Characterization of recombinant hepatitis A virus genomes containing exogenous sequences at the 2A/2B junction

Journal of Virology

Volumn 75, Issue 3, 2001, Pages 1414-1426

  Beard, M R ^a   Cohen, L ^a   Lemon, S M ^a   Martin, A ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYPEPTIDE; POLYPROTEIN; PROTEIN; RECOMBINANT DNA; VIRUS RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FETUS; HEPATITIS A VIRUS; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHESUS MONKEY; SEQUENCE HOMOLOGY; VIRUS GENOME;

ANIMALS; BASE SEQUENCE; CELLS, CULTURED; DNA TRANSPOSABLE ELEMENTS; GENOME, VIRAL; HEPATOVIRUS; MACACA MULATTA; MOLECULAR SEQUENCE DATA; RECOMBINATION, GENETIC; REPETITIVE SEQUENCES, NUCLEIC ACID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; VIRAL PROTEINS;

EID: 0035148494     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.3.1414-1426.2001     Document Type: Article

References (50)

1. Polioviruses containing picornavirus type 1 and/or type 2 internal ribosomal entry site elements: Genetic hybrids and the expression of a foreign gene
2. Morphogenesis of hepatitis A virus: Isolation and characterization of subviral particles
3. Poliovirus replicons that express the gag or the envelope surface protein of simian immunodeficiency virus SIV(smm) PBj14
4. Engineering poliovirus as a vaccine vector for the expression of diverse antigens
5. Initiation of translation of human rhinovirus RNA: Mapping the internal ribosome entry site
6. Picornavirus internal ribosome entry segments: Comparison of translation efficiency and the requirements for optimal internal initiation of translation in vitro
7. Sequences within the poliovirus internal ribosome entry segment control viral RNA synthesis
8. Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins
9. Synthesis and assembly of hepatitis A virus specific proteins in BS-C-1 cells
10. In vitro characterization of an internal ribosomal entry site (IRES) present within the 5′ nontranslated region of hepatitis A virus RNA: Comparison with the IRES of encephalomyocarditis virus
11. Intragenomic complementation of a 3AB mutant in dicistronic polioviruses
12. Complete nucleotide sequence of an attenuated hepatitis A virus: Comparison with wild-type virus
13. Hepatitis A virus cDNA and its RNA transcripts are infectious in cell culture
14. Effects of P2 cleavage site mutations on poliovirus polyprotein processing
15. Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation
16. Hepatitis A virus translation is rate-limiting for virus replication in MRC-5 cells
17. Identification of hepatitis A virus non-structural protein 2B and its release by the major virus protease 3C
18. Hepatitis A virus capsid protein VP1 has a heterogeneous C terminus
19. Primary cleavage of the HAV capsid protein precursor in the middle of the proposed 2A coding region
20. Preferred sites of recombination in poliovirus RNA: An analysis of 40 intertypic cross-over sequences
21. RNA recombination in animal and plant viruses
22. Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR
23. Type A viral hepatitis. New developments in an old disease
24. Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures
25. Internal ribosome entry sites within the RNA genomes of hepatitis C virus and other flaviviruses
26. Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: Evidence for genetic recombination
27. Construction and genetic analysis of dicistronic polioviruses containing open reading frames for epitopes of human immunodeficiency virus type 1 gp 120
28. Monoclonal antibodies against hepatitis A virus
29. pro proteinase
30. Identification and site-directed mutagenesis of the primary (2A/2B) cleavage site of the hepatitis A virus polyprotein: Functional impact on the infectivity of HAV RNA transcripts
31. The rhinovirus type 14 genome contains an internally located RNA structure that is required for viral replication
32. Cardioviral internal ribosomal entry site is functional in a genetically engineered dicistronic poliovirus
33. Studies on dicistronic polioviruses implicate viral proteinase 2Apro in RNA replication
34. Primary structure and gene organization of human hepatitis A virus
35. Detection and characterization of subgenomic RNAs in hepatitis A virus particles
36. Proteolytic processing of the cardioviral P2 region: Primary 2A/2B cleavage in clone-derived precursors
37. Encephalomyocarditis virus 3C protease: Efficient cell-free expression from clones which link viral 5′ noncoding sequences to the P3 region
38. Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999
39. Virus-encoded proteinases of the picornavirus super group
40. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence
41. Proteinase 3C of hepatitis A virus (HAV) cleaves the HAV polyprotein P2-P3 at all sites including VP1/2A and 2A/2B
42. Cleavage specificity of purified recombinant hepatitis A virus 3C proteinase on natural substrates
43. Mutations within the 5′ nontranslated RNA of cell culture-adapted hepatitis A virus which enhance cap-independent translation in cultured African green monkey kidney cells
44. Enzymatic inverse PCR: A restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis
45. Toward a poliovirus-based simian immunodeficiency virus vaccine: Correlation between genetic stability and immunogenicity
46. Detection of a genome-linked protein (VPg) of hepatitis A virus and its comparison with other picornaviral VPgs
47. Low efficiency of the 5′ nontranslated region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells
48. Genetics of poliovirus
49. An infectious cDNA clone of a cytopathic hepatitis A virus: Genomic regions associated with rapid replication and cytopathic effect
50. Characterization of replication-competent hepatitis A virus constructs containing insertions at the N terminus of the polyprotein