Transfer protein TraY of plasmid R1 stimulates TraI-catalyzed oriT cleavage in vivo

Journal of Bacteriology

Volumn 183, Issue 3, 2001, Pages 909-914

  Karl, W ^a   Bamberger, M ^a   Zechner, E L ^a  

^a UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; INTEGRATION HOST FACTOR; PROTEIN TRAM; PROTEIN TRAY; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CONJUGATION; DNA TRANSFER; ESCHERICHIA COLI; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DNA BINDING;

BACTERIAL PROTEINS; CONJUGATION, GENETIC; DNA, BACTERIAL; DNA-BINDING PROTEINS; ENDODEOXYRIBONUCLEASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MODELS, GENETIC; PROTEIN BINDING; R FACTORS; REPLICATION ORIGIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035156081     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.3.909-914.2001     Document Type: Article

References (49)

1. Specific DNA binding of the TraM protein to the oriT region of plasmid R100
2. Requirements for mobilization of plasmids RSF1010 and ColE1 by the IncW plasmid R388: trwB and RP4 traG are interchangeable
3. Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation
4. Cointegrate formation mediated by Tn9. II. Activity of IS1 is modulated by external DNA sequences
5. Biochemical characterization of Escherichia coli DNA helicase I
6. Key regulatory aspects of transfer of F-related plasmids
7. Regulation of R100 conjugation requires traM in cis to traJ
8. The TraM protein of the conjugative plasmid F binds to the origin of transfer of the F and ColE1 plasmids
9. The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro
10. Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F
11. Mobilization of chimeric oriT plasmids by F and R100-1: Role of relaxosome formation in defining plasmid specificity
12. Conjugative transfer of promiscuous plasmid RP4: Plasmid-specified functions essential for formation of relaxosomes
13. A point mutation in the Nul gene of bacteriophage lambda facilitates phage growth in Escherichia coli with himA and gyrB mutations
14. The sequence of the leading region of the resistance plasmid R1
15. TraG from RP4 and TraG and VirD4 from Ti plasmids confer relaxosome specificity to the coniugal transfer system of pTiC58
16. Stepwise assembly of a relaxosome at the F plasmid origin of transfer
17. Site- and strand-specific nicking at oriT of plasmid R100 in a purified system: Enhancement of the nicking activity of TraI (helicase I) with TraY and IHF
18. Specific binding of the TraY protein to oriT and the promoter region for the tray gene of plasmid R100
19. The requirements for conjugal DNA synthesis in the donor strain during Flac transfer
20. Transfer protein TraM stimulates TraI-catalyzed cleavage of the transfer origin of plasmid R1 in vivo
21. Purified Escherichia coli F-factor TraY protein binds oriT
22. Dissection of IncP conjugative plasmid transfer: Definition of the transfer region Tra2 by mobilization of the Tra1 region in trans
23. Mutational and physical analysis of F plasmid traY protein binding to oriT
24. Construction of derivatives of the F plasmid pOX-tra715: Characterization of traY and traD mutants that can be complemented in trans
25. Transfer-deficient cointegrates of Flac and lambda prophage
26. Characterization of ATP and DNA binding activities of TrwB, the coupling protein essential in plasmid R388 conjugation
27. The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalyzed nicking at the F plasmid oriT
28. The F plasmid traY gene product binds DNA as a monomer or a dimer: Structural and functional implications
29. Characterization of the Escherichia coli F factor traY gene product and its binding sites
30. In vitro assembly of relaxosomes at the transfer origin of plasmid RP4
31. Concerted action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (TraI) of conjugative plasmid RP4
32. Relaxase (TraI) of IncPα plasmid RP4 catalyzes a site-specific cleaving-joining reaction of single-stranded DNA
33. Regulation of the expression of the traM gene of the F sex factor of Escherichia coli
34. TraM of plasmid R1 controls transfer gene expression as an integrated control element in a complex regulatory network
35. The carboxyl terminus of protein TraD adds specificity and efficiency to F-plasmid conjugative transfer
36. In vitro cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins
37. The TraM protein of plasmid R1 is a DNA-binding protein
38. Tram of plasmid R1 regulates its own expression
39. Escherichia coil DNA helicase I catalyzes a sequence-specific cleavage/ligation reaction at the F plasmid origin of transfer
40. Effect of traY amber mutations on F-plasmid traY promoter activity in vivo
41. Sequence identity in the nick regions of IncP plasmid transfer origins and T-DNA borders of Agrobacterium Ti plasmids
42. Sites and systems for conjugal DNA transfer in bacteria
43. Conjugative-DNA transfer processes
44. Specific cleavage of chromosomal and plasmid DNA strands in gram-positive and gram-negative bacteria can be detected with nucleotide resolution
45. Localized denaturation of oriT DNA within relaxosomes of the broad-host-range plasmid R1162
46. The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer
47. TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence repetition within the transfer origin
48. TraK protein of conjugative plasmid RP4 forms a specialized nucleoprotein complex with the transfer origin