembrane subunits
62. The human and simian immunodeficiency virus envelope glycoprotein transmembrane subunits are palmitoylated