Mucin biosynthesis and secretion in tracheal epithelial cells in primary culture

Biochemical Journal

Volumn 353, Issue 1, 2001, Pages 23-32

  Svitacheva, N ^a   Davies, J R ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Airway; Bovine trachea; Cell culture; Mucus; Radio labelling

Indexed keywords

CHONDROITIN ABC LYASE; COLLAGEN TYPE 1; GLYCOSYLATED PROTEIN; HEPARAN SULFATE; HEPARAN SULFATE LYASE; MUCIN; PLASTIC; PROLINE; PROTEOGLYCAN; SIALIC ACID; SULFATE; TRYPSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL GROWTH; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DISULFIDE BOND; ISOTOPE LABELING; NONHUMAN; OLIGOMERIZATION; PERIODIC ACID SCHIFF STAIN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; TISSUE CULTURE CELL; TRACHEA MUCOSA;

ANIMALIA; BOVINAE;

EID: 0035152425     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/bj3530023     Document Type: Article

References (36)

1. Studies on the 'insoluble' glycoprotein complex from human colon. Identification of reduction-insensitive MUC2 oligomers and C-terminal cleavage
2. Different mucins are produced by the surface epithelium and the submucosa in human trachea: Identification of MUC5AC as a major mucin from the goblet cells
3. MUC5B is a major gel-forming, oligomeric mucin from human salivary gland, respiratory tract and endocervix: Identification of glycoforms and C-terminal cleavage
4. Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin
5. Genomic organization and structure of the 3′ region of human MUC3: Alternative splicing predicts membrane-bound and soluble forms of the mucin
6. Complete sequence of the human mucin MUC4: A putative cell membrane-associated mucin
7. Two novel mucin genes down-regulated in colorectal cancer identified by differential display
8. Epithelial mucin genes
9. The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region
10. Molecular cloning of human gastric mucin MUC5AC reveals conserved cysteine-rich D-domains and a putative leucine zipper motif
11. Human mucin gene MUC5B, the 10.7 -kb large central exon encodes various alternate subdomains resulting in a super-repeat. Structural evidence for a 11p15.5 gene family
12. The carboxyl-terminal sequence of the human secretory mucin, MUC6. Analysis of the primary amino acid sequence
13. Expression of human mucin genes in respiratory, digestive, and reproductive tracts ascertained by in situ hybridization
14. MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions
15. MUC5B and MUC7 are differentially expressed in mucous and serous cells of submucosal glands in human bronchial airways
16. Airway mucin genes and gene products
17. Antibodies to the cytoplasmic domain of the MUC1 mucin show conservation throughout mammals
18. Alternative splicing generates a family of putative secreted and membrane-associated MUC4 mucins
19. Biosynthesis of mucins in bovine trachea: Identification of the major radiolabelled species
20. Mucins in cat airway secretions
21. Turnover of cell-surface macromolecules in cultured dog tracheal epithelial cells
22. Identification and characterization of high molecular-mass mucin-like glycoproteins in the plasma membrane of airway epithelial cells
23. Synthesis of sulfated oligosaccharides by cystic fibrosis trachea epithelial cells
24. IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo
25. Quantitation of mucus glycoproteins blotted onto nitrocellulose membranes
26. A sensitive microplate assay for glycoproteins that utilizes an immunological digoxigenin-based detection system
27. Mucins in airway secretions from healthy and chronic bronchitic subjects
28. Size heterogeneity of human cervical mucus glycoproteins. Studies performed with rate-zonal centrifugation and laser light-scattering
29. Phase separation of integral membrane proteins in Triton X-114 solution
30. Isolation and characterization of human cervical-mucus glycoproteins
31. Mucin synthesis and secretion by cultured tracheal cells: Effects of collagen gel substratum thickness
32. Mucus glycoproteins in bovine trachea: Identification of the major mucin populations in respiratory secretions and investigation of their tissue origins
33. Chromosomal localization of a human mucin gene (MUC8) and cloning of the cDNA corresponding to the carboxy terminus
34. Characterization of mucins from cultured normal human tracheobronchial epithelial cells
35. Secretory proteins and glycoconjugates synthesized by human tracheal gland cells in culture
36. Characterization of a unique mucin-like glycoprotein secreted by a human endometrial adenocarcinoma cell line