Aggrecan gene expression of chondrocyte-seeded 3D hydrogel cultures in response to hypertonic loading

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

Volumn 50, Issue , 2001, Pages 205-206

  Chao, Pen Hsiu G ^a   Palmer, Glyn D ^a   Mauck, Robert L ^a   Guo, X Edward ^a   Hung, Clark T ^a  

^a Columbia University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGELS; MONOLAYERS; PROTEINS; RNA;

AGGRECAN GENE EXPRESSION; HYPERTONIC LOADING;

GENES;

EID: 0242441657     PISSN: 10716947     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (11)

1. Urban JPG, Hall AC and Gehl KA. Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes. J Cell Physiol 1993; 154:262-70.
2. Borghetti P, Salda LD, De Angelis E, Maltarello MC, Petronini PG, Cabassi E, Marcato PS, Maraldi NM and Borghetti AF. Adaptive cellular response to osmotic stress in pig articular chondrocytes. Tissue Cell 1995; 27(2):173-183.
3. Palmer GD, Chao PG, Raia F, Valhmu WB, and Hung CT. Osmotic loading regulates chondrocyte cell size and aggrecan gene expression. Trans Orthop Res Soc 2000; 25:294.
4. Chao PG, Palmer GD, Valhmu WB, Thomas JL and Hung CT. Characterization of the chondrocyte response to hyperosmotic loading - influence of medium constituents. Trans. Orthop. Res. Soc 2001, accepted.
5. Benya PD and Shaffer JD. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 1982; 30:215-24.
6. Mauck RL, Soltz MA, Wang CC, Wong DD, Chao PH, Valhmu WB, and Hung CT. Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J Biomech Eng 2000; 122(3):252-60
7. Re P, Valhmu WB, Vostrejs M, Howell DS, Fisher SG and Ratcliffe A. Quantitative polymerase chain reaction assay for aggrecan and link protein gene expression in cartilage. Anal Biochem 1995; 225:356-60.
8. Paige KT, Cima LG, Yaremchuk MJ, Schloo BL, Vacanti JP, and Vacanti CA. De novo cartilage generation using calcium alginate-chondrocyte constructs. Plast Reconstr Surg 1996; 97(1):168-78; discussion 179-80
9. Wang CC-B, Soltz MA, Mauck RL, Valhmu WB, Ateshian GA and Hung CT. Comparison of equilibrium axial strain distributions in aricular cartilage explants and cell-seeded alginate disks under unconfined compression. Trans. Orthop. Res. Soc. 2000; 25:131.
10. Lai WM, Hou JS and Mow VC. A triphasic theory for the swelling and deformational behaviors of articular cartilage. J. Biomech. Eng. 1991; 113:245-258.
11. Mow VC, Sun DN, Guo XE, Hung CT and Lai WM. Chondrocyte-extracellular matrix interactions during osmotic swelling. Advances in Bioengineering 1999; BED-42:133-134.