Gene activation by the AraC protein can be inhibited by DNA looping between AraC and a LexA repressor that interacts with AraC: Possible applications as a two-hybrid system

Molecular Microbiology

Volumn 30, Issue 3, 1998, Pages 615-624

  Kornacker, M G ^a   Remsburg, B ^a   Menzel, R ^b  

^a BRISTOL MYERS SQUIBB   (United States)

^b Princeton Corporate Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CYTARABINE; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; DIMERIZATION; DNA CONFORMATION; ESCHERICHIA COLI; GENE ACTIVATION; GENE CONTROL; GENE REPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PROTEIN INTERACTION; REPORTER GENE;

ARAC TRANSCRIPTION FACTOR; BACTERIAL PROTEINS; DIMERIZATION; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, FOS; GENES, JUN; GENES, REPORTER; LAC OPERON; NUCLEIC ACID CONFORMATION; PLASMIDS; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SERINE ENDOPEPTIDASES; TRANSCRIPTION FACTORS;

ARA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031766018     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.01096.x     Document Type: Article

References (50)

1. Allen, J.B., Walberg, M.W., Edwards, M.C., and Elledge, S.J. (1995) Finding prospective partners in the library: the two-hybrid system and phage display find a match. Trends Biochem Sci 20: 511-516.
2. Amouyal, M., Mortensen, L., Buc, H., and Hammer, K. (1989) Single and double loop formation when DeoR repressor binds its natural operator sites. Cell 58: 545-551.
3. Buchheit, P.-O., Schmidt-Doerr, T., Granger-Schnarr, M., and Schnarr, M. (1993) Spacing requirements between LexA operator half-sites can be relaxed by fusing the LexA DNA binding domain with some alternative dimerization domains. J Mol Biol 229: 1-7.
4. Bustos, S.A., and Schleif, R.F. (1993) Functional domains of the AraC protein. Proc Natl Acad Sci USA 90: 5638-5642.
5. Chatterjee, S., Zhou, Y.-N., Roy, S., and Adhya, S. (1997) Interaction of Gal repressor with inducer and operator: induction of gal transcription from repressor-bound DNA. Proc Natl Acad Sci USA 94: 2957-2962.
6. Choy, H.E., and Adhya, S. (1992) Repression and activation of transcription by Gal and Lac repressors: involvement of alpha subunit of RNA polymerase. Proc Natl Acad Sci USA 89: 11264-11268.
7. Chugani, S.A., Parsek, M.R., and Chakrabarty, A.M. (1998) Transcriptional repression mediated by LysR-type regulator CatR bound at multiple binding sites. J Bacteriol 180: 2367-2372.
8. Churchward, G., Belin, D., and Nagamine, Y. (1984) A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene 31: 165-171.
9. Cowan, J.M., Urbanowski, M.L., Talmi, M., and Stauffer, G. (1993) Regulation of the Salmonella typhimurium metF gene by the MetR protein. J Bacteriol 175: 5862-5866.
10. Doerr, T.S., Oertel-Buchheit, P., Pernelle, C., Bracco, L., Schnarr, M., and Granger-Schnarr, M. (1991) Construction, purification, and characterization of a hybrid protein comprising the DNA binding domain of the LexA repressor and the Jun leucine zipper: a circular dichroism and mutagenesis study. Biochemistry 30: 9657-9664.
11. Dove, S.L., and Hochschild, A. (1998) Conversion of the ω subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target. Genes Dev 12: 745-754.
12. Dove, S.L., Joung, J.K., and Hochschild, A. (1997) Activation of prokaryotic transcription through arbitrary protein-protein contacts. Nature 386: 627-630.
13. Elledge, S.J., and Davis, R.W. (1989) Position and density effects on repression by stationary and mobile DNA binding proteins. Genes Dev 3: 185-197.
14. Fields, S., and Song, O.-K. (1989) A novel genetic system to detect protein-protein interactions. Nature 340: 245-246.
15. Flamm, E.L., and Weisberg, R.A. (1985) Primary structure of the hip gene of Escherichia coli and of its product, the beta subunit of integration host factor. J Mol Biol 183: 117-128.
16. Gibbs, J.B., and Oliff, A. (1994) Pharmaceutical research in molecular oncology. Cell 79: 193-198.
17. Giffard, P.M., and Booth, I.R. (1988) The rpoA341 allele of Escherichia coli specifically impairs the transcription of a group of positively-regulated operons. Mol Gen Genet 214: 148-152.
18. Golemis, E.A., and Brent, R. (1992) Fused protein domains inhibit DNA binding by LexA. Mol Cell Biol 12: 3006-3014.
19. Higgins, C.F. (1993) The multidrug resistance P-glycoprotein. Curr Opin Cell Biol 5: 684-687.
20. Hoover, T.R., Santero, S.P., and Kustu, S. (1990) The integration host factor stimulates interaction of RNA polymerase with NifA, the transcriptional activator for nitrogen fixation operons. Cell 63: 11-22.
21. Hu, J.C., O'Shea, E.K., Kim, P.S., and Sauer, R.T. (1990) Sequence requirements for coiled-coils: analysis with lambda repressor-GCN4 leucine zipper fusions. Science 250: 1400-1403.
22. Huibregtse, J.M., Scheffner, M., and Howley, P. (1991) A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 an 18. EMBO J 10: 4129-4135.
23. Huo, L.I., Martin, K.J., and Schleif, R. (1988) Alternative DNA loops regulate the arabinose operon in Escherichia coli. Proc Natl Acad Sci USA 85: 5444-5448.
24. Jappelli, R., and Brenner, S. (1996) Interaction between cAMP-dependent protein kinase catalytic subunit and peptide inhibitors analyzed with λ repressor fusions. J Mol Biol 259: 575-578.
25. Karimova, G., Pidoux, J., Ullman, A., and Ladant, D. (1998) A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proc Natl Acad Sci USA 95: 5752-5756.
26. Kim, B., and Little, J.W. (1992) Dimerization of a specific DNA binding protein on the DNA. Science 255: 203-206.
27. Kosturko, L.D., Daub, E., and Murialdo, H. (1989) The interaction of E. coli integration host factor and lambda cos DNA: multiple complex formation and protein-induced bending. Nucleic Acids Res 17: 317-334.
28. Kouzarides, T., and Ziff, E. (1989) Leucine zippers of fos, jun and GCN4 dictate dimerization specificity and thereby control DNA binding. Nature 340: 568-571.
29. Lee, D.-H., and Schleif, R.F. (1989) In vivo DNA loops in araBAD: size limits and helical repeat. Proc Natl Acad Sci USA 86: 476-480.
30. Lewis, L.K., Harlow, G.R., Gregg-Jolly, L.A., and Mount, D.W. (1994) Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli. J Mol Biol 241: 507-523.
31. Mandal, N., Su, W., Haber, R., Adhya, S., and Echols, H. (1990) DNA looping in cellular repression of transcription of the galactose operon. Genes Dev 4: 410-418.
32. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
33. Marchetti, A., Abril-Marti, M., Illi, B., Cesareni, G., and Nasi, S. (1995) Analysis of Myc and Max interaction specificity with λ repressor-HLH domain fusions. J Mol Biol 248: 541-550.
34. Matthews, K.S. (1992) DNA looping. Microbiol Rev 56: 123-136.
35. Meissner, P.S., Sisk, W.P., and Berman, M.L. (1987) Bacteriophage lambda cloning system for the construction of directional cDNA libraries. Proc Natl Acad Sci USA 84: 4171-4175.
36. Menzel, R. (1989) A microtiter plate-based system for the semiautomated growth and assay of bacterial cells for β-galactosidase activity. Anal Biochem 181: 40-50.
37. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
38. Mueller, J., Oehler, S., and Mueller-Hill, B. (1996) Repression of lac promoter as a function of distance, phase and quality of an auxiliary lac operator. J Mol Biol 257: 21-29.
39. Nakamura, H., and Suganuma, A. (1972) Membrane mutation associated with sensitivity to acriflavine in Escherichia coli. J Bacteriol 110: 329-335.
40. Nash, H.A. (1990) Bending and supercoiling of DNA at the attachment site of bacteriophage lambda. Trends Biochem Sci 15: 222-227.
41. Nickerson, C.A., and Achberger, E.C. (1995) Role of curved DNA in binding of Escherichia coli RNA polymerase to promoters. J Bacteriol 177: 5756-5761.
42. Ottemann, K.M., and Mekalanos, J.J. (1995) Analysis of Vibrio cholerae ToxR fusions by construction of novel fusion proteins. Mol Microbiol 15: 719-731.
43. Ramos, J.L., Rojo, F., Zhou, L., and Timmis, K.N. (1990) A family of positive regulators related to the Pseudomonas putida TOL plasmid XyIS and the Escherichia coli AraC activators. Nucleic Acids Res 18: 2149-2152.
44. Reeder, T., and Schleif, R. (1993) AraC protein can activate transcription from only one position and when pointed in only one direction. J Mol Biol 231: 205-218.
45. Rippe, K., von Hippel, P.H., and Langowski, J. (1995) Action at a distance: DNA-looping and initiation of transcription. Trends Biochem Sd 20: 500-506.
46. Ryseck, R.P., Kovary, K., and Bravo, R. (1990) Integrity of FOS B leucine zipper is essential for its interaction with JUN proteins. Oncogene 5: 1091-1093.
47. Schleif, R. (1996) In Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology, Neidhardt, F.C. (ed.). Washington, DC: American Society for Microbiology Press, pp. 1300-1309.
48. Skerra, A. (1994) Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151: 131-135.
49. Wang, J.C., and Giaever, G.N. (1988) Action at a distance along a DNA. Science 240: 300-306.
50. Yang, S.-W., and Nash, H. (1994) Specific photocrosslinking of DNA-protein complexes: identification of contacts between integration host factor and its target DNA. Proc Natl Acad Sci USA 91: 12183-12187.