Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system

Journal of Molecular Microbiology and Biotechnology

Volumn 3, Issue 1, 2001, Pages 73-82

  Karimova, G ^a   Ullmann, A ^a   Ladant, D ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

LEUCINE ZIPPER PROTEIN; TYROSINE TRANSFER RNA LIGASE;

ARTICLE; GEOBACILLUS STEAROTHERMOPHILUS; MOLECULAR MODEL; NONHUMAN; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; TWO HYBRID SYSTEM;

ADENYLATE CYCLASE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BACILLUS STEAROTHERMOPHILUS; BASE SEQUENCE; BORDETELLA PERTUSSIS; DIMERIZATION; LEUCINE ZIPPERS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN STRUCTURE, TERTIARY; TWO-HYBRID SYSTEM TECHNIQUES; TYROSINE; TYROSINE-TRNA LIGASE;

BACTERIA (MICROORGANISMS); BORDETELLA PERTUSSIS; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0035151432     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Site-specific mutagenesis by using an accurate recombinant polymerase chain reaction method
2. Analyzing protein-protein interactions using two-hybrid system
3. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives
4. A model of synthetase/transfer RNA interaction as deduced by protein engineering
5. Discrimination between transfer-RNAs by tyrosyl-tRNA synthetase
6. Engineering the quaternary structure of an exported protein with a leucine zipper
7. Structure of tyrosyl-tRNA synthetase refined at 2.3 A resolution. Interaction of the enzyme with the tyrosyl adenylate intermediate
8. Crystal structure of a deletion mutant of a tyrosyl-tRNA synthetase complexed with tyrosine
9. A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli
10. Conversion of the omega subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target
11. A novel genetic system to detect protein-protein interactions
12. The two-hybrid system: An assay for protein-protein interactions
13. The anticodon-binding domain of tyrosyl-tRNA synthetase: State of folding and origin of the crystallographic disorder
14. Escherichia coli one-and two-hybrid systems for the analysis and identification of protein-protein interactions
15. Sequence requirements for coiled-coils: Analysis with lambda repressor-GCN4 leucine zipper fusions
16. Changes in the periplasmic linker and in the expression level affect the activity of ToxR and lambda-ToxR fusion proteins in Escherichia coli
17. A bacterial two-hybrid system based on a reconstituted signal transduction
18. Gene activation by the AraC protein can be inhibited by DNA looping between AraC and a LexA repressor that interacts with AraC: Possible applications as a two-hybrid system
19. Evidence that the leucine zipper is a coiled coil
20. The genetic control and cytoplasmic expression of inducibility in the synthesis of b-galactosidase of Escherichia coli
21. Experimental evolution of a dense cluster of residues in tyrosyl-tRNA synthetase: Quantitative effects on activity, stability and dimerization
22. Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments
23. Protein-protein interactions: Methods for detection and analysis
24. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae
25. Yeast forward and reverse 'n'-hybrid systems
26. Role of residue Glu152 in the discrimination between transfer RNAs by tyrosyl-tRNA synthetase from Bacillus stearothermophilus
27. Protein engineering of homodimeric tyrosyl-tRNA synthetase to produce active heterodimers
28. Effects of engineering complementary charged residues into the hydrophobic subunit interface of tyrosyl-tRNA synthetase. Appendix: Kinetic analysis of dimeric enzymes that reversibly dissociate into inactive subunits