Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy

Human Reproduction

Volumn 16, Issue 11, 2001, Pages 2374-2378

  Wang, Wei Hua ^a,b,d   Meng, Li ^a,b   Hackett, Rickard J ^a,b   Odenbourg, Rudolf ^c   Keefe, David L ^a,b,c  

^a BROWN UNIVERSITY   (United States)

^b TUFTS MEDICAL CENTER   (United States)

^c MARINE BIOLOGICAL LABORATORY   (United States)

^d WOMEN AND INFANTS HOSPITAL   (United States)

Author keywords

Cooling; Human oocytes; Polarization microscope; Spindle

Indexed keywords

ARTICLE; BIREFRINGENCE; COOLING; EMBRYO DEVELOPMENT; FERTILIZATION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IN VITRO STUDY; KINETICS; MEIOSIS; METHODOLOGY; MICROSCOPY; MICROTUBULE; MITOSIS SPINDLE; OOCYTE; TEMPERATURE SENSITIVITY; WARMING;

EID: 0035155205     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/16.11.2374     Document Type: Article

References (18)

1. Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-matured bovine oocytes
2. The effect of temperature fluctuations on the cytoskeletal organization and chromosomal constitution of the human oocyte
3. Influence of maternal age on meiotic spindle in oocytes from naturally cycling women
4. Aneuploidy 16 in human embryos increases significantly with maternal age
5. The microtubular cytoskeleton and chromosomes of unfertilized human oocytes aged in vitro
6. A reliable, noninvasive technique for spindle imaging and enucleation of mammalian oocytes
7. Increased birefringence in the meiotic spindle provides a new marker for the onset of activation in living oocytes
8. Temperature-induced abnormalities in sheep oocytes during maturation
9. Preimplantation genetic diagnosis of numerical abnormalities using FISH
10. A new view on polarization microscopy
11. Polarized light microscopy of spindles
12. The influence of cooling on the organization of the meiotic spindle of the mouse oocyte
13. Transient cooling to room temperature can cause irreversible disruption to the meiotic spindle in human oocytes
14. The first polar body does not predict accurately the location of the metaphase II meiotic spindle in mammalian oocytes
15. The meiotic competence of in-vitro matured human oocytes is influenced by donor age: Evidence that folliculogenesis is compromised in the reproductively aged ovary
16. Spindle observation and its relationship with fertilization after ICSI in living human oocytes
17. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination
18. Effects of chilling to 0°C on the morphology of meiotic spindles in human metaphase II oocytes