Recombinant single chain cardiac troponin I-C polypeptides: Superior calibration and control materials for cardiac troponin I immunoassays

Clinical Laboratory

Volumn 47, Issue 1-2, 2001, Pages 19-27

  Liu, S ^a   Zhang, M Y ^a   Song, Q ^a   Ling, M ^a   Kadijevic, L ^a   Shi, Q ^a  

^a Spectral Diagnostics Inc   (Canada)

Author keywords

Calibrator; Control; Recombinant protein; Troponin I

Indexed keywords

MONOCLONAL ANTIBODY; POLYPEPTIDE; RECOMBINANT PROTEIN; TROPONIN C; TROPONIN I;

AFFINITY CHROMATOGRAPHY; ARTICLE; CALIBRATION; CLINICAL LABORATORY; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DEVICE; DISSOCIATION; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; HEART; HUMAN; IMMUNOASSAY; MATERIALS; MOLECULAR STABILITY; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; REPRODUCIBILITY; SERUM; TEMPERATURE;

ANTIBODIES, MONOCLONAL; CALIBRATION; CHROMATOGRAPHY, AFFINITY; DRUG STABILITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOASSAY; MYOCARDIUM; PEPTIDE FRAGMENTS; QUALITY CONTROL; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; TROPONIN C; TROPONIN I;

ESCHERICHIA COLI;

EID: 0035150489     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

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2. Degradation of cardiac troponin I in serum complicates comparisons of cardiac troponin I assays
3. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex
4. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I
5. Degradation of cardiac troponin I: Implication for reliable immunodetection
6. Analytical performance and clinical utility of a sensitive immunoassay for determination of human cardiac troponin I
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8. Cloning, structure analysis, and expression of the human slow twitch skeletal muscle/cardiac troponin C gene
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10. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
11. Cleavage of structural proteins during the assembly of the head of bacteriophage
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13. "Western blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gets to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
14. Determination of cardiac troponin I on Stratus analyzer: Prospective evaluation in unstable angina
15. Rapid and specific immunoassay for cardiac troponin I in the diagnosis of myocardial damage
16. Cardiac troponin I measurement with the Access immunoassay system: Analytical and clinical performance characteristics
17. Emergency room triage of patients with acute chest pain by means of rapid testing for cardiac troponin T or troponin I
18. Biochemical factors influencing measurement of cardiac troponin I in serum
19. Extra leader sequence affects immunoactivity of cardiac troponin I
20. Troponin complex for the preparation of troponin I calibrators and standards
21. Human cardiac troponin I: Precise identification of antigenic epitopes and prediction of secondary structure